Construct: ORF TRCN0000479977
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001501.1_s317c1
- Derived from:
- ccsbBroadEn_06457
- DNA Barcode:
- TGGTCTTCCTACACCCCTGGCGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ITGA6 (3655)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479977
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3655 | ITGA6 | integrin subunit alpha 6 | NM_000210.4 | 99.9% | 99.9% | 1138G>A;2082C>T |
| 2 | human | 3655 | ITGA6 | integrin subunit alpha 6 | NM_001365530.2 | 98.5% | 98.5% | 1138G>A;2082C>T;2633_2634ins45 |
| 3 | human | 3655 | ITGA6 | integrin subunit alpha 6 | NM_001079818.3 | 96.2% | 95.8% | (many diffs) |
| 4 | human | 3655 | ITGA6 | integrin subunit alpha 6 | NM_001365529.2 | 94.8% | 94.5% | (many diffs) |
| 5 | human | 3655 | ITGA6 | integrin subunit alpha 6 | XM_017004007.1 | 89.3% | 89.2% | 0_1ins342;796G>A;1740C>T |
| 6 | human | 3655 | ITGA6 | integrin subunit alpha 6 | XM_017004008.1 | 87.9% | 87.8% | (many diffs) |
| 7 | human | 3655 | ITGA6 | integrin subunit alpha 6 | NM_001316306.2 | 86.3% | 86.1% | (many diffs) |
| 8 | human | 3655 | ITGA6 | integrin subunit alpha 6 | XM_017004005.1 | 85.8% | 85.4% | (many diffs) |
| 9 | human | 3655 | ITGA6 | integrin subunit alpha 6 | XM_017004006.1 | 84.4% | 84.1% | (many diffs) |
| 10 | mouse | 16403 | Itga6 | integrin alpha 6 | NM_008397.4 | 86.8% | 93.1% | (many diffs) |
| 11 | mouse | 16403 | Itga6 | integrin alpha 6 | XM_017315707.1 | 84.4% | 90.1% | (many diffs) |
| 12 | mouse | 16403 | Itga6 | integrin alpha 6 | NM_001277970.1 | 84.4% | 89.4% | (many diffs) |
| 13 | mouse | 16403 | Itga6 | integrin alpha 6 | XM_011239308.1 | 84.4% | 89.4% | (many diffs) |
| 14 | mouse | 16403 | Itga6 | integrin alpha 6 | XM_011239309.2 | 82% | 86.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3288
- ORF length:
- 3219
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccgccgcc gggcagctgt gcttgctcta cctgtcggcg gggctcctgt 121 cccggctcgg cgcagccttc aacttggaca ctcgggagga caacgtgatc cggaaatatg 181 gagaccccgg gagcctcttc ggcttctcgc tggccatgca ctggcaactg cagcccgagg 241 acaagcggct gttgctcgtg ggggccccgc gggcagaagc gcttccactg cagagagcca 301 acagaacggg agggctgtac agctgcgaca tcaccgcccg ggggccatgc acgcggatcg 361 agtttgataa cgatgctgac cccacgtcag aaagcaagga agatcagtgg atgggggtca 421 ccgtccagag ccaaggtcca gggggcaagg tcgtgacatg tgctcaccga tatgaaaaaa 481 ggcagcatgt taatacgaag caggaatccc gagacatctt tgggcggtgt tatgtcctga 541 gtcagaatct caggattgaa gacgatatgg atgggggaga ttggagcttt tgtgatgggc 601 gattgagagg ccatgagaaa tttggctctt gccagcaagg tgtagcagct acttttacta 661 aagactttca ttacattgta tttggagccc cgggtactta taactggaaa gggattgttc 721 gtgtagagca aaagaataac actttttttg acatgaacat ctttgaagat gggccttatg 781 aagttggtgg agagactgag catgatgaaa gtctcgttcc tgttcctgct aacagttact 841 taggtttttc tttggactca gggaaaggta ttgtttctaa agatgagatc acttttgtat 901 ctggtgctcc cagagccaat cacagtggag ccgtggtttt gctgaagaga gacatgaagt 961 ctgcacatct cctccctgag cacatattcg atggagaagg tctggcctct tcatttggct 1021 atgatgtggc ggtggtggac ctcaacaagg atgggtggca agatatagtt attggagccc 1081 cacagtattt tgatagagat ggagaagttg gaggtgcagt gtatgtctac atgaaccagc 1141 aaggcagatg gaataatgtg aagccaattc gtcttaatgg aaccaaagat tctatgtttg 1201 gcattacagt aaaaaatatt ggagatatta atcaagatgg ctacccagat attgcagttg 1261 gagctccgta tgatgacttg ggaaaggttt ttatctatca tggatctgca aatggaataa 1321 ataccaaacc aacacaggtt ctcaagggta tatcacctta ttttggatat tcaattgctg 1381 gaaacatgga ccttgatcga aattcctacc ctgatgttgc tgttggttcc ctctcagatt 1441 cagtaactat tttcagatcc cggcctgtga ttaatattca gaaaaccatc acagtaactc 1501 ctaacagaat tgacctccgc cagaaaacag cgtgtggggc gcctagtggg atatgcctcc 1561 aggttaaatc ctgttttgaa tatactgcta accccgctgg ttataatcct tcaatatcaa 1621 ttgtgggcac acttgaagct gaaaaagaaa gaagaaaatc tgggctatcc tcaagagttc 1681 agtttcgaaa ccaaggttct gagcccaaat atactcaaga actaactctg aagaggcaga 1741 aacagaaagt gtgcatggag gaaaccctgt ggctacagga taatatcaga gataaactgc 1801 gtcccattcc cataactgcc tcagtggaga tccaagagcc aagctctcgt aggcgagtga 1861 attcacttcc agaagttctt ccaattctga attcagatga acccaagaca gctcatattg 1921 atgttcactt cttaaaagag ggatgtggag acgacaatgt atgtaacagc aaccttaaac 1981 tagaatataa attttgcacc cgagaaggaa atcaagacaa attttcttat ttaccaattc 2041 aaaaaggtgt accagaacta gttctaaaag atcagaagga tattgcttta gaaataacag 2101 tgacaaacag cccttccaac ccaaggaatc ccacaaaaga tggcgatgat gcccatgagg 2161 ctaaactgat tgcaacgttt ccagacactt taacctattc tgcatataga gaactgaggg 2221 ctttccctga gaaacagttg agttgtgttg ccaaccagaa tggctcgcaa gctgactgtg 2281 agctcggaaa tccttttaaa agaaattcaa atgtcacttt ttatttggtt ttaagtacaa 2341 ctgaagtcac ctttgacacc ccagatctgg atattaatct gaagttagaa acaacaagca 2401 atcaagataa tttggctcca attacagcta aagcaaaagt ggttattgaa ctgcttttat 2461 cggtctcggg agttgctaaa ccttcccagg tgtattttgg aggtacagtt gttggcgagc 2521 aagctatgaa atctgaagat gaagtgggaa gtttaataga gtatgaattc agggtaataa 2581 acttaggtaa acctcttaca aacctcggca cagcaacctt gaacattcag tggccaaaag 2641 aaattagcaa tgggaaatgg ttgctttatt tggtgaaagt agaatccaaa ggattggaaa 2701 aggtaacttg tgagccacaa aaggagataa actccctgaa cctaacggag tctcacaact 2761 caagaaagaa acgggaaatt actgaaaaac agatagatga taacagaaaa ttttctttat 2821 ttgctgaaag aaaataccag actcttaact gtagcgtgaa cgtgaactgt gtgaacatca 2881 gatgcccgct gcgggggctg gacagcaagg cgtctcttat tttgcgctcg aggttatgga 2941 acagcaCATT TCTAGAGGAA TATTCCAAAC TGAACTACTT GGACATTCTC ATGCGAGCCT 3001 TCATTGATGT GACTGCTGCT GCCGAAAATA TCAGGCTGCC AAATGCAGGC ACTCAGGTTC 3061 GAGTGACTGT GTTTCCCTCA AAGACTGTAG CTCAGTATTC GGGAGTACCT TGGTGGATCA 3121 TCCTAGTGGC TATTCTCGCT GGGATCTTGA TGCTTGCTTT ATTAGTGTTT ATACTATGGA 3181 AGTGTGGTTT CTTCAAGAGA AATAAGAAAG ATCATTATGA TGCCACATAT CACAAGGCTG 3241 AGATCCATGC TCAGCCATCT GATAAAGAGA GGCTTACTTC TGATGCATTG CCAACTTTCT 3301 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 3361 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 3421 GTGGAAAGGA CGATGGTCTT CCTACACCCC TGGCGGGACG CGTTAAGTCg acaatcaacc 3481 tctggattac aaaatttgtg aaagatt