Construct: ORF TRCN0000479989
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009935.1_s317c1
- Derived from:
- ccsbBroadEn_12026
- DNA Barcode:
- GTGTTCTATATCCATCTATCCCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FGFRL1 (53834)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479989
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 53834 | FGFRL1 | fibroblast growth factor re... | NM_001004356.3 | 69.3% | 69.2% | 1_462del;1085C>A |
| 2 | human | 53834 | FGFRL1 | fibroblast growth factor re... | NM_001004358.1 | 69.3% | 69.2% | 1_462del;1085C>A |
| 3 | human | 53834 | FGFRL1 | fibroblast growth factor re... | NM_001370296.1 | 69.3% | 69.2% | 1_462del;1085C>A |
| 4 | human | 53834 | FGFRL1 | fibroblast growth factor re... | NM_021923.3 | 69.3% | 69.2% | 1_462del;1085C>A |
| 5 | human | 53834 | FGFRL1 | fibroblast growth factor re... | XM_024454093.1 | 69.3% | 69.2% | 1_462del;1085C>A |
| 6 | human | 53834 | FGFRL1 | fibroblast growth factor re... | XM_024454092.1 | 66.3% | 66.2% | 1_531del;1154C>A |
| 7 | mouse | 116701 | Fgfrl1 | fibroblast growth factor re... | NM_001164259.1 | 65.6% | 64.6% | (many diffs) |
| 8 | mouse | 116701 | Fgfrl1 | fibroblast growth factor re... | NM_054071.2 | 54.5% | 53.9% | (many diffs) |
| 9 | mouse | 116701 | Fgfrl1 | fibroblast growth factor re... | XM_017320618.1 | 54.5% | 53.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1116
- ORF length:
- 1050
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gcgccgggtg atcgcacggc ccgtgggtag ctccgtgcgg ctcaagtgcg 121 tggccagcgg gcaccctcgg cccgacatca cgtggatgaa ggacgaccag gccttgacgc 181 gcccagaggc cgctgagccc aggaagaaga agtggacact gagcctgaag aacctgcggc 241 cggaggacag cggcaaatac acctgccgcg tgtcgaaccg cgcgggcgcc atcaacgcca 301 cctacaaggt ggatgtgatc cagcggaccc gttccaagcc cgtgctcaca ggcacgcacc 361 ccgtgaacac gacggtggac ttcgggggga ccacgtcctt ccagtgcaag gtgcgcagcg 421 acgtgaagcc ggtgatccag tggctgaagc gcgtggagta cggcgccgag ggccgccaca 481 actccaccat cgatgtgggc ggccagaagt ttgtggtgct gcccacgggt gacgtgtggt 541 cgcggcccga cggctcctac ctcaataagc tgctcatcac ccgtgcccgc caggacgatg 601 cgggcatgta catctgcctt ggcgccaaca ccatgggcta cagcttccgc agcgccttcc 661 tcaccgtgct gccagaccca aaaccgcaag ggccacctgt ggcctcctcg tcctcggcca 721 ctagcctgcc gtggcccgtg gtcatcggca tcccagccgg cgctgtcttc atcctgggca 781 ccctgctcct gtggctttgc caggcccaga agaagccgtg cacccccgcg cctgcccctc 841 ccctgcctgg gcaccgcccg ccggggacgg cccgcgaccg cagcggagac aaggaccttc 901 cctcgttggc cgccctcagc gctggccctg gtgtggggct gtgtgaggag catgggTCTC 961 CGGCAGCCCC CCAGCACTTA CTGGGCCCAG GCCCAGTTGC TGGCCCTAAG TTGTACCCCA 1021 AACTCTACAC AGACATCCAC ACACACACAC ACACACACTC TCACACACAC TCACACGTGG 1081 AGGGCAAGGT CCACCAGCAC ATCCACTATC AGTGCTACCC AACTTTCTTG TACAAAGTGG 1141 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1201 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1261 AGTGTTCTAT ATCCATCTAT CCCTCACGCG TTAAGTCgac aatcaacctc tggattacaa 1321 aatttgtgaa agatt