Construct: ORF TRCN0000480002
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011707.1_s317c1
- Derived from:
- ccsbBroadEn_07932
- DNA Barcode:
- TGCTCAGTAATGGCGAGCAGCACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARHGAP8 (23779)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480002
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23779 | ARHGAP8 | Rho GTPase activating prote... | NM_181335.2 | 99.6% | 99.7% | (many diffs) |
| 2 | human | 23779 | ARHGAP8 | Rho GTPase activating prote... | NM_001017526.1 | 93% | 93.1% | (many diffs) |
| 3 | human | 553158 | PRR5-ARHGAP8 | PRR5-ARHGAP8 readthrough | NM_181334.5 | 76.5% | 76.5% | (many diffs) |
| 4 | human | 23779 | ARHGAP8 | Rho GTPase activating prote... | NM_001198726.1 | 70.2% | 68.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1368
- ORF length:
- 1299
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctggccag gatcctgcgc tgagcacgag tcacccgttc tacgacgtgg 121 ccagacatgg cattctgcag gtggcagggg atgaccgctt tggaagacgt gttgtcacgt 181 tcagctgctg ccggatgccg ccctcccacg agctggacca ccagcggctg ctggagtatt 241 tgaagtacac actggaccaa tacgttgaga acgattatac catcgtctat ttccactacg 301 ggctgaacag ccggaacaag ccttccctgg gctggctcca gagcgcatac aaggagttcg 361 ataggaagta caagaagaac ttgaaggccc tctacgtggt gcaccccacc agctttatca 421 aggtcctgtg gaacatcttg aagcccctca tcagtcacaa gtttgggaag aaagtcatct 481 atttcaacta cctgagtgag ctccacgaac accttaaata cgaccagctg gtcatccctc 541 ccgaagtttt gcggtacgat gagaagctcc agagcctgca cgagggccgg acgccgcctc 601 ccaccaagac accaccgccg cggcccccgc tgcccacaca gcagtttggc gtcagtctgc 661 aatacctcaa agacaaaaat caaggcgaac tcatcccccc tgtgctgagg ttcacagtga 721 cgtacctgag agagaaaggc ctgcgcaccg agggcctgtt ccggagatcc gccagcgtgc 781 agaccgtccg cgagatccag aggctctaca accaagggaa gcccgtgaac tttgacgact 841 acggggacat tcacatccct gccgtgatcc tgaagacctt cctgcgagag ctgccccagc 901 cgcttctgac cttccaggcc tacgagcaga ttcttgggat cacctgtgtg gagagcagcc 961 tgcgtgtcac tcgctgccgc cagatcttac ggagcctccc agagcacaac tacgtcgtcc 1021 tccgctaccT CATGGGCTTC CTGCATGCGG TGTCCCGGGA GAGCATCTTC AACAAAATGA 1081 ACAGCTCTAA CCTGGCCTGT GTCTTCGGGC TGAATTTGAT CTGGCCATCC CAGGGGGTCT 1141 CCTCCCTGAG TGCCCTTGTG CCCCTGAACA TGTTCACTGA ACTGCTGATC GAGTACTATG 1201 AAAAGATCTT CAGCACCCCG GAGGCACCTG GGGAGCACGG CCTGGCACCA TGGGAACAGG 1261 GGAGCAGGGC AGCCCCTTTG CAGGAGGCTG TGCCACGGAC ACAAGCCACG GGCCTCACCA 1321 AGCCTACCCT ACCTCCGAGT CCCCTGATGG CAGCCAGAAG ACGTCTCTTG CCAACTTTCT 1381 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1441 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1501 GTGGAAAGGA CGATGCTCAG TAATGGCGAG CAGCACTACG CGTTAAGTCg acaatcaacc 1561 tctggattac aaaatttgtg aaagatt