Construct: ORF TRCN0000480009

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003697.1_s317c1
Derived from:
ccsbBroadEn_07743
DNA Barcode:
GTATGCACGTCATATTAGGGCATC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TPPP (11076)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480009
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 11076 TPPP tubulin polymerization prom... NM_007030.3 99.6% 100% 114T>C;597A>G
2 human 11076 TPPP tubulin polymerization prom... XM_005248237.3 99.6% 100% 114T>C;597A>G
3 human 11076 TPPP tubulin polymerization prom... XM_024454346.1 99.6% 100% 114T>C;597A>G
4 human 11076 TPPP tubulin polymerization prom... XM_017008993.1 76.3% 76.5% 1_201del;315T>C;798A>G
5 mouse 72948 Tppp tubulin polymerization prom... NM_182839.2 85.3% 91.8% (many diffs)
6 mouse 72948 Tppp tubulin polymerization prom... XM_006517409.3 85.3% 91.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
726
ORF length:
657
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggctgacaag gccaagcctg ccaaagctgc caacaggacg ccccccaagt
121ccccggggga cccctcgaag gaccgggcag ccaagaggct gtcgctggaa tcggagggtg
181ccggtgaggg ggcagccgca tcccctgagc tcagtgccct ggaggaggcc ttccggcgct
241ttgccgtgca cggggacgcc agggccaccg ggagggagat gcacggcaag aactggtcga
301agctgtgcaa ggactgccag gtgatcgacg gcaggaacgt gaccgtcact gacgtggaca
361tcgtcTTCAG CAAGATCAAA GGGAAGTCTT GCCGGACCAT CACCTTTGAG CAGTTCCAGG
421AGGCGCTGGA GGAGCTCGCC AAGAAGCGAT TCAAAGACAA GAGCAGCGAG GAGGCCGTTC
481GCGAGGTGCA CAGGCTCATC GAGGGCAAGG CGCCCATCAT CTCAGGGGTG ACGAAAGCCA
541TCTCGTCGCC CACAGTGTCG AGGCTCACGG ACACCACCAA GTTCACGGGC TCCCACAAGG
601AGCGCTTCGA CCCCTCTGGC AAGGGCAAGG GCAAGGCTGG CCGCGTGGAT CTGGTGGACG
661AGTCGGGCTA TGTGTCCGGC TACAAGCACG CAGGCACCTA CGACCAGAAG GTGCAAGGGG
721GCAAGTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC
781TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT
841CTTGGCTTTA TATATCTTGT GGAAAGGACG AGTATGCACG TCATATTAGG GCATCACGCG
901TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt

Download FASTA (ORF) (Full)