Construct: ORF TRCN0000480016
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016562.1_s317c1
- Derived from:
- ccsbBroadEn_13554
- DNA Barcode:
- GTGACAGTGACTACGTGACCGTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C5orf51 (285636)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480016
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285636 | C5orf51 | chromosome 5 open reading f... | XM_005248289.4 | 81.8% | 76.8% | (many diffs) |
| 2 | human | 285636 | C5orf51 | chromosome 5 open reading f... | NM_175921.6 | 46.2% | 45.4% | (many diffs) |
| 3 | human | 285636 | C5orf51 | chromosome 5 open reading f... | XM_011514032.2 | 22.1% | 21% | (many diffs) |
| 4 | human | 285636 | C5orf51 | chromosome 5 open reading f... | XR_001742054.1 | 7.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 525
- ORF length:
- 456
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggccgca gtctctagtg tggtgagacg agtggaagag ctcggggatc 121 tggcTCAGGC CCACATACAG CAACTTAGCG AAGCTGCCGG TGAAGATGAT CACTTTTTAA 181 TTCGGGCCTC TGCAGCATTA GAAAAATTGA AACTCCTGTG TGGAGAAGAG AAAGAATGTT 241 CAAATCCATC AAATCTTCTA GAACTTTACA CACAGGCTAT TTTGGACATG ACATATTTTG 301 AGGAGAACAA GCTAGTAGAT GAAGATTTTC CTGAAGACTC TTCTTCACAG AAAGTAAAAG 361 AGCTGATTAG TTTTCTTTCA GAACCAGAAA TTTTAGTAAA GGAAAATAAT ATGCATCCAA 421 AACACTGCAA TTTGCTTGGG GATGAGCTAC TGGAATGTCT CTCCCATTTC CCGCTGTGGC 481 TGAGCCAAAC TTCATTACTT TCCTTAGGCC CTCTGCCCCT CCCCTTGCCA ACTTTCTTGT 541 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 601 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 661 GAAAGGACGA GTGACAGTGA CTACGTGACC GTGGACGCGT TAAGTCgaca atcaacctct 721 ggattacaaa atttgtgaaa gatt