Construct: ORF TRCN0000480017
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014879.1_s317c1
- Derived from:
- ccsbBroadEn_07415
- DNA Barcode:
- GTCCCGGCGGGTACTAGCGGGCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ECEL1 (9427)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480017
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9427 | ECEL1 | endothelin converting enzym... | NM_004826.4 | 99.9% | 99.8% | 982C>T |
| 2 | human | 9427 | ECEL1 | endothelin converting enzym... | NM_001290787.2 | 99.6% | 99.6% | 982C>T;1679_1680insGTCCAC |
| 3 | mouse | 13599 | Ecel1 | endothelin converting enzym... | NM_001277925.1 | 88.6% | 94.7% | (many diffs) |
| 4 | mouse | 13599 | Ecel1 | endothelin converting enzym... | NM_021306.3 | 88.6% | 94.7% | (many diffs) |
| 5 | mouse | 13599 | Ecel1 | endothelin converting enzym... | XM_017314898.1 | 88.6% | 94.7% | (many diffs) |
| 6 | mouse | 13599 | Ecel1 | endothelin converting enzym... | XM_017314902.1 | 88.6% | 94.7% | (many diffs) |
| 7 | mouse | 13599 | Ecel1 | endothelin converting enzym... | XM_017314903.1 | 88.6% | 94.7% | (many diffs) |
| 8 | mouse | 13599 | Ecel1 | endothelin converting enzym... | XR_001780293.1 | 41.6% | (many diffs) | |
| 9 | mouse | 13599 | Ecel1 | endothelin converting enzym... | XR_001780275.1 | 40.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2391
- ORF length:
- 2325
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gcccccgtat tcgctgacgg cgcactacga tgagttccaa gaggtcaagt 121 acgtgagccg ctgcggcgcg gggggcgcgc gcggggcctc cctgcccccg ggcttcccgt 181 tgggcgctgc gcgcagcgcc accggggccc ggtccgggct gccgcgctgg aaccggcgcg 241 aggtgtgcct gctgtcgggg ctggtgttcg ccgccggcct ctgcgccatt ctggcggcta 301 tgctggccct caagtacctg ggcccggtcg cggccggcgg cggcgcctgt cccgagggct 361 gccctgagcg caaggccttc gcgcgcgccg ctcgcttcct ggccgccaac ctggacgcca 421 gcatcgaccc atgccaggac ttctactcgt tcgcctgcgg cggttggctg cggcgccacg 481 ccatccccga cgacaagctc acctatggca ccatcgcggc catcggcgag caaaacgagg 541 agcgcctacg gcgcctgctg gcgcggcccg ggggtgggcc tggcggcgcg gcccagcgca 601 aggtgcgcgc cttcttccgc tcgtgcctcg acatgcgcga gatcgagcga ctgggcccgc 661 gacccatgct agaggtcatc gaggactgcg ggggctggga cctgggcggc gcggaggagc 721 gtccgggggt cgcggcgcga tgggacctca accggctgct gtacaaggcg cagggcgtgt 781 acagcgccgc cgcgctcttc tcgctcacgg tcagcctgga cgacaggaac tcctcgcgct 841 acgtcatccg cattgaccag gatgggctca ccctgccaga gaggaccctg tacctcgctc 901 aggatgagga cagtgagaag atcctggcag catacagggt gttcatggag cgagtgctca 961 gcctcctggg tgcagacgct gtggaacaga aggcccaaga gatcctgcaa gtggagcagc 1021 agctggccaa catcactgtg tcagagtatg acgacctacg gcgagatgtc agctccatgt 1081 acaacaaggt gacgctgggg cagctgcaga agatcacccc ccacttgcgg tggaagtggc 1141 tgctagacca gatcttccag gaggacttct cagaggaaga ggaggtggtg ctgctggcga 1201 cagactacat gcagcaggtg tcgcagctca tccgctccac accccaccgg gtcctgcaca 1261 actacctggt gtggcgcgtg gtggtggtcc tgagtgaaca cctgtccccg ccattccgtg 1321 aggcactgca cgagctggca caggagatgg agggcagcga caagccacag gagctggccc 1381 gggtctgctt gggccaggcc aatcgccact ttggcatggc gcttggcgcc ctctttgtac 1441 atgagcactt ctcagctgcc agcaaagcca aggtgcagca gctagtggaa gacatcaagt 1501 acatcctggg ccagcgcctg gaggagctgg actggatgga cgccgagacc agggctgctg 1561 ctcgggccaa gctccagtac atgatggtga tggtcggcta cccggacttc ctgctgaaac 1621 ccgatgctgt ggacaaggag tatgagtttg aggtccatga gaagacctac ttcaagaaca 1681 tcttgaacag catccgcttc agcatccagc tctcagttaa gaagattcgg caggaggtgg 1741 acaagtccac gtggctgctc cccccacagg cgctcaatgc ctactatcta cccaacaaga 1801 accagatggt gttccccgcg ggcatcctgc agcccaccct gtacgaccct gacttcccac 1861 agtctctcaa ctacgggggc atcggcacca tcattggaca tgagctgacc cacggctacg 1921 acgactgggg gggccagtat gaccgctcag ggaacctgct gcactggtgg acggaggcct 1981 cctacagccg cttccTGCGA AAGGCTGAGT GCATCGTCCG TCTCTATGAC AACTTCACTG 2041 TCTACAACCA GCGGGTGAAC GGGAAACACA CGCTTGGGGA GAACATCGCA GATATGGGCG 2101 GCCTCAAGCT GGCCTACCAC GCCTATCAGA AGTGGGTGCG GGAGCACGGC CCAGAGCACC 2161 CACTTCCCCG GCTCAAGTAC ACACATGACC AGCTCTTCTT CATTGCCTTT GCCCAGAACT 2221 GGTGCATCAA GCGGCGGTCG CAGTCCATCT ACCTGCAGGT GCTGACTGAC AAGCATGCCC 2281 CTGAGCACTA CAGGGTGCTG GGCAGTGTGT CCCAGTTTGA GGAGTTTGGC CGGGCTTTCC 2341 ACTGTCCCAA GGACTCACCC ATGAACCCTG CCCACAAGTG TTCCGTGTGG TGCCCAACTT 2401 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2461 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2521 CTTGTGGAAA GGACGAGTCC CGGCGGGTAC TAGCGGGCGC ACGCGTTAAG TCgacaatca 2581 acctctggat tacaaaattt gtgaaagatt