Construct: ORF TRCN0000480032

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019033.2_s317c1
Derived from:
ccsbBroadEn_14627
DNA Barcode:
GCTATGCCGAACCCCACGACAGTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EPHA4 (2043)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480032
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2043 EPHA4 EPH receptor A4 NM_001304536.1 100% 100%
2 human 2043 EPHA4 EPH receptor A4 NM_004438.5 100% 100%
3 human 2043 EPHA4 EPH receptor A4 NM_001363748.2 96.2% 96.2% 2847_2848ins111
4 human 2043 EPHA4 EPH receptor A4 NM_001304537.2 94.7% 94.6% 0_1ins152;2T>G;5_5delAinsGG
5 mouse 13838 Epha4 Eph receptor A4 NM_007936.3 90.6% 98.5% (many diffs)
6 mouse 13838 Epha4 Eph receptor A4 XM_006496407.2 87.2% 94.9% (many diffs)
7 mouse 13838 Epha4 Eph receptor A4 XM_006496408.1 85.7% 93.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3027
ORF length:
2958
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggctgggatt ttctatttcg ccctattttc gtgtctcttc gggatttgcg
121acgctgtcac aggttccagg gtataccccg cgaatgaagt taccttattg gattccagat
181ctgttcaggg agaacttggg tggatagcaa gccctctgga aggagggtgg gaggaagtga
241gtatcatgga tgaaaaaaat acaccaatcc gaacctacca agtgtgcaat gtgatggaac
301ccagccagaa taactggcta cgaactgatt ggatcacccg agaaggggct cagagggtgt
361atattgagat taaattcacc ttgagggact gcaatagtct tccgggcgtc atggggactt
421gcaaggagac gtttaacctg tactactatg aatcagacaa cgacaaagag cgtttcatca
481gagagaacca gtttgtcaaa attgacacca ttgctgctga tgagagcttc acccaagtgg
541acattggtga cagaatcatg aagctgaaca ccgagatccg ggatgtaggg ccattaagca
601aaaaggggtt ttacctggct tttcaggatg tgggggcctg catcgccctg gtatcagtcc
661gtgtgttcta taaaaagtgt ccactcacag tccgcaatct ggcccagttt cctgacacca
721tcacaggggc tgatacgtct tccctggtgg aagttcgagg ctcctgtgtc aacaactcag
781aagagaaaga tgtgccaaaa atgtactgtg gggcagatgg tgaatggctg gtacccattg
841gcaactgcct atgcaacgct gggcatgagg agcggagcgg agaatgccaa gcttgcaaaa
901ttggatatta caaggctctc tccacggatg ccacctgtgc caagtgccca ccccacagct
961actctgtctg ggaaggagcc acctcgtgca cctgtgaccg aggctttttc agagctgaca
1021acgatgctgc ctctatgccc tgcacccgtc caccatctgc tcccctgaac ttgatttcaa
1081atgtcaacga gacatctgtg aacttggaat ggagtagccc tcagaataca ggtggccgcc
1141aggacatttc ctataatgtg gtatgcaaga aatgtggagc tggtgacccc agcaagtgcc
1201gaccctgtgg aagtggggtc cactacaccc cacagcagaa tggcttgaag accaccaaag
1261tctccatcac tgacctccta gctcatacca attacacctt tgaaatctgg gctgtgaatg
1321gagtgtccaa atataaccct aacccagacc aatcagtttc tgtcactgtg accaccaacc
1381aagcagcacc atcatccatt gctttggtcc aggctaaaga agtcacaaga tacagtgtgg
1441cactggcttg gctggaacca gatcggccca atggggtaat cctggaatat gaagtcaagt
1501attatgagaa ggatcagaat gagcgaagct atcgtatagt tcggacagct gccaggaaca
1561cagatatcaa aggcctgaac cctctcactt cctatgtttt ccacgtgcga gccaggacag
1621cagctggcta tggagacttc agtgagccct tggaggttac aaccaacaca gtgccttccc
1681ggatcattgg agatggggct aactccacag tccttctggt ctctgtctcg ggcagtgtgg
1741tgctggtggt aattctcatt gcagcttttg tcatcagccg gagacggagt aaatacagta
1801aagccaaaca agaagcggat gaagagaaac atttgaatca aggtgtaaga acatatgtgg
1861acccctttac gtacgaagat cccaaccaag cagtgcgaga gtttgccaaa gaaattgacg
1921catcctgcat taagattgaa aaagttatag gagttggtga atttggtgag gtatgcagtg
1981ggcgtctcaa agtgcctggc aagagagaga tctgtgtggc tatcaagact ctgaaagctg
2041gttatacaga caaacagagg agagacttcc tgagtgaggc cagcatcatg ggacagtttg
2101accatccgaa catcattcac ttggaaggcg tggtcactaa atgtaaacca gtaatgatca
2161taacagagta catggagaat ggctccttgg atgcattcct caggaaaaat gatggcagat
2221ttacagtcat tcagctggtg ggcatgcttc gtggcattgg gtctgggatg aagtatttat
2281ctgatatgag ctatgtgcat cgtgatctgg ccgcacggaa catcctggtg aacagcaact
2341tggtctgcaa agtgtctgat tttggcatgt cccgagtgct tgaggatgat ccggaagcag
2401cttacaccac caggggtggc aagattccta tccggtggac tgcgccagaa gcaattgcct
2461atcgtaaatt cacatcagca agtgatgtat ggagctatgg aatcgttatg tgggaagtga
2521tgtcgtacgg ggagaggccc tattgggata tgtccaatca agatgtgatt aaagccattg
2581aggaaggcta tcggttaccc cctccaatgg actgccccat tgcgctccac cagctgatgc
2641tagactgctg gcagaaggag aggagcgaca ggcctaaatt tgggcagatt gtcaacatgt
2701tggacaaacT CATCCGCAAC CCCAACAGCT TGAAGAGGAC AGGGACGGAG AGCTCCAGAC
2761CTAACACTGC CTTGTTGGAT CCAAGCTCCC CTGAATTCTC TGCTGTGGTA TCAGTGGGCG
2821ATTGGCTCCA GGCCATTAAA ATGGACCGGT ATAAGGATAA CTTCACAGCT GCTGGTTATA
2881CCACACTAGA GGCTGTGGTG CACGTGAACC AGGAGGACCT GGCAAGAATT GGTATCACAG
2941CCATCACGCA CCAGAATAAG ATTTTGAGCA GTGTCCAGGC AATGCGAACC CAAATGCAGC
3001AGATGCACGG CAGAATGGTT CCCGTCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA
3061AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
3121TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG CTATGCCGAA
3181CCCCACGACA GTTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
3241att

Download FASTA (ORF) (Full)