Construct: ORF TRCN0000480032
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019033.2_s317c1
- Derived from:
- ccsbBroadEn_14627
- DNA Barcode:
- GCTATGCCGAACCCCACGACAGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPHA4 (2043)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480032
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2043 | EPHA4 | EPH receptor A4 | NM_001304536.1 | 100% | 100% | |
| 2 | human | 2043 | EPHA4 | EPH receptor A4 | NM_004438.5 | 100% | 100% | |
| 3 | human | 2043 | EPHA4 | EPH receptor A4 | NM_001363748.2 | 96.2% | 96.2% | 2847_2848ins111 |
| 4 | human | 2043 | EPHA4 | EPH receptor A4 | NM_001304537.2 | 94.7% | 94.6% | 0_1ins152;2T>G;5_5delAinsGG |
| 5 | mouse | 13838 | Epha4 | Eph receptor A4 | NM_007936.3 | 90.6% | 98.5% | (many diffs) |
| 6 | mouse | 13838 | Epha4 | Eph receptor A4 | XM_006496407.2 | 87.2% | 94.9% | (many diffs) |
| 7 | mouse | 13838 | Epha4 | Eph receptor A4 | XM_006496408.1 | 85.7% | 93.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3027
- ORF length:
- 2958
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctgggatt ttctatttcg ccctattttc gtgtctcttc gggatttgcg 121 acgctgtcac aggttccagg gtataccccg cgaatgaagt taccttattg gattccagat 181 ctgttcaggg agaacttggg tggatagcaa gccctctgga aggagggtgg gaggaagtga 241 gtatcatgga tgaaaaaaat acaccaatcc gaacctacca agtgtgcaat gtgatggaac 301 ccagccagaa taactggcta cgaactgatt ggatcacccg agaaggggct cagagggtgt 361 atattgagat taaattcacc ttgagggact gcaatagtct tccgggcgtc atggggactt 421 gcaaggagac gtttaacctg tactactatg aatcagacaa cgacaaagag cgtttcatca 481 gagagaacca gtttgtcaaa attgacacca ttgctgctga tgagagcttc acccaagtgg 541 acattggtga cagaatcatg aagctgaaca ccgagatccg ggatgtaggg ccattaagca 601 aaaaggggtt ttacctggct tttcaggatg tgggggcctg catcgccctg gtatcagtcc 661 gtgtgttcta taaaaagtgt ccactcacag tccgcaatct ggcccagttt cctgacacca 721 tcacaggggc tgatacgtct tccctggtgg aagttcgagg ctcctgtgtc aacaactcag 781 aagagaaaga tgtgccaaaa atgtactgtg gggcagatgg tgaatggctg gtacccattg 841 gcaactgcct atgcaacgct gggcatgagg agcggagcgg agaatgccaa gcttgcaaaa 901 ttggatatta caaggctctc tccacggatg ccacctgtgc caagtgccca ccccacagct 961 actctgtctg ggaaggagcc acctcgtgca cctgtgaccg aggctttttc agagctgaca 1021 acgatgctgc ctctatgccc tgcacccgtc caccatctgc tcccctgaac ttgatttcaa 1081 atgtcaacga gacatctgtg aacttggaat ggagtagccc tcagaataca ggtggccgcc 1141 aggacatttc ctataatgtg gtatgcaaga aatgtggagc tggtgacccc agcaagtgcc 1201 gaccctgtgg aagtggggtc cactacaccc cacagcagaa tggcttgaag accaccaaag 1261 tctccatcac tgacctccta gctcatacca attacacctt tgaaatctgg gctgtgaatg 1321 gagtgtccaa atataaccct aacccagacc aatcagtttc tgtcactgtg accaccaacc 1381 aagcagcacc atcatccatt gctttggtcc aggctaaaga agtcacaaga tacagtgtgg 1441 cactggcttg gctggaacca gatcggccca atggggtaat cctggaatat gaagtcaagt 1501 attatgagaa ggatcagaat gagcgaagct atcgtatagt tcggacagct gccaggaaca 1561 cagatatcaa aggcctgaac cctctcactt cctatgtttt ccacgtgcga gccaggacag 1621 cagctggcta tggagacttc agtgagccct tggaggttac aaccaacaca gtgccttccc 1681 ggatcattgg agatggggct aactccacag tccttctggt ctctgtctcg ggcagtgtgg 1741 tgctggtggt aattctcatt gcagcttttg tcatcagccg gagacggagt aaatacagta 1801 aagccaaaca agaagcggat gaagagaaac atttgaatca aggtgtaaga acatatgtgg 1861 acccctttac gtacgaagat cccaaccaag cagtgcgaga gtttgccaaa gaaattgacg 1921 catcctgcat taagattgaa aaagttatag gagttggtga atttggtgag gtatgcagtg 1981 ggcgtctcaa agtgcctggc aagagagaga tctgtgtggc tatcaagact ctgaaagctg 2041 gttatacaga caaacagagg agagacttcc tgagtgaggc cagcatcatg ggacagtttg 2101 accatccgaa catcattcac ttggaaggcg tggtcactaa atgtaaacca gtaatgatca 2161 taacagagta catggagaat ggctccttgg atgcattcct caggaaaaat gatggcagat 2221 ttacagtcat tcagctggtg ggcatgcttc gtggcattgg gtctgggatg aagtatttat 2281 ctgatatgag ctatgtgcat cgtgatctgg ccgcacggaa catcctggtg aacagcaact 2341 tggtctgcaa agtgtctgat tttggcatgt cccgagtgct tgaggatgat ccggaagcag 2401 cttacaccac caggggtggc aagattccta tccggtggac tgcgccagaa gcaattgcct 2461 atcgtaaatt cacatcagca agtgatgtat ggagctatgg aatcgttatg tgggaagtga 2521 tgtcgtacgg ggagaggccc tattgggata tgtccaatca agatgtgatt aaagccattg 2581 aggaaggcta tcggttaccc cctccaatgg actgccccat tgcgctccac cagctgatgc 2641 tagactgctg gcagaaggag aggagcgaca ggcctaaatt tgggcagatt gtcaacatgt 2701 tggacaaacT CATCCGCAAC CCCAACAGCT TGAAGAGGAC AGGGACGGAG AGCTCCAGAC 2761 CTAACACTGC CTTGTTGGAT CCAAGCTCCC CTGAATTCTC TGCTGTGGTA TCAGTGGGCG 2821 ATTGGCTCCA GGCCATTAAA ATGGACCGGT ATAAGGATAA CTTCACAGCT GCTGGTTATA 2881 CCACACTAGA GGCTGTGGTG CACGTGAACC AGGAGGACCT GGCAAGAATT GGTATCACAG 2941 CCATCACGCA CCAGAATAAG ATTTTGAGCA GTGTCCAGGC AATGCGAACC CAAATGCAGC 3001 AGATGCACGG CAGAATGGTT CCCGTCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 3061 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 3121 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG CTATGCCGAA 3181 CCCCACGACA GTTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 3241 att