Construct: ORF TRCN0000480039
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011047.1_s317c1
- Derived from:
- ccsbBroadEn_02274
- DNA Barcode:
- TTGTAAAATTTGTTCCCGAGAGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HS3ST2 (9956)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480039
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9956 | HS3ST2 | heparan sulfate-glucosamine... | NM_006043.2 | 100% | 100% | |
| 2 | human | 9956 | HS3ST2 | heparan sulfate-glucosamine... | XM_011546001.3 | 51.6% | 44.3% | (many diffs) |
| 3 | human | 9956 | HS3ST2 | heparan sulfate-glucosamine... | XM_011546002.3 | 49% | 45.1% | (many diffs) |
| 4 | mouse | 195646 | Hs3st2 | heparan sulfate (glucosamin... | NM_001081327.1 | 89.3% | 96.1% | (many diffs) |
| 5 | mouse | 195646 | Hs3st2 | heparan sulfate (glucosamin... | XM_006507464.1 | 80.9% | 84.9% | (many diffs) |
| 6 | mouse | 195646 | Hs3st2 | heparan sulfate (glucosamin... | XM_006507465.1 | 80.7% | 86.6% | (many diffs) |
| 7 | mouse | 195646 | Hs3st2 | heparan sulfate (glucosamin... | XM_006507467.1 | 50.8% | 54.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1170
- ORF length:
- 1101
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcctatagg gtcctgggcc gcgcggggcc acctcagccg cggagggcgc 121 gcaggctgct cttcgccttc acgctctcgc tctcctgcac ttacctgtgt tacagcttcc 181 tgtgctgctg cgacgacctg ggtcggagcc gcctcctcgg cgcgcctcgc tgcctccgcg 241 gccccagcgc gggcggccag aaacttctcc agaagtcccg cccctgtgat ccctccgggc 301 cgacgcccag cgagcccagc gctcccagcg cgcccgccgc cgccgtgccc gcccctcgcc 361 tctccggttc caaccactcc ggctcaccca agctgggtac caagcggttg ccccaagccc 421 tcattgtggg cgtgaagaag gggggcaccc gggccgtgct ggagtttatc cgagtacacc 481 cggacgtgcg ggccttgggc acggaacccc acttctttga caggaactac ggccgcgggc 541 tggattggta caggagcctg atgcccagga ccctcgagag ccagatcacg ctggagaaga 601 cgcccagcta ctttgtcact caagaggctc ctcgacgcat cttcaacatg tcccgagaca 661 ccaagctgat cgtggttgtg cggaaccctg tgacccgtgc catctctgat tacacgcaga 721 cactctccaa gaagcccgac atcccgacct ttgagggcct ctccttccgc aaccgcaccc 781 tgggccTGGT GGACGTGTCA TGGAACGCCA TCCGCATCGG CATGTACGTG CTGCACCTGG 841 AGAGCTGGCT GCAGTACTTC CCGCTAGCTC AGATTCACTT CGTCAGTGGC GAGCGACTCA 901 TCACTGACCC GGCCGGCGAG ATGGGGCGAG TCCAGGACTT CCTGGGCATT AAGAGATTCA 961 TCACGGACAA GCACTTCTAT TTCAACAAGA CCAAAGGATT CCCTTGCTTG AAAAAAACAG 1021 AATCGAGCCT CCTGCCTCGA TGCTTGGGCA AATCAAAAGG GAGAACTCAT GTACAGATTG 1081 ATCCTGAAGT GATAGACCAG CTCCGAGAAT TTTATAGACC GTATAATATC AAATTTTATG 1141 AAACCGTTGG GCAGGACTTC AGGTGGGAAT TGCCAACTTT CTTGTACAAA GTGGTTGATA 1201 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1261 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATTGTA 1321 AAATTTGTTC CCGAGAGCCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1381 tgaaagatt