Construct: ORF TRCN0000480082
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004382.1_s317c1
- Derived from:
- ccsbBroadEn_07876
- DNA Barcode:
- TACTTTCGGATAGACCTTCCAGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LARS2 (23395)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480082
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23395 | LARS2 | leucyl-tRNA synthetase 2, m... | NM_001368263.1 | 99.8% | 100% | (many diffs) |
| 2 | human | 23395 | LARS2 | leucyl-tRNA synthetase 2, m... | NM_015340.4 | 99.8% | 100% | (many diffs) |
| 3 | human | 23395 | LARS2 | leucyl-tRNA synthetase 2, m... | XM_011533554.2 | 90.7% | 87.7% | (many diffs) |
| 4 | human | 23395 | LARS2 | leucyl-tRNA synthetase 2, m... | XM_017006042.1 | 88.7% | 88.7% | (many diffs) |
| 5 | mouse | 102436 | Lars2 | leucyl-tRNA synthetase, mit... | NM_001348167.1 | 85% | 86.4% | (many diffs) |
| 6 | mouse | 102436 | Lars2 | leucyl-tRNA synthetase, mit... | NM_153168.3 | 85% | 86.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2775
- ORF length:
- 2709
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ttctgtttgg cagagattgg gtttttatgc ctctcttctg aaaagacagc 121 taaatggtgg gccagatgtc atcaagtggg aaaggagagt aattcccgga tgtaccagaa 181 gcatctacag tgccacggga aagtggacaa aagagtatac attgcagaca agaaaggatg 241 ttgagaaatg gtggcatcaa cgaataaaag aacaggcctc caaaatttca gaagctgata 301 aatcgaagcc aaaattttac gtgctttcca tgttccctta tccttctggt aagctgcaca 361 tgggccatgt gcgtgtctac accatcagcg acaccatagc acggttccag aagatgagag 421 ggatgcaggt catcaacccc atgggatggg atgcttttgg attgcctgct gaaaatgccg 481 cagtcgagag gaatctacat ccacaaagtt ggacacaaag taatattaaa cacatgagga 541 aacagcttga tcgtctgggc ctgtgtttca gctgggatag ggaaataact acgtgtttgc 601 cagattacta caagtggact cagtatctct ttattaaact gtatgaggct gggctggcct 661 atcaaaagga ggccctggtt aactgggacc cagtggatca aacagtgctt gccaatgagc 721 aggtggatga acatggctgt tcatggcgtt ctggagcaaa ggtggaacag aagtacctca 781 gacaatggtt tattaagaca accgcttatg caaaggccat gcaggacgcg ttggcagacc 841 ttccagaatg gtatggaata aaaggcatgc aagcccactg gattggggac tgtgtgggct 901 gccacctgga cttcacatta aaggttcatg ggcaagccac gggcgaaaag ctgactgcct 961 atacggccac ccctgaagcc atttatggca cctcccacgt ggccatctcg cccagccaca 1021 gactcctaca tgggcacagc tctctgaagg aagccttgag gatggccctt gtccctggca 1081 aagattgcct cacgcctgta atggctgtga acatgctcac ccagcaggag gtccctgtcg 1141 ttattttggc caaagctgac ttggaaggct ctctggattc aaaaatagga attcccagta 1201 ctagctcaga ggacaccatc ttagcccaaa ccctgggcct ggcctactct gaagtcattg 1261 aaactttgcc agatggcaca gagagactga gcagctctgc tgagttcaca ggtatgaccc 1321 ggcaggatgc ttttctagcc ctgactcaga aagcccgggg gaagagagtg ggtggagacg 1381 tgacaagtga taaactgaaa gactggctga tttcacggca gcggtactgg ggcacaccaa 1441 tccccattgt ccactgccca gtctgtggcc ccacacctgt gcccctggag gacttgcctg 1501 tgaccctgcc caacatcgca tctttcactg gcaagggagg ccccccactg gccatggctt 1561 cagagtgggt gaactgctcc tgcccaaggt gcaagggagc agccaagaga gagacagaca 1621 cgatggatac ctttgttgat tctgcttggt actacttcag atacactgac cctcataatc 1681 cacacagccc ttttaacaca gcagtggccg attactggat gcctgtggat ttgtacattg 1741 gagggaaaga acatgccgtc atgcacttgt tctatgcaag attctttagt catttttgcc 1801 atgatcaaaa aatggttaaa catagggagc cttttcataa gctgctggcc caaggcctta 1861 tcaaggggca gacattccgc ctaccatctg gacagtatct acagagagag gaagtggatc 1921 tcacaggttc cgttcctgtt catgcaaaaa cgaaagagaa gttagaggtg acgtgggaga 1981 agatgagtaa gtccaaacac aacggggtgg acccagagga agttgtggag cagtatggga 2041 tcgacacgat tcggctctac atcctttttg ctgcccctcc tgagaaggat atcttgtggg 2101 atgtgaaaac tgatgctctc cctggggtgc tgagatggca acaacgactg tggaccttga 2161 caactcggtt tattgaggcc agggcttctg ggaagtctcc ccagcctcag ctgctgagta 2221 acaaggagaa agccgaggcc aggaagctct gggagtacaa gaactccgtc atctctcagg 2281 tgaccaccca tttcacagag gacttctcac tgaattctgc aatttctcag ctgatgggac 2341 TCAGCAATGC CCTCTCGCAA GCCTCTCAGA GCGTCATTCT CCACAGCCCC GAGTTTGAGG 2401 ATGCTTTGTG TGCCCTGATG GTGATGGCTG CTCCACTGGC CCCTCATGTA ACCTCAGAGA 2461 TCTGGGCAGG CCTGGCGCTG GTGCCGAGGA AGCTCTGTGC CCACTACACT TGGGATGCCA 2521 GTGTGCTGCT CCAGGCATGG CCTGCTGTGG ACCCGGAGTT CCTGCAGCAG CCTGAGGTTG 2581 TCCAGATGGC AGTTCTGATC AACAATAAAG CTTGTGGCAA AATTCCTGTG CCCCAACAAG 2641 TTGCCCGGGA CCAGGACAAA GTCCACGAAT TTGTTCTTCA AAGCGAGCTG GGTGTCAGGC 2701 TTTTGCAAGG ACGAAGCATC AAGAAGTCCT TCCTTTCCCC GAGAACTGCC CTCATCAACT 2761 TCCTGGTGCA AGATTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2821 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2881 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TACTTTCGGA TAGACCTTCC 2941 AGAAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt