Construct: ORF TRCN0000480115
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017809.1_s317c1
- Derived from:
- ccsbBroadEn_10115
- DNA Barcode:
- ACTTTTGCCAATGGCGAATCCTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM102A (399665)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480115
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 399665 | FAM102A | family with sequence simila... | NM_203305.2 | 99.8% | 100% | 588T>C |
| 2 | human | 399665 | FAM102A | family with sequence simila... | NM_001035254.3 | 62.9% | 63% | 1_426del;1014T>C |
| 3 | mouse | 98952 | Fam102a | family with sequence simila... | NM_153560.4 | 53.3% | 58.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 792
- ORF length:
- 726
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt cctgctctct ggagatccct gcttcaagac gccaccatcg actgccaagt 121 ccatctccat cccaggccag gattcctccc tgcagctgac gtgtaagggt ggtgggacca 181 gcagtggggg cagcagcacc aactccctga ctgggtcccg gccccccaag gctcggccca 241 ctattctcag ctcagggctg ccagaggaac ccgaccagaa cctgtccagc cctgaggagg 301 tgttccactc tggccactcc cgcaactcca gctatgccag ccagcagtcc aagatctccg 361 gctacagcac agagcactcg cgctcctcca gcctcTCAGA CCTGACGCAC CGCCGCAACA 421 CGTCCACCAG CAGCAGCGCC TCTGGGGGCC TTGGCATGAC CGTGGAGGGC CCTGAGGGCA 481 GTGAGCGGGA GCACCGGCCC CCGGAGAAGC CGCCGCGGCC ACCCCGGCCC CTGCATCTGT 541 CCGATCGCTC TTTCAGGCGG AAGAAGGACT CGGTGGAGAG CCACCCGACC TGGGTGGACG 601 ACACGCGGAT CGATGCGGAT GCCATCGTGG AGAAGATCGT GCAGAGCCAG GACTTCACAG 661 ATGGCAGCAA CACCGAGGAC AGCAACCTCC GGCTGTTCGT GAGCCGCGAT GGCTCTGCCA 721 CGCTGAGCGG CATCCAGCTT GCCACCAGGG TCTCTTCTGG GGTCTACGAG CCAGTTGTGA 781 TTGAAAGCCA TTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 841 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 901 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAACT TTTGCCAATG GCGAATCCTC 961 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t