Construct: ORF TRCN0000480119
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015322.2_s317c1
- Derived from:
- ccsbBroadEn_16131
- DNA Barcode:
- GGTCCACTGTTAAACGCCGCTGGG
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MICU2 (221154)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480119
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 221154 | MICU2 | mitochondrial calcium uptake 2 | NM_152726.3 | 99.9% | 99.5% | 1290delA |
| 2 | human | 221154 | MICU2 | mitochondrial calcium uptake 2 | XR_429214.4 | 62.5% | (many diffs) | |
| 3 | human | 221154 | MICU2 | mitochondrial calcium uptake 2 | XM_017020433.1 | 57.9% | 57.6% | 0_1ins546;744delA |
| 4 | human | 221154 | MICU2 | mitochondrial calcium uptake 2 | XR_001749501.2 | 53.2% | (many diffs) | |
| 5 | mouse | 68514 | Micu2 | mitochondrial calcium uptake 2 | NM_028643.3 | 83.3% | 84.9% | (many diffs) |
| 6 | mouse | 68514 | Micu2 | mitochondrial calcium uptake 2 | XM_006519484.3 | 83.3% | 84.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1368
- ORF length:
- 1302
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcggctgcg ggtagctgcg cgcgggtggc ggcctggggc ggaaaactgc 121 gacgggggct cgctgtcagc cgacaggctg tgcggagtcc cggccccttg gcagcggcag 181 tggccggcgc ggccctggca ggagcaggag cggcctggca ccacagccgc gtcagtgttg 241 cggcgcggga tggcagtttt acagtctccg cacagaaaaa tgttgaacat ggaataatat 301 atattgggaa accgtctctt cgtaagcagc gcttcatgca gttttcttca ctcgaacatg 361 aaggagaata ttatatgaca ccacgagact tcctcttctc agtgatgttt gagcaaatgg 421 aacgtaaaac ttcagtcaag aagctgacaa aaaaggacat cgaggataca ctgtcaggga 481 tccaaacagc tggctgtgga tcaacttttt tcagagacct tggcgataaa gggctaattt 541 catataccga gtatcttttc ttgcttacaa tcctcactaa accccattct ggatttcatg 601 ttgcttttaa aatgctggat acagatggta atgagatgat tgaaaaaagg gaatttttta 661 agctgcagaa gatcataagt aaacaagatg acttgatgac agtgaaaact aatgaaactg 721 gatatcagga agcaatagtg aaagaacctg aaattaacac aactcttcag atgcgtttct 781 ttggaaaaag aggacaaaga aaacttcatt ataaagaatt tcgaagattt atggaaaatt 841 tacaaacaga gattcaagaa atggaattcc ttcagttttc taaaggtttg agtttcatga 901 gaaaagaaga ctttgcagag tggctacttt ttttcactaa cactgaaaat aaagatattt 961 attGGAAAAA TGTGAGAGAG AAGTTGTCAG CAGGAGAGAG CATTAGTTTG GATGAATTCA 1021 AGTCATTTTG CCATTTTACA ACCCACTTGG AAGACTTTGC TATTGCCATG CAGATGTTCA 1081 GTTTAGCTCA TCGTCCTGTC AGACTAGCGG AGTTTAAGAG AGCTGTGAAA GTAGCAACAG 1141 GACAAGAACT CTCAAACAAT ATTTTGGACA CTGTCTTTAA GATCTTTGAT TTGGATGGTG 1201 ATGAATGTCT TAGTCATGAA GAGTTTCTTG GGGTGTTAAA AAACAGAATG CATCGAGGTT 1261 TATGGGTACC ACAACATCAG AGTATACAAG AATACTGGAA GTGTGTGAAG AAAGAAAGCA 1321 TTAAAGGAGT AAAAGAAGTC TGGAAACAAG CTGGAAAGGT CTTTTTTACC CAACTTTCTT 1381 GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC 1441 GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG 1501 TGGAAAGGAC GAGGTCCACT GTTAAACGCC GCTGGGACGC GTTAAGTCga caatcaacct 1561 ctggattaca aaatttgtga aagatt