Construct: ORF TRCN0000480124
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012275.1_s317c1
- Derived from:
- ccsbBroadEn_09848
- DNA Barcode:
- GCGCCAATATGCCTCCGTCAACTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OR10G8 (219869)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480124
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 219869 | OR10G8 | olfactory receptor family 1... | NM_001004464.1 | 99.8% | 100% | 672C>A |
| 2 | human | 219870 | OR10G9 | olfactory receptor family 1... | NM_001001953.1 | 92.6% | 88.1% | (many diffs) |
| 3 | human | 390264 | OR10G4 | olfactory receptor family 1... | NM_001004462.1 | 92.2% | 87.1% | (many diffs) |
| 4 | human | 390265 | OR10G7 | olfactory receptor family 1... | NM_001004463.1 | 92.1% | 88.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1002
- ORF length:
- 933
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtccaacgcc agcctactga cagcgttcat cctcatgggc cttccccatg 121 ccccagcgct ggacgccccc ctctttggag tcttcctggt ggtttacgtg ctcactgtgc 181 tggggaacct cctcatcctg ctggtgatca gggtggattc tcacctccac accaccatgt 241 actacttcct caccaacctg tcgttcattg acatgtggtt ctccactgtc acggtgccca 301 aattgctgat gactttggtg ttcccaagtg gcagggctat ctccttccac agctgcatgg 361 ctcagctcta tttctttcac ttcctagggg gcaccgagtg tttcctctac agggtcatgt 421 cctgtgatcg ctacctggcc atcagttacc cgctcaggta caccagcatg atgactgggc 481 gctcgtgtac tcttctggcc accagcactt ggctcagtgg ctctctgcac tctgctgtcc 541 aggccatatt gactttccat ttgccctact gtggacccaa ctggatccag cactatttgt 601 gtgatgcacc gcccatcctg aaactggcct gtgcagacac cTCAGCCATA GAGACTGTCA 661 TTTTTGTGAC TGTTGGAATA GTGGCCTCGG GCTGCTTTGT CCTGATAGTG CTGTCCTATG 721 TGTCCATCGT CTGTTCCATA CTGCGGATCC GCACCTCAGA GGGGAAGCAC AGAGCCTTTC 781 AGACCTGTGC CTCCCACTGT ATCGTGGTCC TTTGCTTCTT TGGCCCTGGT CTTTTCATTT 841 ACCTGAGGCC AGGCTCCAGG AAAGCTGTGG ATGGAGTTGT GGCCGTTTTC TACACTGTGC 901 TGACGCCCCT TCTCAACCCT GTTGTGTACA CCCTGAGGAA CAAGGAGGTG AAGAAAGCTC 961 TGTTGAAGCT GAAAGACAAA GTAGCACATT CTCAGAGCAA ATTGCCAACT TTCTTGTACA 1021 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1081 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1141 AGGACGAGCG CCAATATGCC TCCGTCAACT CACGCGTTAA GTCgacaatc aacctctgga 1201 ttacaaaatt tgtgaaagat t