Construct: ORF TRCN0000480127
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010878.1_s317c1
- Derived from:
- ccsbBroadEn_14235
- DNA Barcode:
- AGGCGTCCGTGCTTCAGTTAAATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CACHD1 (57685)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480127
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57685 | CACHD1 | cache domain containing 1 | NM_001293274.1 | 99.8% | 99.7% | 353T>C;597C>A;1245C>A |
| 2 | human | 57685 | CACHD1 | cache domain containing 1 | XM_011541863.1 | 85.7% | 85.6% | (many diffs) |
| 3 | human | 57685 | CACHD1 | cache domain containing 1 | XM_011541862.1 | 84.2% | 84.1% | (many diffs) |
| 4 | human | 57685 | CACHD1 | cache domain containing 1 | NM_020925.4 | 76.6% | 76.6% | (many diffs) |
| 5 | mouse | 320508 | Cachd1 | cache domain containing 1 | NM_198037.1 | 68.2% | 73.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3003
- ORF length:
- 2934
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtccaccttt gttagcagcg tgaagtcttc agacagtcct acccagcacg 121 cagtgggatt ccaaaaggca tttcagctga ttcgaagtac aaacaataac acaaagttcc 181 aagcaaatac agacatggtc atcatttacc tgtcagctgg cattacatca aaggactctt 241 cggaagaaga taaaaaagcg actctccaag tcatcaatga agaaaatagc tttctaaaca 301 actctgtaat gattctcacc tatgccctca tgaacgatgg ggtgactggt ttgaaagagc 361 tggcttttct gagggatcta gctgaacaga attcagggaa gtacggtgtg ccagaccgga 421 cggccttgcc tgtgattaag ggcagcatga tggtgctgaa tcagttgagc aacctggaga 481 ccacagtggg caggttctac acaaaccttc ccaaccggat gattgatgaa gccgtcttca 541 gcctgccctt ctctgatgag atgggagatg gtttgataat gactgtgagt aaaccctgtt 601 attttggaaa cctacttctg ggaattgtag gtgtggacgt gaatctggct tacattcttg 661 aagaagtgac gtattaccaa gactctttgg cttcctatac ttttctcata gacgacaaag 721 gatatacact tatgcaccca tctcttacca ggccatattt attgtcagag cccccacttc 781 atactgacat catacattat gaaaatattc caaaatttga attagttcgg caaaatatcc 841 taagcctccc tctgggcagc cagattatcg cagtccctgt gaactcatcc ctgtcttggc 901 acataaacaa gctgagagaa actggaaagg aagcctacaa tgttagctat gcctggaaga 961 tggtacaaga cacttccttt attctgtgta ttgtggtgat acaaccagaa atacctgtga 1021 aacaactgaa gaacctcaac actgttccca gcagcaagct gctgtaccac cggctggatc 1081 tccttggcca gcccagtgct tgcctccact tcaaacagct ggcaacccta gaaagtccca 1141 ccatcatgct gtctgctggc agcttttcct ccccctatga gcacctcagc cagccagaga 1201 caaagcgcat ggtagagcac tacaccgcct atctcagcga caacacccgc ctcattgcta 1261 acccgggcct caaattctct gtcagaaatg aagtaatggc taccagccac gtaacagatg 1321 aatggatgac acaaatggaa atgagtagcc tgaacactta cattgtccgc cgttacatag 1381 caacacccaa tggcgtcctc agaatttatc ctggttccct catggacaaa gcatttgatc 1441 ccactaggag acaatggtat ctccatgcag tagctaatcc agggttgatt tctttgactg 1501 gtccttactt agatgttgga ggagctggtt atgttgtgac aatcagtcac acaattcatt 1561 catccagtac acagctgtct tctgggcaca ctgtggctgt gatgggcatt gacttcacac 1621 tcagatactt ctacaaagtt ctgatggacc tattacctgt ctgtaaccaa gatggtggca 1681 acaaaataag gtgcttcata atggaggaca ggggttatct ggtggcgcac ccgactctca 1741 tcgaccccaa aggacatgca cctgtggagc agcagcacat cacccacaag gagcccctgg 1801 tagcaaatga tatcctcaac caccccaact ttgtaaagaa aaacctgtgc aacagcttca 1861 gtgacagaac ggtccagagg ttttataaat tcaacaccag ccttgcgggg gatttgacga 1921 accttgtgca tggcagccac tgttccaaat acagattagc aaggatccca ggaaccaacg 1981 cgtttgttgg cattgtcaac gaaacctgcg actctcttgc cttctgtgcc tgcagcatgg 2041 tggaccgact ctgtctcaac tgtcaccgaa tggaacaaaa tgaatgtgaa tgtccttgtg 2101 agtgccctct agaggtcaat gagtgcactg gcaacctcac caatgcagag aaccgaaacc 2161 ccagctgcga ggtccaccag gagccggtga catacacagc tattgaccct ggcctgcaag 2221 atgctcttca ccagtgtgtc aacagcaggt gcagtcagag gctggaaagt ggggactgtt 2281 ttggggtgct ggattgtgaa tggtgcatgg tggacagtga tggaaagact cacctggaca 2341 aaccctactg tgccccccag aaagaatgct tcggggggat tgtgggagcc aaaagtccct 2401 acgttgatga catgggagca ataggtgatg aggtgatcac attaaacatg attaaaagcg 2461 cccctgtggg tcctgtggct ggagggatca tgggatgcat catggtcttg gtcctggcgg 2521 tgtatgccta ccgccaccag attcatcgcc ggagccatca gcatatgtct cctcttgctg 2581 cccaagaaat gtcagtgcgt atgtccaacc tggagaatga cagagatgaa agggacgacg 2641 acagccacga agacagaggc atcaTCAGCA ACACTCGGTT TATAGCTGCG GTCATCGAAC 2701 GACATGCACA CAGTCCAGAA AGAAGGCGCC GCTACTGGGG TCGATCAGGA ACAGAAAGTG 2761 ATCATGGTTA CAGCACCATG AGCCCACAGG AGGACAGTGA AAATCCTCCA TGCAACAATG 2821 ACCCCTTGTC AGCCGGGGTC GATGTGGGAA ACCATGATGA GGACTTAGAC CTGGATACCC 2881 CCCCTCAGAC TGCTGCCCTA CTAAGTCACA AGTTCCACCA CTACCGGTCA CACCACCCTA 2941 CACTTCATCA TAGCCACCAC TTACAGGCGG CCGTCACGGT ACACACTGTC GATGCAGAAT 3001 GCTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 3061 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 3121 GGCTTTATAT ATCTTGTGGA AAGGACGAAG GCGTCCGTGC TTCAGTTAAA TCACGCGTTA 3181 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt