Construct: ORF TRCN0000480128

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010269.1_s317c1
Derived from:
ccsbBroadEn_08299
DNA Barcode:
CTCCCACGCCAATAATTAACGCAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NELFCD (51497)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480128
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51497 NELFCD negative elongation factor ... NM_198976.4 98.4% 98.4% 0_1ins27;657C>T
2 human 51497 NELFCD negative elongation factor ... XR_244142.3 74.3% (many diffs)
3 mouse 57314 Nelfcd negative elongation factor ... NM_020580.3 87.3% 97.2% (many diffs)
4 mouse 57314 Nelfcd negative elongation factor ... XM_006499946.2 79.4% 89.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1836
ORF length:
1770
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc gggggccgtg ccgggcgcca tcatggacga ggactactac gggagcgcgg
121ccgagtgggg cgacgaggct gacggcggcc agcaggagga tgattctgga gaaggagagg
181atgatgcgga ggttcagcaa gaatgcctgc ataaattttc cacccgggat tatatcatgg
241aaccctccat cttcaacact ctgaagaggt attttcaggc aggagggtct ccagagaatg
301ttatccagct cttatctgaa aactacaccg ctgtggccca gactgtgaac ctgctggccg
361agtggctcat tcagacaggt gttgagccag tgcaggttca ggaaactgtg gaaaatcact
421tgaagagttt gctgatcaaa cattttgacc cccgcaaagc agattctatt tttactgaag
481aaggagagac cccagcgtgg ctggaacaga tgattgcaca taccacgtgg cgggaccttt
541tttataaact ggctgaagcc catccagact gtttgatgct gaacttcacc gttaagctta
601tttctgacgc agggtaccag ggggagatca ccagtgtgtc cacagcatgc cagcagctag
661aagtgttctc gagagtgctc cggacctctc tagctacaat tttagatgga ggagaagaaa
721accttgaaaa aaatctccct gagtttgcta agatggtgtg ccacggggag cacacgtacc
781tgtttgccca ggccatgatg tccgtgctgg cccaggagga gcaggggggc tccgctgtgc
841gcaggatcgc ccaggaagtg cagcgctttg cccaggagaa aggtcatgac gccagtcaga
901tcacactagc cttgggcaca gctgcctcct accccagggc ctgccaggct ctcggggcca
961tgctgtccaa aggagccctg aaccctgctg acatcaccgt cctgttcaag atgttcacaa
1021gcatggaccc tcctccggtt gaacttatcc gcgttccagc cttcctggac ctgttcatgc
1081agtcactctt taaaccaggg gctcggatca accaggacca caagcacaaa tacatccaca
1141tcttggcgta cgcagcaagc gtggttgaga cctggaagaa gaacaagcga gtgagcatca
1201ataaagatga gctgaagtca acgtcaaaag ctgtcgaaac cgttcacaat ttgtgttgca
1261acgagaacaa aggggcctct gaactagtgg cagaattgag cacactttat cagtgtatta
1321ggtttccagt ggtagcaatg ggtgtgctga agtgggtgga ttggactgta tcagaaccaa
1381ggtactttca gctgcagact gaccataccc ctgtccacct ggcgttgctg gatgagatca
1441gcacctgcca ccagctcctg cacccccagg TCCTGCAGCT GCTTGTTAAG CTTTTTGAGA
1501CTGAGCACTC CCAGCTGGAC GTGATGGAGC AGCTTGAGTT GAAGAAGACA CTGCTGGACA
1561GGATGGTTCA CCTGCTGAGT CGAGGTTATG TACTTCCTGT TGTCAGTTAC ATCCGAAAGT
1621GTCTGGAGAA GCTGGACACT GACATTTCAC TCATTCGCTA TTTTGTCACT GAGGTGCTGG
1681ACGTCATTGC TCCTCCTTAT ACCTCTGACT TCGTGCAACT TTTCCTCCCC ATCCTGGAGA
1741ATGACAGCAT CGCAGGTACC ATCAAAACGG AAGGCGAGCA TGACCCTGTG ACGGAGTTTA
1801TAGCTCACTG CAAATCTAAC TTCATCATGG TGAACTACCC AACTTTCTTG TACAAAGTGG
1861TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
1921CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
1981ACTCCCACGC CAATAATTAA CGCAGACGCG TTAAGTCgac aatcaacctc tggattacaa
2041aatttgtgaa agatt

Download FASTA (ORF) (Full)

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