Construct: ORF TRCN0000480148
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010586.3_s317c1
- Derived from:
- ccsbBroadEn_14659
- DNA Barcode:
- CTGACCTTTGCCAAACTAGGCACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MKNK2 (2872)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480148
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2872 | MKNK2 | MAPK interacting serine/thr... | NM_017572.4 | 38.1% | 38.1% | 1_768del |
| 2 | human | 2872 | MKNK2 | MAPK interacting serine/thr... | XM_024451471.1 | 34.4% | 34.4% | 1_903del |
| 3 | human | 2872 | MKNK2 | MAPK interacting serine/thr... | NM_199054.2 | 32.2% | 27.5% | (many diffs) |
| 4 | mouse | 17347 | Mknk2 | MAP kinase-interacting seri... | XM_017313824.1 | 33.1% | 30% | (many diffs) |
| 5 | mouse | 17347 | Mknk2 | MAP kinase-interacting seri... | NM_021462.4 | 29.8% | 27% | (many diffs) |
| 6 | mouse | 17347 | Mknk2 | MAP kinase-interacting seri... | XM_006513316.1 | 29.8% | 27% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 540
- ORF length:
- 474
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cccggaggta gtggaggcct tcagcgagga ggctagcatc tacgacaagc 121 gctgcgacct gtggagcctg ggcgtcatct tgtatatcct actcagcggc tacccgcccT 181 TCGTGGGCCG CTGTGGCAGC GACTGCGGCT GGGACCGCGG CGAGGCCTGC CCTGCCTGCC 241 AGAACATGCT GTTTGAGAGC ATCCAGGAGG GCAAGTACGA GTTCCCCGAC AAGGACTGGG 301 CCCACATCTC CTGCGCTGCC AAAGACCTCA TCTCCAAGCT GCTGGTCCGT GACGCCAAGC 361 AGAGGCTGAG TGCCGCCCAA GTCCTGCAGC ACCCCTGGGT TCAGGGGTGC GCCCCGGAGA 421 ACACCTTGCC CACTCCCATG GTCCTGCAGA GGTGGGACAG TCACTTCCTC CTCCCTCCCC 481 ACCCCTGTCG CATCCACGTG CGACCTGGAG GACTGGTCAG AACCGTTACT GTGAATGAGT 541 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 601 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 661 TTTATATATC TTGTGGAAAG GACGACTGAC CTTTGCCAAA CTAGGCACAA CGCGTTAAGT 721 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt