Construct: ORF TRCN0000480148

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010586.3_s317c1
Derived from:
ccsbBroadEn_14659
DNA Barcode:
CTGACCTTTGCCAAACTAGGCACA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MKNK2 (2872)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480148
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2872 MKNK2 MAPK interacting serine/thr... NM_017572.4 38.1% 38.1% 1_768del
2 human 2872 MKNK2 MAPK interacting serine/thr... XM_024451471.1 34.4% 34.4% 1_903del
3 human 2872 MKNK2 MAPK interacting serine/thr... NM_199054.2 32.2% 27.5% (many diffs)
4 mouse 17347 Mknk2 MAP kinase-interacting seri... XM_017313824.1 33.1% 30% (many diffs)
5 mouse 17347 Mknk2 MAP kinase-interacting seri... NM_021462.4 29.8% 27% (many diffs)
6 mouse 17347 Mknk2 MAP kinase-interacting seri... XM_006513316.1 29.8% 27% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
540
ORF length:
474
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc cccggaggta gtggaggcct tcagcgagga ggctagcatc tacgacaagc
121gctgcgacct gtggagcctg ggcgtcatct tgtatatcct actcagcggc tacccgcccT
181TCGTGGGCCG CTGTGGCAGC GACTGCGGCT GGGACCGCGG CGAGGCCTGC CCTGCCTGCC
241AGAACATGCT GTTTGAGAGC ATCCAGGAGG GCAAGTACGA GTTCCCCGAC AAGGACTGGG
301CCCACATCTC CTGCGCTGCC AAAGACCTCA TCTCCAAGCT GCTGGTCCGT GACGCCAAGC
361AGAGGCTGAG TGCCGCCCAA GTCCTGCAGC ACCCCTGGGT TCAGGGGTGC GCCCCGGAGA
421ACACCTTGCC CACTCCCATG GTCCTGCAGA GGTGGGACAG TCACTTCCTC CTCCCTCCCC
481ACCCCTGTCG CATCCACGTG CGACCTGGAG GACTGGTCAG AACCGTTACT GTGAATGAGT
541GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
601GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
661TTTATATATC TTGTGGAAAG GACGACTGAC CTTTGCCAAA CTAGGCACAA CGCGTTAAGT
721Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)