Transcript: Mouse XM_006513316.1

PREDICTED: Mus musculus MAP kinase-interacting serine/threonine kinase 2 (Mknk2), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Mknk2 (17347)
Length:
3396
CDS:
233..1612

Additional Resources:

NCBI RefSeq record:
XM_006513316.1
NBCI Gene record:
Mknk2 (17347)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006513316.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000221178 CCTGGACTTCCTGCATAACAA pLKO.1 808 CDS 100% 5.625 3.938 N Mknk2 n/a
2 TRCN0000280553 CCTGGACTTCCTGCATAACAA pLKO_005 808 CDS 100% 5.625 3.938 N Mknk2 n/a
3 TRCN0000221176 GACCGTTTCTACCTGGTGTTT pLKO.1 689 CDS 100% 4.950 3.465 N Mknk2 n/a
4 TRCN0000297864 GACCGTTTCTACCTGGTGTTT pLKO_005 689 CDS 100% 4.950 3.465 N Mknk2 n/a
5 TRCN0000145914 GCAGACCTGTGTCAATCTCAT pLKO.1 526 CDS 100% 4.950 3.465 N Mknk2 n/a
6 TRCN0000006100 GCCTGCCAGAACATGCTGTTT pLKO.1 1169 CDS 100% 4.950 3.465 N MKNK2 n/a
7 TRCN0000221179 CACATCTCCTTTGCTGCCAAA pLKO.1 1238 CDS 100% 4.050 2.835 N Mknk2 n/a
8 TRCN0000280552 CACATCTCCTTTGCTGCCAAA pLKO_005 1238 CDS 100% 4.050 2.835 N Mknk2 n/a
9 TRCN0000221177 CCATCCTAAGCCACATCCATA pLKO.1 729 CDS 100% 4.950 2.970 N Mknk2 n/a
10 TRCN0000280551 CCATCCTAAGCCACATCCATA pLKO_005 729 CDS 100% 4.950 2.970 N Mknk2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006513316.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489397 ATTAACAAAAGCATAAAGCTATTC pLX_317 26.6% 87.4% 92.6% V5 (not translated due to prior stop codon) (many diffs) n/a
2 TRCN0000491691 CTCGCGTGTAATCTAAGGTGCTGG pLX_317 26.4% 87.4% 92.4% V5 (many diffs) n/a
3 TRCN0000488777 TGAGCGTAAACATGGCAATGCACA pLX_317 22.9% 78.1% 78.2% V5 (not translated due to prior stop codon) (many diffs) n/a
4 ccsbBroadEn_10857 pDONR223 100% 29.8% 27% None (many diffs) n/a
5 ccsbBroad304_10857 pLX_304 0% 29.8% 27% V5 (many diffs) n/a
6 TRCN0000472856 AGCCATGCCCCCAAACAGGTTCAT pLX_317 78.5% 29.8% 27% V5 (many diffs) n/a
7 ccsbBroadEn_14659 pDONR223 0% 29.8% 27% None (many diffs) n/a
8 ccsbBroad304_14659 pLX_304 0% 29.8% 27% V5 (many diffs) n/a
9 TRCN0000480148 CTGACCTTTGCCAAACTAGGCACA pLX_317 75.1% 29.8% 27% V5 (many diffs) n/a
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