Construct: ORF TRCN0000480151
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009410.1_s317c1
- Derived from:
- ccsbBroadEn_06042
- DNA Barcode:
- ATTACTATCTTGTGATTCGACGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CRYAB (1410)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480151
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1410 | CRYAB | crystallin alpha B | NM_001289807.1 | 99.8% | 100% | 213G>A |
| 2 | human | 1410 | CRYAB | crystallin alpha B | NM_001289808.2 | 99.8% | 100% | 213G>A |
| 3 | human | 1410 | CRYAB | crystallin alpha B | NM_001368245.1 | 99.8% | 100% | 213G>A |
| 4 | human | 1410 | CRYAB | crystallin alpha B | NM_001885.3 | 99.8% | 100% | 213G>A |
| 5 | human | 1410 | CRYAB | crystallin alpha B | NM_001330379.1 | 61.5% | 61.7% | 0_1ins201;12G>A |
| 6 | human | 1410 | CRYAB | crystallin alpha B | NM_001368246.1 | 61.5% | 61.7% | 0_1ins201;12G>A |
| 7 | mouse | 12955 | Cryab | crystallin, alpha B | NM_001289782.1 | 91% | 97.7% | (many diffs) |
| 8 | mouse | 12955 | Cryab | crystallin, alpha B | NM_001289784.1 | 91% | 97.7% | (many diffs) |
| 9 | mouse | 12955 | Cryab | crystallin, alpha B | NM_001289785.1 | 91% | 97.7% | (many diffs) |
| 10 | mouse | 12955 | Cryab | crystallin, alpha B | NM_009964.3 | 91% | 97.7% | (many diffs) |
| 11 | mouse | 12955 | Cryab | crystallin, alpha B | XM_006509970.3 | 91% | 97.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 591
- ORF length:
- 525
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga catcgccatc caccacccct ggatccgccg ccccttcttt cctttccact 121 cccccagccg cctctttgac cagttcttcg gagagcacct gttggagtct gatcttttcc 181 cgacgtctac ttccctgagt cccttctacc TTCGGCCACC CTCCTTCCTG CGGGCACCCA 241 GCTGGTTTGA CACTGGACTC TCAGAGATGC GCCTGGAAAA GGACAGGTTC TCTGTCAACC 301 TGGATGTGAA GCACTTCTCC CCAGAGGAAC TCAAAGTTAA GGTGTTGGGA GATGTGATTG 361 AGGTGCATGG AAAACATGAA GAGCGCCAGG ATGAACATGG TTTCATCTCC AGGGAGTTCC 421 ACAGGAAATA CCGGATCCCA GCTGATGTAG ACCCTCTCAC CATTACTTCA TCCCTGTCAT 481 CTGATGGGGT CCTCACTGTG AATGGACCAA GGAAACAGGT CTCTGGCCCT GAGCGCACCA 541 TTCCCATCAC CCGTGAAGAG AAGCCTGCTG TCACCGCAGC CCCCAAGAAA TACCCAACTT 601 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 661 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 721 CTTGTGGAAA GGACGAATTA CTATCTTGTG ATTCGACGGA ACGCGTTAAG TCgacaatca 781 acctctggat tacaaaattt gtgaaagatt