Construct: ORF TRCN0000480179
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012281.1_s317c1
- Derived from:
- ccsbBroadEn_01016
- DNA Barcode:
- TTTATCCGATTCACCGTGTAGATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MIPEP (4285)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480179
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4285 | MIPEP | mitochondrial intermediate ... | NM_005932.4 | 99.9% | 99.8% | 1462A>G |
| 2 | human | 4285 | MIPEP | mitochondrial intermediate ... | XM_011535097.2 | 91.1% | 91% | 1_2delATins188;1276A>G |
| 3 | human | 4285 | MIPEP | mitochondrial intermediate ... | XM_011535098.3 | 86.5% | 86.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2205
- ORF length:
- 2139
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gtgcgtcgga aggctgggcg gcttgggagc cagagcagca gctctgccgc 121 cccgccgggc gggccgggga agcctcgaag ccgggatccg ggcccgaagg gtcagcacca 181 gctggtctcc cgtgggcgcc gccttcaatg tcaagcccca gggcagccgc ttggacctgt 241 tcggcgagcg ccggggtctt tttggagttc ctgagctgag tgccccagaa ggatttcata 301 ttgcacaaga aaaagccttg agaaagacag aattgcttgt ggaccgtgca tgttccaccc 361 cacctgggcc ccagaccgtg ctgatcttcg atgagctctc ggattcctta tgcagagtgg 421 ccgacttggc tgattttgtg aaaatcgctc accctgagcc agcattcaga gaagctgcgg 481 aagaagcttg tagaagtatt ggcaccatgg tagagaagtt gaacacaaat gtggatttat 541 atcaaagttt gcaaaaatta ctagctgata aaaaacttgt ggattccctt gatccagaaa 601 caaggcgagt ggctgaactg tttatgtttg attttgaaat tagtggaatc catctagaca 661 aagaaaagcg taaaagagca gtggacctca atgttaaaat cttggatttg agtagtacat 721 ttcttatggg aaccaatttt cccaacaaga ttgagaagca tctcttacca gaacacattc 781 gtcgtaactt tacatctgct ggggatcata tcataattga tggtctccac gcagaatcac 841 cagatgactt ggtgcgagaa gctgcttata aaatttttct ttatcccaat gctggtcaat 901 tgaaatgttt agaagaattg ctcagcagca gagatcttct ggcaaagttg gtggggtatt 961 ccacgttttc tcacagggct ctccaaggaa cgatagctaa aaatccagag actgtcatgc 1021 agttccttga aaaactatct gacaaacttt ctgaaagaac tctgaaagat tttgagatga 1081 tacgagggat gaaaatgaaa ctgaatcctc aaaattccga agtaatgccc tgggaccccc 1141 cttactacag tggtgtgatt cgtgcagaaa ggtataatat tgagcccagc ctatattgcc 1201 cgtttttctc tcttggagca tgcatggaag gcctgaatat tttgcttaac agactgttgg 1261 ggatttcatt atatgcagag cagcctgcaa aaggagaggt gtggagcgaa gatgtccgaa 1321 aactggctgt tgttcatgaa tctgaaggat tgttggggta catttactgt gatttttttc 1381 agcgagcaga caaaccacat caggattgcc atttcactat ccgtggaggc agactaaagg 1441 aagatggaga ctatcaactc ccagttgtag ttcttatgct gaatcttccc cgttcctcaa 1501 ggagttctcc aactttgcta actcctggca tgatggaaaa tcttttccat gaaatgggac 1561 atgccatgca ttcaatgcta ggacgtactc gttaccaaca cgtcactggg accaggtgcc 1621 ctactgattt tgctgaggtt ccttctattc tgatggagta ctttgcaaat gattatcgag 1681 tagttaacca atttgccaga cattatcaga ctggacagcc actgccaaaa aatatggtgt 1741 ctcgtctttg tgaatctaaa aaggtttgtg ctgcagctga tatgcaactt caggtctttt 1801 atgccactct ggatcaaatc taccatggga agcaTCCCCT GAGGAATTCA ACCACAGACA 1861 TTCTCAAGGA AACACAAGAG AAATTCTATG GCCTACCATA TGTTCCAAAT ACTGCCTGGC 1921 AGCTGCGATT CAGCCACCTC GTGGGGTATG GTGCTAGATA TTACTCTTAC CTCATGTCCA 1981 GAGCGGTCGC CTCCATGGTT TGGAAGGAGT GTTTTCTACA GGATCCTTTC AACAGGGCTG 2041 CCGGGGAGCG CTATCGCAGG GAGATGCTGG CCCACGGTGG AGGCAGGGAG CCCATGCTCA 2101 TGGTTGAAGG TATGCTTCAG AAGTGTCCTT CTGTTGATGA CTTCGTAAGT GCCCTCGTTT 2161 CCGACTTGGA TCTGGACTTC GAAACTTTCC TCATGGATTC TGAATACCCA ACTTTCTTGT 2221 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2281 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2341 GAAAGGACGA TTTATCCGAT TCACCGTGTA GATGACGCGT TAAGTCgaca atcaacctct 2401 ggattacaaa atttgtgaaa gatt