Construct: ORF TRCN0000480184
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008870.1_s317c1
- Derived from:
- ccsbBroadEn_13454
- DNA Barcode:
- TCAAACCCCGATCCACCTAGCGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZDHHC20 (253832)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480184
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 253832 | ZDHHC20 | zinc finger DHHC-type conta... | NM_153251.3 | 89.7% | 88.9% | (many diffs) |
| 2 | human | 253832 | ZDHHC20 | zinc finger DHHC-type conta... | XM_006719797.1 | 89.4% | 88.7% | (many diffs) |
| 3 | human | 253832 | ZDHHC20 | zinc finger DHHC-type conta... | XM_006719796.1 | 87.2% | 86.5% | (many diffs) |
| 4 | human | 253832 | ZDHHC20 | zinc finger DHHC-type conta... | NM_001330059.2 | 87% | 86.3% | (many diffs) |
| 5 | human | 253832 | ZDHHC20 | zinc finger DHHC-type conta... | NM_001286638.1 | 71.7% | 70.9% | (many diffs) |
| 6 | human | 253832 | ZDHHC20 | zinc finger DHHC-type conta... | XM_017020527.1 | 66.3% | 65.6% | (many diffs) |
| 7 | human | 253832 | ZDHHC20 | zinc finger DHHC-type conta... | XM_017020525.1 | 65.7% | 63.6% | (many diffs) |
| 8 | human | 253832 | ZDHHC20 | zinc finger DHHC-type conta... | XM_017020526.1 | 64.5% | 63.8% | (many diffs) |
| 9 | human | 253832 | ZDHHC20 | zinc finger DHHC-type conta... | XM_011535030.3 | 40.8% | 34.6% | (many diffs) |
| 10 | human | 253832 | ZDHHC20 | zinc finger DHHC-type conta... | NR_104487.2 | 18% | 1_132del;1093_5322del | |
| 11 | human | 253832 | ZDHHC20 | zinc finger DHHC-type conta... | XR_001749530.1 | 18% | 1_138del;1099_5325del | |
| 12 | human | 253832 | ZDHHC20 | zinc finger DHHC-type conta... | XR_429215.1 | 17.7% | 1_138del;1099_5403del | |
| 13 | human | 253832 | ZDHHC20 | zinc finger DHHC-type conta... | XR_001749531.1 | 17.7% | 1_138del;1099_5406del | |
| 14 | human | 253832 | ZDHHC20 | zinc finger DHHC-type conta... | NR_104486.2 | 17.3% | 1_132del;501_618del;1211_5518del | |
| 15 | human | 253832 | ZDHHC20 | zinc finger DHHC-type conta... | XR_941546.1 | 17.3% | 1_138del;864_1082del;1318_5547del | |
| 16 | mouse | 75965 | Zdhhc20 | zinc finger, DHHC domain co... | NM_029492.4 | 77.7% | 78.2% | (many diffs) |
| 17 | mouse | 75965 | Zdhhc20 | zinc finger, DHHC domain co... | XM_006519661.3 | 63.1% | 63.5% | (many diffs) |
| 18 | mouse | 75965 | Zdhhc20 | zinc finger, DHHC domain co... | XM_006519660.3 | 61.5% | 61.9% | (many diffs) |
| 19 | mouse | 75965 | Zdhhc20 | zinc finger, DHHC domain co... | XM_006519659.3 | 61.4% | 61.8% | (many diffs) |
| 20 | mouse | 75965 | Zdhhc20 | zinc finger, DHHC domain co... | XM_006519657.3 | 60.2% | 60.6% | (many diffs) |
| 21 | mouse | 75965 | Zdhhc20 | zinc finger, DHHC domain co... | XM_006519658.3 | 60.2% | 60.6% | (many diffs) |
| 22 | mouse | 75965 | Zdhhc20 | zinc finger, DHHC domain co... | XR_001781166.1 | 15.3% | (many diffs) | |
| 23 | mouse | 75965 | Zdhhc20 | zinc finger, DHHC domain co... | XR_001781165.1 | 15.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1029
- ORF length:
- 960
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgccctgg acgctgtggc gctgctgcca gcgcgtcgtg ggctgggtgc 121 cggtgctctt catcaccttc gtggtcgtct ggtcctacta cgcgtacgtg gtggagctct 181 gcgtgtttac tatttttgga aatgaagaaa atggaaagac cgttgtttac cttgtggctt 241 tccatctgtt ctttgttatg tttgtatggt cctattggat gacaattttc acatctcccg 301 cttccccctc caaagagttc tacttgtcca attctgaaaa ggaacgttat gaaaaagaat 361 tcagccaaga aagacaacaa gaaattttga gaagagcagc aagagcttta cctatctata 421 ccacatcagc ttcaaaaact atcagatatt gtgaaaaatg tcagctgatt aaacctgatc 481 gggcgcatca ctgctcagcc tgtgactcat gtattcttaa gatggatcat cactgtcctt 541 gggtgaataa ctgtgtggga ttttctaatt acaaattctt cctgctgttt ttattgtatt 601 ccctattata ttgCCTTTTC GTGGCTGCAA CAGTTTTAGA GTACTTTATA AAATTTTGGA 661 CGAATGAACT GACAGATACA CGTGCAAAAT TCCACGTACT TTTTCTTTTC TTTGTGTCTG 721 CAATGTTCTT CATCAGCGTC CTCTCACTTT TCAGCTACCA CTGCTGGCTA GTTGGAAAAA 781 ATAGAACAAC AATAGAATCA TTCCGCGCAC CCACGTTTTC ATACGGACCT GATGGAAATG 841 GTTTCTCTCT TGGATGCAGT AAAAATTGGA GACAAGTCTT TGGTGATGAA AAGAAATATT 901 GGCTACTTCC AATATTTTCA AGCTTGGGTG ATGGTTGCAG TTTTCCAACT CGCCTTGTGG 961 GGATGGATCC AGAACAAGCT TCTGTTACAA ACCAGAATGA GTATGCCAGA AGATCTAAAC 1021 TGCAGAGGTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1081 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1141 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATCAAAC CCCGATCCAC CTAGCGGAAC 1201 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt