Construct: ORF TRCN0000480192
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004620.2_s317c1
- Derived from:
- ccsbBroadEn_08716
- DNA Barcode:
- GCCGACGCGCCTATTTATCTAAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CAMK1G (57172)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480192
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57172 | CAMK1G | calcium/calmodulin dependen... | NM_020439.3 | 99.9% | 100% | 654A>G |
| 2 | human | 57172 | CAMK1G | calcium/calmodulin dependen... | XM_017001866.2 | 99.9% | 100% | 654A>G |
| 3 | human | 57172 | CAMK1G | calcium/calmodulin dependen... | XM_017001867.1 | 66.3% | 66.3% | 0_1ins480;174A>G |
| 4 | mouse | 215303 | Camk1g | calcium/calmodulin-dependen... | NM_144817.2 | 87.7% | 92% | (many diffs) |
| 5 | mouse | 215303 | Camk1g | calcium/calmodulin-dependen... | XM_011238932.2 | 85.2% | 89.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1494
- ORF length:
- 1428
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg tcgaaaggaa gaagatgact gcagttcctg gaagaaacag accaccaaca 121 tccggaaaac cttcattttt atggaagtgc tgggatcagg agctttctca gaagttttcc 181 tggtgaagca aagactgact gggaagctct ttgctctgaa gtgcatcaag aagtcacctg 241 ccttccggga cagcagcctg gagaatgaga ttgctgtgtt gaaaaagatc aagcatgaaa 301 acattgtgac cctggaggac atctatgaga gcaccaccca ctactacctg gtcatgcagc 361 ttgtttctgg tggggagctc tttgaccgga tcctggagcg gggtgtctac acagagaagg 421 atgccagtct ggtgatccag caggtcttgt cggcagtgaa atacctacat gagaatggca 481 tcgtccacag agacttaaag cccgaaaacc tgctttacct tacccctgaa gagaactcta 541 agatcatgat cactgacttt ggtctgtcca agatggaaca gaatggcatc atgtccactg 601 cctgtgggac cccaggctac gtggctccag aagtgctggc ccagaaaccc tacagcaagg 661 ctgtggattg ctggtccatc ggcgtcatca cctacatatt gctctgtgga taccccccgt 721 tctatgaaga aacggagtct aagcttttcg agaagatcaa ggagggctac tatgagtttg 781 agtctccatt ctgggatgac atttctgagt cagccaagga ctttatttgc cacttgcttg 841 agaaggatcc gaacgagcgg tacacctgtg agaaggcctt gagtcatccc tggattgacg 901 gaaacacagc cctccaccgg gacatctacc catcagtcag cctccagatc cagaagaact 961 ttgctaagag caagtggagg caagccttca acgcagcagc tgtggtgcac cacatgagga 1021 agctacacat gaacctgcac agcccgggcg tccgcccaga ggtggagaac aggccgcctg 1081 aaactcaagc cTCAGAAACC TCTAGACCCA GCTCCCCTGA GATCACCATC ACCGAGGCAC 1141 CTGTCCTGGA CCACAGTGTA GCACTCCCTG CCCTGACCCA ATTACCCTGC CAGCATGGCC 1201 GCCGGCCCAC TGCCCCTGGT GGCAGGTCCC TCAACTGCCT GGTCAATGGC TCCCTCCACA 1261 TCAGCAGCAG CCTGGTGCCC ATGCATCAGG GGTCCCTGGC CGCCGGGCCC TGTGGCTGCT 1321 GCTCCAGCTG CCTGAACATT GGGAGCAAAG GAAAGTCCTC CTACTGCTCT GAGCCCACAC 1381 TCCTCAAAAA GGCCAACAAA AAACAGAACT TCAAGTCGGA GGTCATGGTA CCAGTTAAAG 1441 CCAGTGGCAG CTCCCACTGC CGGGCAGGGC AGACTGGAGT CTGTCTCATT ATGTGCCCAA 1501 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1561 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1621 TATCTTGTGG AAAGGACGAG CCGACGCGCC TATTTATCTA AACACGCGTT AAGTCgacaa 1681 tcaacctctg gattacaaaa tttgtgaaag att