Construct: ORF TRCN0000480204
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015899.1_s317c1
- Derived from:
- ccsbBroadEn_12824
- DNA Barcode:
- CCATATTCCCACACGGTTTCGACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPIRE2 (84501)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480204
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84501 | SPIRE2 | spire type actin nucleation... | NM_032451.2 | 81.3% | 81.2% | 1_393del;885_890delGGTCAT;2131C>T |
| 2 | mouse | 234857 | Spire2 | spire homolog 2 (Drosophila) | NM_172287.2 | 67.2% | 68.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1812
- ORF length:
- 1743
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccaacaac gacagcgagg acagcggctg cggtgccgcc gatgagggct 121 acgggggtcc cgaggaggag gaggaggccg agggcgtccc ccgcagcgtg cgcacctttg 181 cccaggccat gcggctgtgc gcggcgcggc tgaccgaccc ccggggcgca caggcgcatt 241 accaggccgt gtgccgcgcg ctcttcgtgg agacgctgga gctgcgggcc ttcctggcca 301 gggtccggga ggccaaggag atgctgcaga agcttcggga ggacgagccg catctggaga 361 cgcctcgggc agagctggac agcctgggtc acacagactg ggcccgactg tgggttcagc 421 tcatgcggga gctccgccgc ggagtgaagc tgaagaaggt gcaagagcag gagttcaacc 481 ccctccccac cgagttccag ctcacgccct tcgagatgct gatgcaggac atccgggccc 541 ggaactacaa gctgcgcaag gtggatgggg acatcccgcc ccgggtgaag aaggacgctc 601 acgagctcat cctggacttt atccgctcac ggcctccact gaagcaggtc tctgagaggc 661 ggctgcgccc gttgccacca aagcaaaggt ccctgcatga gaagatcctg gaggagatca 721 agcaggagcg gaggctgcgc ccggtgcggg gcgagggctg ggctgcccgc gggtttggct 781 ctctgccctg catcctcaac gcctgctccg gagatgccaa gtccacctcc tgcatcaacc 841 tgtcagtcac agatgctggg ggcagcgccc agcgcccgcg gccccgcgtg ctgctcaagg 901 cgcctacctt ggctgaaatg gaagagatga atacatctga ggaagaagag tctccgtgtg 961 gggaggtgac gctgaaacgg gaccgctcct tctcagagca tgacctggcc cagctccgaa 1021 gtgaggtggc ctctggcctg cagtcggcca cccacccccc aggagggacg gagccaccac 1081 ggccccgagc tggcagtgcg catgtgtgga ggcccggctc ccgagaccag ggtacctgtc 1141 ccgcgagtgt ctctgacccc agccaccccc tactcagcaa ccggggctcc tcgggggaca 1201 gacccgaggc ctccatgacc cccgatgcca aacacctgtg gctggagttc agccaccccg 1261 tggagagcct ggcgctgact gtggaagagg tgatggacgt gcgccgtgtg ctggtgaagg 1321 ccgagatgga aaagtttttg cagaacaagg agctcttcag cagtctgaag aagggGAAGA 1381 TTTGCTGCTG CTGCCGGGCC AAGTTCCCGC TGTTCTCGTG GCCGCCCAGC TGTCTCTTCT 1441 GCAAGAGAGC CGTCTGCACT TCCTGTAGCA TAAAGATGAA GATGCCTTCT AAGAAATTTG 1501 GACACATCCC TGTCTACACA CTGGGCTTTG AGAGTCCTCA GAGGGTATCA GCTGCCAAAA 1561 CCGCGCCAAT CCAGAGAAGA GACATCTTTC AGTCTCTGCA AGGGCCACAG TGGCAGAGCG 1621 TGGAGGAGGC GTTCCCCCAC ATCTACTCCC ACGGCTGTGT CCTGAAGGAT GTCTGCAGTG 1681 AGTGCACCAG CTTTGTGGCA GACGTGGTGC GTTCCAGCCG CAAGAGCGTG GACGTCCTCA 1741 ACACTACGCC ACGACGCAGT CGCCAGACCC AATCCCTCTA CATCCCTAAC ACCAGGACTT 1801 TTGACTTCAA GTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1861 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1921 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACCA TATTCCCACA CGGTTTCGAC 1981 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t