Construct: ORF TRCN0000480210
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010811.1_s317c1
- Derived from:
- ccsbBroadEn_07914
- DNA Barcode:
- ATAATCTTTCTGTGCCAGACATGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AMACR (23600)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480210
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23600 | AMACR | alpha-methylacyl-CoA racemase | NM_203382.3 | 99.6% | 99.4% | 25G>A;441T>C |
| 2 | human | 23600 | AMACR | alpha-methylacyl-CoA racemase | NM_014324.6 | 51.6% | 37.5% | (many diffs) |
| 3 | human | 23600 | AMACR | alpha-methylacyl-CoA racemase | NM_001167595.2 | 50% | 36.4% | (many diffs) |
| 4 | human | 100534612 | C1QTNF3-AMACR | C1QTNF3-AMACR readthrough (... | NR_037951.1 | 14.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 660
- ORF length:
- 594
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc actgcagggc atctcggtca tggagctgtc cggcctggcc ccgggcccgt 121 tctgtgctat ggtcctggct gacttcgggg cgcgtgtggt acgcgtggac cggcccggct 181 cccgctacga cgtgagccgc ttgggccggg gcaagcgctc gctagtgctg gacctgaagc 241 agccgcgggg agccgccgtg cTGCGGCGTC TGTGCAAGCG GTCGGATGTG CTGCTGGAGC 301 CCTTCCGCCG CGGTGTCATG GAGAAACTCC AGCTGGGCCC AGAGATTCTG CAGCGGGAAA 361 ATCCAAGGCT TATTTATGCC AGGCTGAGTG GATTTGGCCA GTCAGGAAGC TTCTGCCGGT 421 TAGCTGGCCA CGATATCAAC TATTTGGCTT TGTCAGGTGG AAGGAACAGC ATATTTAAGT 481 TCTTTTCTGT GGAAAACTCA GAAATCGAGT CTGTGGGAAG CACCTCGAGG ACAGAACATG 541 TTGGATGGTG GAGCACCTTT CTATACGACT TACAGGACAG CAGATGGGGA ATTCATGGCT 601 GTTGGAGCAA TAGAACCCCA GTTCTACGAG CTGCTGATCA AAGGACTTGG ACTAAAGTCT 661 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 721 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 781 TTTATATATC TTGTGGAAAG GACGAATAAT CTTTCTGTGC CAGACATGTA CGCGTTAAGT 841 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt