Construct: ORF TRCN0000480235

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016475.1_s317c1
Derived from:
ccsbBroadEn_10427
DNA Barcode:
AGAATCGTTCCCCGGGAAGTCTCT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DHX8 (1659)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480235
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1659 DHX8 DEAH-box helicase 8 NM_004941.3 99.4% 99.4% 1_18del;3102A>G;3202C>A
2 human 1659 DHX8 DEAH-box helicase 8 NM_001322221.1 99.2% 99.2% (many diffs)
3 human 1659 DHX8 DEAH-box helicase 8 NM_001302623.2 94.9% 92.8% (many diffs)
4 human 1659 DHX8 DEAH-box helicase 8 NM_001322219.2 94.8% 92.8% (many diffs)
5 human 1659 DHX8 DEAH-box helicase 8 NM_001322218.2 94.8% 92.7% (many diffs)
6 human 1659 DHX8 DEAH-box helicase 8 NM_001322220.2 93.6% 93.4% (many diffs)
7 human 1659 DHX8 DEAH-box helicase 8 NM_001322217.1 92.9% 92.9% 0_1ins255;2829A>G;2929C>A
8 human 1659 DHX8 DEAH-box helicase 8 NR_136226.1 84.3% (many diffs)
9 human 1659 DHX8 DEAH-box helicase 8 NR_136227.1 80.2% (many diffs)
10 human 1659 DHX8 DEAH-box helicase 8 NR_136225.1 79.8% (many diffs)
11 human 1659 DHX8 DEAH-box helicase 8 NM_001322216.1 77.7% 77.7% 0_1ins807;2277A>G;2377C>A
12 human 1659 DHX8 DEAH-box helicase 8 NR_136228.2 61.6% (many diffs)
13 mouse 217207 Dhx8 DEAH (Asp-Glu-Ala-His) box ... NM_144831.2 87.6% 96.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3708
ORF length:
3642
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc gggagcctta atcgggtcgg agccaggccc cgcggaagaa cttgccaaac
121tcgagtacct gtctttggtg tcaaaggttt gcactgagct ggacaatcac ttggggatca
181acgacaagga ccttgctgaa tttgtgatca gtcttgctga gaaaaatacc acctttgata
241cttttaaggc ttctctcgtc aaaaatggtg cagaatttac ggattctctt attagtaact
301tgctgcgtct catacaaacc atgcggcctc cagcgaagcc ttccactagc aaagatccag
361ttgttaaacc taaaacagaa aaagaaaagc tgaaggaact cttcccagtc ctttgccaac
421cggacaaccc ttctgttcgg accatgttgg atgaagatga tgtgaaagtt gctgtggatg
481tcctgaaaga actggaagct ttaatgccca gcgcagcagg ccaggagaag caaagagatg
541ctgaacaccg ggacaggaca aagaagaaga agcggagtcg aagccgagat cgaaaccgag
601atcgagacag agatagggaa cgaaaccgag atagagacca caagcggaga caccgatccc
661gctctcgatc acgttccagg acccgggaga ggaataaagt gaagtctaga tatcggtcca
721ggagcaggag tcagagtccc cccaaagacc ggaaggaccg ggacaaatat ggagagcgga
781atctggatag atggcgggat aagcatgtgg accgccctcc tccagaagag cccaccattg
841gtgacattta taatggcaaa gttaccagca tcatgcagtt tggttgcttt gtgcagctgg
901aaggactaag gaagcggtgg gaaggcctgg tgcacatctc tgagctccgg cgggagggtc
961gtgtggccaa tgtagctgat gtcgtgagca aaggccagag ggtcaaagtc aaagtgctgt
1021ccttcactgg gaccaagacc agcctgagca tgaaggatgt ggatcaagag actggagaag
1081atctaaaccc aaatagacgg cgaaatcttg tcggggagac caatgaggag acctcaatgc
1141ggaatcctga tagacccact cacttgtccc ttgtcagtgc tcctgaagta gaggacgact
1201cactggaacg caagcgcctc acccgaatct ctgacccaga gaagtgggag atcaaacaga
1261tgattgctgc caatgtcctt tccaaagaag aatttccaga ctttgatgaa gagactggca
1321ttctccctaa ggtggatgat gaagaagatg aggaccttga gattgaattg gttgaggaag
1381agcctccatt cctgagaggg cacactaagc aaagcatgga catgagcccc attaaaattg
1441tcaagaaccc agacggctcc ctctcccaag cagcaatgat gcagagtgcc ttggccaaag
1501aaaggcggga actcaaacag gcccagcggg aagctgagat ggattctatt cccatgggac
1561tcaacaaaca ctgggttgac cctctgcctg atgcggaagg cagacagatt gctgccaaca
1621tgaggggtat tgggatgatg cccaatgata ttcctgagtg gaagaagcat gcctttgggg
1681gcaacaaagc ctcttacgga aaaaagaccc agatgtcaat ccttgagcag agggagagcc
1741tgcccatcta caaactgaag gagcaattgg tccaggccgt ccatgacaat cagatcctga
1801ttgtcattgg tgagacagga tctggaaaga caacacagat cacccagtac ctggcggagg
1861caggctacac ttccaggggc aagattgggt gtacccagcc cagaagagtg gcagctatgt
1921cggtggccaa aagagtgtca gaggagtttg gttgttgctt aggccaagag gtgggctaca
1981ccattcgatt tgaggactgc actagccctg aaacagtcat caagtacatg acagatggga
2041tgttgcttag agagtgcttg attgaccctg acctcactca gtacgcgatc atcatgttgg
2101acgaggcaca tgagaggaca attcacactg atgtgctctt tggattgttg aaaaagacag
2161ttcagaaacg gcaggacatg aagctgattg tcacctcagc caccttggat gcagtgaagt
2221tttctcaata cttctatgaa gctcccattt tcaccatccc aggtcgaaca tatccagtgg
2281aaatactgta cacaaaggaa cctgagacag attatctgga tgccagcctg attactgtta
2341tgcagattca tttaacagaa ccaccaggtg atatcctggt cttcctgact ggtcaggaag
2401aaattgatac tgcttgtgag atcctgtatg aaagaatgaa atccctggga cctgatgttc
2461cagagttaat tatcctccca gtgtactctg ctcttcccag tgagatgcag acccgaatct
2521ttgacccagc tccaccaggc agcagaaagg ttgtgattgc caccaatatc gcagagacat
2581cgctgactat tgatggtatc tactatgtgg tggacccagg attcgtgaaa cagaaagttt
2641acaattccaa gacagggatt gaccagctcg tggtgacgcc tatttctcag gctcaggcaa
2701agcaacgagc tggcagagct gggagaacag gcccagggaa gtgttacagg ttgtacacag
2761aacgtgccta ccgagatgaa atgctgacca ccaacgtgcc ggaaatccag agaaccaact
2821tagcaagcac agtgctgtca ctcaaggcca tgggtatcaa tgatctgctg tcctttgatt
2881tcatggatgc cccacctatg gaaactttga tcacagccat ggagcagctg tacacactgg
2941gggccctgga tgacgagggc ctgctcactc gcttgggccg ccggatggca gagttccctc
3001tggagccaat gctatgcaaa atgctcatca tgtctgtgca tctgggctgc agtgaggaaa
3061tgctgaccat tgtatccatg ctgtctgtgc agaacgtctt ctataggccc aaggataaac
3121aagcccttgc agatcagaag aaggccaagt tccaccagac tgaaggggac cacctcaccc
3181tgctagctgt gtacaactcc tggaagaaca acaagttctc caacccatgg tgctatgaga
3241actttatcaa ggctcgttcc cTGCGCCGGG CCCAGGACAT TCGCAAGCAG ATGTTAGGCA
3301TAATGGACAG ACACAAGCTG GATGTTGTTT CCTGTGGCAA GTCCACAGTC CGAGTGCAGA
3361AGGCCATCTG CAGTGGGTTC TTCCGTAATG CTGCCAAGAA AGACCCGCAG GAGGGTTACC
3421GGACACTGAT CGACCAGCAG GTGGTCTATA TCCATCCTTC CAGTGCCCTC TTCAACAGAC
3481AGCCAGAATG GGTGGTGTAC CATGAGCTGG TGCTCACCAC CAAGGAATAC ATGCGTGAAG
3541TTACCACCAT CGACCCTCGG TGGCTTGTGG AGTTTGCCCC AGCCTTCTTC AAGGTCTCAG
3601ACCCAACTAA GCTAAGCAAA CAGAAGAAGC AACAGCGTCT TGAACCCTTG TACAACCGCT
3661ATGAGGAACC CAATGCCTGG AGAATATCTC GAGCTTTCCG ACGGCGCTGC CCAACTTTCT
3721TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
3781CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
3841GTGGAAAGGA CGAAGAATCG TTCCCCGGGA AGTCTCTACG CGTTAAGTCg acaatcaacc
3901tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)