Construct: ORF TRCN0000480235
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016475.1_s317c1
- Derived from:
- ccsbBroadEn_10427
- DNA Barcode:
- AGAATCGTTCCCCGGGAAGTCTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DHX8 (1659)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480235
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1659 | DHX8 | DEAH-box helicase 8 | NM_004941.3 | 99.4% | 99.4% | 1_18del;3102A>G;3202C>A |
| 2 | human | 1659 | DHX8 | DEAH-box helicase 8 | NM_001322221.1 | 99.2% | 99.2% | (many diffs) |
| 3 | human | 1659 | DHX8 | DEAH-box helicase 8 | NM_001302623.2 | 94.9% | 92.8% | (many diffs) |
| 4 | human | 1659 | DHX8 | DEAH-box helicase 8 | NM_001322219.2 | 94.8% | 92.8% | (many diffs) |
| 5 | human | 1659 | DHX8 | DEAH-box helicase 8 | NM_001322218.2 | 94.8% | 92.7% | (many diffs) |
| 6 | human | 1659 | DHX8 | DEAH-box helicase 8 | NM_001322220.2 | 93.6% | 93.4% | (many diffs) |
| 7 | human | 1659 | DHX8 | DEAH-box helicase 8 | NM_001322217.1 | 92.9% | 92.9% | 0_1ins255;2829A>G;2929C>A |
| 8 | human | 1659 | DHX8 | DEAH-box helicase 8 | NR_136226.1 | 84.3% | (many diffs) | |
| 9 | human | 1659 | DHX8 | DEAH-box helicase 8 | NR_136227.1 | 80.2% | (many diffs) | |
| 10 | human | 1659 | DHX8 | DEAH-box helicase 8 | NR_136225.1 | 79.8% | (many diffs) | |
| 11 | human | 1659 | DHX8 | DEAH-box helicase 8 | NM_001322216.1 | 77.7% | 77.7% | 0_1ins807;2277A>G;2377C>A |
| 12 | human | 1659 | DHX8 | DEAH-box helicase 8 | NR_136228.2 | 61.6% | (many diffs) | |
| 13 | mouse | 217207 | Dhx8 | DEAH (Asp-Glu-Ala-His) box ... | NM_144831.2 | 87.6% | 96.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3708
- ORF length:
- 3642
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gggagcctta atcgggtcgg agccaggccc cgcggaagaa cttgccaaac 121 tcgagtacct gtctttggtg tcaaaggttt gcactgagct ggacaatcac ttggggatca 181 acgacaagga ccttgctgaa tttgtgatca gtcttgctga gaaaaatacc acctttgata 241 cttttaaggc ttctctcgtc aaaaatggtg cagaatttac ggattctctt attagtaact 301 tgctgcgtct catacaaacc atgcggcctc cagcgaagcc ttccactagc aaagatccag 361 ttgttaaacc taaaacagaa aaagaaaagc tgaaggaact cttcccagtc ctttgccaac 421 cggacaaccc ttctgttcgg accatgttgg atgaagatga tgtgaaagtt gctgtggatg 481 tcctgaaaga actggaagct ttaatgccca gcgcagcagg ccaggagaag caaagagatg 541 ctgaacaccg ggacaggaca aagaagaaga agcggagtcg aagccgagat cgaaaccgag 601 atcgagacag agatagggaa cgaaaccgag atagagacca caagcggaga caccgatccc 661 gctctcgatc acgttccagg acccgggaga ggaataaagt gaagtctaga tatcggtcca 721 ggagcaggag tcagagtccc cccaaagacc ggaaggaccg ggacaaatat ggagagcgga 781 atctggatag atggcgggat aagcatgtgg accgccctcc tccagaagag cccaccattg 841 gtgacattta taatggcaaa gttaccagca tcatgcagtt tggttgcttt gtgcagctgg 901 aaggactaag gaagcggtgg gaaggcctgg tgcacatctc tgagctccgg cgggagggtc 961 gtgtggccaa tgtagctgat gtcgtgagca aaggccagag ggtcaaagtc aaagtgctgt 1021 ccttcactgg gaccaagacc agcctgagca tgaaggatgt ggatcaagag actggagaag 1081 atctaaaccc aaatagacgg cgaaatcttg tcggggagac caatgaggag acctcaatgc 1141 ggaatcctga tagacccact cacttgtccc ttgtcagtgc tcctgaagta gaggacgact 1201 cactggaacg caagcgcctc acccgaatct ctgacccaga gaagtgggag atcaaacaga 1261 tgattgctgc caatgtcctt tccaaagaag aatttccaga ctttgatgaa gagactggca 1321 ttctccctaa ggtggatgat gaagaagatg aggaccttga gattgaattg gttgaggaag 1381 agcctccatt cctgagaggg cacactaagc aaagcatgga catgagcccc attaaaattg 1441 tcaagaaccc agacggctcc ctctcccaag cagcaatgat gcagagtgcc ttggccaaag 1501 aaaggcggga actcaaacag gcccagcggg aagctgagat ggattctatt cccatgggac 1561 tcaacaaaca ctgggttgac cctctgcctg atgcggaagg cagacagatt gctgccaaca 1621 tgaggggtat tgggatgatg cccaatgata ttcctgagtg gaagaagcat gcctttgggg 1681 gcaacaaagc ctcttacgga aaaaagaccc agatgtcaat ccttgagcag agggagagcc 1741 tgcccatcta caaactgaag gagcaattgg tccaggccgt ccatgacaat cagatcctga 1801 ttgtcattgg tgagacagga tctggaaaga caacacagat cacccagtac ctggcggagg 1861 caggctacac ttccaggggc aagattgggt gtacccagcc cagaagagtg gcagctatgt 1921 cggtggccaa aagagtgtca gaggagtttg gttgttgctt aggccaagag gtgggctaca 1981 ccattcgatt tgaggactgc actagccctg aaacagtcat caagtacatg acagatggga 2041 tgttgcttag agagtgcttg attgaccctg acctcactca gtacgcgatc atcatgttgg 2101 acgaggcaca tgagaggaca attcacactg atgtgctctt tggattgttg aaaaagacag 2161 ttcagaaacg gcaggacatg aagctgattg tcacctcagc caccttggat gcagtgaagt 2221 tttctcaata cttctatgaa gctcccattt tcaccatccc aggtcgaaca tatccagtgg 2281 aaatactgta cacaaaggaa cctgagacag attatctgga tgccagcctg attactgtta 2341 tgcagattca tttaacagaa ccaccaggtg atatcctggt cttcctgact ggtcaggaag 2401 aaattgatac tgcttgtgag atcctgtatg aaagaatgaa atccctggga cctgatgttc 2461 cagagttaat tatcctccca gtgtactctg ctcttcccag tgagatgcag acccgaatct 2521 ttgacccagc tccaccaggc agcagaaagg ttgtgattgc caccaatatc gcagagacat 2581 cgctgactat tgatggtatc tactatgtgg tggacccagg attcgtgaaa cagaaagttt 2641 acaattccaa gacagggatt gaccagctcg tggtgacgcc tatttctcag gctcaggcaa 2701 agcaacgagc tggcagagct gggagaacag gcccagggaa gtgttacagg ttgtacacag 2761 aacgtgccta ccgagatgaa atgctgacca ccaacgtgcc ggaaatccag agaaccaact 2821 tagcaagcac agtgctgtca ctcaaggcca tgggtatcaa tgatctgctg tcctttgatt 2881 tcatggatgc cccacctatg gaaactttga tcacagccat ggagcagctg tacacactgg 2941 gggccctgga tgacgagggc ctgctcactc gcttgggccg ccggatggca gagttccctc 3001 tggagccaat gctatgcaaa atgctcatca tgtctgtgca tctgggctgc agtgaggaaa 3061 tgctgaccat tgtatccatg ctgtctgtgc agaacgtctt ctataggccc aaggataaac 3121 aagcccttgc agatcagaag aaggccaagt tccaccagac tgaaggggac cacctcaccc 3181 tgctagctgt gtacaactcc tggaagaaca acaagttctc caacccatgg tgctatgaga 3241 actttatcaa ggctcgttcc cTGCGCCGGG CCCAGGACAT TCGCAAGCAG ATGTTAGGCA 3301 TAATGGACAG ACACAAGCTG GATGTTGTTT CCTGTGGCAA GTCCACAGTC CGAGTGCAGA 3361 AGGCCATCTG CAGTGGGTTC TTCCGTAATG CTGCCAAGAA AGACCCGCAG GAGGGTTACC 3421 GGACACTGAT CGACCAGCAG GTGGTCTATA TCCATCCTTC CAGTGCCCTC TTCAACAGAC 3481 AGCCAGAATG GGTGGTGTAC CATGAGCTGG TGCTCACCAC CAAGGAATAC ATGCGTGAAG 3541 TTACCACCAT CGACCCTCGG TGGCTTGTGG AGTTTGCCCC AGCCTTCTTC AAGGTCTCAG 3601 ACCCAACTAA GCTAAGCAAA CAGAAGAAGC AACAGCGTCT TGAACCCTTG TACAACCGCT 3661 ATGAGGAACC CAATGCCTGG AGAATATCTC GAGCTTTCCG ACGGCGCTGC CCAACTTTCT 3721 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 3781 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 3841 GTGGAAAGGA CGAAGAATCG TTCCCCGGGA AGTCTCTACG CGTTAAGTCg acaatcaacc 3901 tctggattac aaaatttgtg aaagatt