Construct: ORF TRCN0000480254
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001925.1_s317c1
- Derived from:
- ccsbBroadEn_09034
- DNA Barcode:
- TTTCTACCAGGTAGTATGAAGTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DCAF11 (80344)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480254
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80344 | DCAF11 | DDB1 and CUL4 associated fa... | NM_001163484.1 | 99.8% | 99.8% | 620G>A;648C>T |
| 2 | human | 80344 | DCAF11 | DDB1 and CUL4 associated fa... | NM_025230.4 | 99.8% | 99.8% | 620G>A;648C>T |
| 3 | human | 80344 | DCAF11 | DDB1 and CUL4 associated fa... | NM_181357.2 | 95.1% | 95% | 129_130ins78;542G>A;570C>T |
| 4 | human | 80344 | DCAF11 | DDB1 and CUL4 associated fa... | NR_028100.1 | 40.6% | (many diffs) | |
| 5 | human | 80344 | DCAF11 | DDB1 and CUL4 associated fa... | NR_028099.1 | 35.9% | (many diffs) | |
| 6 | mouse | 28199 | Dcaf11 | DDB1 and CUL4 associated fa... | NM_001199009.1 | 89.2% | 93.4% | (many diffs) |
| 7 | mouse | 28199 | Dcaf11 | DDB1 and CUL4 associated fa... | NM_133734.3 | 89.2% | 93.4% | (many diffs) |
| 8 | mouse | 28199 | Dcaf11 | DDB1 and CUL4 associated fa... | XM_006519081.3 | 89.2% | 93.4% | (many diffs) |
| 9 | mouse | 28199 | Dcaf11 | DDB1 and CUL4 associated fa... | XM_006519083.3 | 89.2% | 93.4% | (many diffs) |
| 10 | mouse | 28199 | Dcaf11 | DDB1 and CUL4 associated fa... | XM_006519084.3 | 89.2% | 93.4% | (many diffs) |
| 11 | mouse | 28199 | Dcaf11 | DDB1 and CUL4 associated fa... | XM_006519085.3 | 89.2% | 93.4% | (many diffs) |
| 12 | mouse | 28199 | Dcaf11 | DDB1 and CUL4 associated fa... | XM_006519086.3 | 89.2% | 93.4% | (many diffs) |
| 13 | mouse | 28199 | Dcaf11 | DDB1 and CUL4 associated fa... | XM_006519087.3 | 81.8% | 84.8% | (many diffs) |
| 14 | mouse | 28199 | Dcaf11 | DDB1 and CUL4 associated fa... | XM_006519088.3 | 81.8% | 84.8% | (many diffs) |
| 15 | mouse | 28199 | Dcaf11 | DDB1 and CUL4 associated fa... | XM_006519089.2 | 81.8% | 84.8% | (many diffs) |
| 16 | mouse | 28199 | Dcaf11 | DDB1 and CUL4 associated fa... | XM_006519090.3 | 81.8% | 84.8% | (many diffs) |
| 17 | mouse | 28199 | Dcaf11 | DDB1 and CUL4 associated fa... | XM_006519091.3 | 81.8% | 84.8% | (many diffs) |
| 18 | mouse | 28199 | Dcaf11 | DDB1 and CUL4 associated fa... | NR_037572.1 | 54.6% | (many diffs) | |
| 19 | mouse | 28199 | Dcaf11 | DDB1 and CUL4 associated fa... | XM_006519092.3 | 49.2% | 50% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1707
- ORF length:
- 1638
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggatcgcgg aacagcagca gtgcaggatc cgggtccgga gacccctccg 121 agggcttgcc ccgaagaggg gctggcctgc gtcggagtga ggaagaggaa gaagaggatg 181 aagatgtgga tctggcccag gtactggcct atctcctccg cagaggccaa gtgaggttgg 241 tgcagggagg aggtgcagca aatttacaat tcattcaggc cctcttggac tcagaggaag 301 agaatgacag agcttgggat ggtcgtcttg gggatcgata caacccacct gtggatgcta 361 cccctgacac ccgggagctg gaattcaatg agatcaagac acaagtggaa ctggccacag 421 ggcagctggg gcttaggcgg gccgcccaga agcacagctt tcctcgaatg ttgcaccaga 481 gagaacgggg cctctgccat cggggaagct tctcccttgg agaacagtct cgagtgatat 541 ctcacttctt gcccaatgat ctgggcttca ctgatagcta ctctcagaag gctttctgtg 601 gcatctacag caaagatggt caaatattca tgtctgcttg ccaagaccag acaatccgac 661 tctatgactg ccgatatggc cgtttccata aattcaagag catcaaggcc cgcgatgtag 721 gctggagcgt cttggatgtg gccttcaccc ctgatgggaa ccacttcctc tactctagct 781 ggtctgatta cattcatatc tgcaatatct atggtgaggg agatacacac actgccctgg 841 atctcaggcc agatgagcgt cgctttgctg tcttctccat tgctgtctcc tcagatggac 901 gagaagtact aggaggggcc aatgatggct gcctgtatgt ctttgaccga gaacagaacc 961 ggcgcaccct tcagattgag tcccatgagg atgatgtgaa tgcagtggcc tttgctgata 1021 taagctccca aatcctgttc tctgggggag atgatgccat ctgcaaagtg tgggatcgac 1081 gcaccatgcg ggaggatgac cccaagcctg tgggtgcact ggctggacac caggatggca 1141 tcaccttcat tgacagcaag ggtgatgccc ggtatctgat ctccaactct aaagaccaga 1201 ccatcaaact ctgggatatc cgacgctttt ccagccggga aggcatggaa gcttcacgcc 1261 aggctgccac acagcaaaac tgggactatc ggtggcagca agtgcccaaa aaagcctggc 1321 ggaagctgaa gctcccaggg gacagctcct tgatgaccta ccggggccac ggagtgctgc 1381 acacccTCAT CCGCTGCCGG TTCTCCCCCA TTCATAGCAC TGGCCAGCAG TTCATCTACA 1441 GTGGCTGCTC CACTGGCAAA GTGGTTGTGT ACGACCTTCT AAGTGGCCAC ATTGTGAAGA 1501 AGCTGACCAA CCACAAGGCC TGTGTGCGTG ACGTCAGTTG GCACCCCTTT GAAGAGAAGA 1561 TTGTCAGCAG TTCGTGGGAC GGGAACCTGC GTCTGTGGCA GTACCGCCAG GCTGAGTACT 1621 TCCAGGATGA CATGCCAGAA TCTGAGGAAT GTGCCAGCGC CCCTGCCCCA GTGCCCCAAT 1681 CCTCTACACC CTTTTCCTCA CCCCAGTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 1741 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1801 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATTTCTACC 1861 AGGTAGTATG AAGTAAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1921 aagatt