Construct: ORF TRCN0000480258
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000041.1_s317c1
- Derived from:
- ccsbBroadEn_14375
- DNA Barcode:
- TAGTTGTTTCACCTAATCAATGGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- FLACC1 (130540)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480258
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 130540 | FLACC1 | flagellum associated contai... | NM_001127391.3 | 99.7% | 61.6% | 127G>C;772_773insAA |
| 2 | human | 130540 | FLACC1 | flagellum associated contai... | NM_001289993.2 | 99.7% | 61.6% | 127G>C;772_773insAA |
| 3 | human | 130540 | FLACC1 | flagellum associated contai... | XM_011510606.3 | 98.5% | 60.8% | 127G>C;772_773insAA;941_955del |
| 4 | human | 130540 | FLACC1 | flagellum associated contai... | NM_139163.3 | 94.6% | 58.4% | 127G>C;772_773insAA;941_1009del |
| 5 | human | 130540 | FLACC1 | flagellum associated contai... | XM_024452696.1 | 94.6% | 58.4% | 127G>C;772_773insAA;941_1009del |
| 6 | human | 130540 | FLACC1 | flagellum associated contai... | XM_024452697.1 | 89.3% | 67.5% | (many diffs) |
| 7 | human | 130540 | FLACC1 | flagellum associated contai... | XM_017003361.2 | 75.6% | 80.4% | (many diffs) |
| 8 | human | 130540 | FLACC1 | flagellum associated contai... | XM_011510610.3 | 71.5% | 35.5% | 0_1ins309;463_464insAA;632_700del |
| 9 | human | 130540 | FLACC1 | flagellum associated contai... | XM_011510612.3 | 47.7% | 11.6% | 0_1ins627;145_146insAA;314_382del |
| 10 | human | 130540 | FLACC1 | flagellum associated contai... | XM_017003362.2 | 47.7% | 11.6% | 0_1ins627;145_146insAA;314_382del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 852
- ORF length:
- 786
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgta ccccaaccct ctcatctact gcacctgctg ggacccctgg aacttgggac 121 cacggaagct aatcaagacc cctcaactac cacgcaagaa ctccacaggg agttccaagc 181 taactcctct tctaccagct ccaaaaaatc acaattacct ccaaccaaca aaacctgttg 241 tttccccaaa aatgaaaatc cattcagcaa ggcaagaaga gactaataaa tcattttatg 301 aagtgatcaa cgtgtcacct ggctatcaac ttgttcggaa tcgggaacag atttctgtca 361 ccttagggga tgagatgttt gataggaaaa agcggtggga atcggagatc ccggacaaag 421 gcagattttc caggaccaac atcatttctg acctagaaga gcaaatctca gagctgacag 481 caataattga acaaatgaac agagaccacc agtctgccca gaaattgctc tccagtgaaa 541 tggatctccg ctgtgctgag atgaaacaga actttgaaaa caagaacagg gagctcaaag 601 aggcccatga agcagaactc agtgagttgg agaacaacta caaagcagcc ttgaaggcag 661 agaagttggc tgcccaagag aagctagagg agatgggaaa agaatacaag tatttgaaga 721 atatgtttcg tacgtatcag gacagtattt atgatgaaat ggaagagaag tggtcaaaac 781 agaaggcgaa atggaagaag gatgagaagt tcgagcgaga aaatatcctg ctacagcaaa 841 aaaaaaaaag atgaccaaaa aattcgaaat ggagtcagga gaagaagata agaaaataaa 901 tgaatcctgc agtgctgtct ttgagaactt cattcaagag aaggaggagc tcttgaaaca 961 acatcaaagt gacaccttgc aattagaaga gctgagaaaa accaaagagg TCATGCAGGA 1021 AGAATTGCAT GCACAAGCCC TTATCCTAGA GTCACTGAAC ACAAACCTCT ACTATACCCA 1081 GTTGGAACTC CAGAAAGAGA AAGCTATAGT GGGAAATCTG GAGAAAATGC TTCAAACCAA 1141 GTTTGCTGAA ACTGAAGAAA AGTATAAGCA CACCATACAG ATCCTGACGG AAGAGAACAT 1201 TCATCTGAAG CAAAAGATAA TTTCTAAGAA TGAAGAAATT TGTGAAGGAT GTTCTGGGAG 1261 ATTGGCCTCT ATTACTGTTT CTAAGGATGA TTCTGACACT GTGCAAGATG GTAGCAAGAA 1321 AGGACAAGAA TCATACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC 1381 TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT 1441 TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT AGTTGTTTCA CCTAATCAAT 1501 GGTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag att