Construct: ORF TRCN0000480271
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008161.1_s317c1
- Derived from:
- ccsbBroadEn_01770
- DNA Barcode:
- TGAGCGGCTGTTCCAGTTAATACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EZR (7430)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480271
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7430 | EZR | ezrin | NM_001111077.2 | 100% | 100% | |
| 2 | human | 7430 | EZR | ezrin | NM_003379.5 | 100% | 100% | |
| 3 | human | 7430 | EZR | ezrin | XM_011536110.1 | 76.7% | 74.2% | 0_1ins37;57_58ins371 |
| 4 | mouse | 22350 | Ezr | ezrin | NM_009510.2 | 88.6% | 96.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1824
- ORF length:
- 1758
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gaaaccaatc aatgtccgag ttaccaccat ggatgcagag ctggagtttg 121 caatccagcc aaatacaact ggaaaacagc tttttgatca ggtggtaaag actatcggcc 181 tccgggaagt gtggtacttt ggcctccact atgtggataa taaaggattt cctacctggc 241 tgaagctgga taagaaggtg tctgcccagg aggtcaggaa ggagaatccc ctccagttca 301 agttccgggc caagttctac cctgaagatg tggctgagga gctcatccag gacatcaccc 361 agaaactttt cttcctccaa gtgaaggaag gaatccttag cgatgagatc tactgccccc 421 ctgagactgc cgtgctcttg gggtcctacg ctgtgcaggc caagtttggg gactacaaca 481 aagaagtgca caagtctggg tacctcagct ctgagcggct gatccctcaa agagtgatgg 541 accagcacaa acttaccagg gaccagtggg aggaccggat ccaggtgtgg catgcggaac 601 accgtgggat gctcaaagat aatgctatgt tggaatacct gaagattgct caggacctgg 661 aaatgtatgg aatcaactat ttcgagataa aaaacaagaa aggaacagac ctttggcttg 721 gagttgatgc ccttggactg aatatttatg agaaagatga taagttaacc ccaaagattg 781 gctttccttg gagtgaaatc aggaacatct ctttcaatga caaaaagttt gtcattaaac 841 ccatcgacaa gaaggcacct gactttgtgt tttatgcccc acgtctgaga atcaacaagc 901 ggatcctgca gctctgcatg ggcaaccatg agttgtatat gcgccgcagg aagcctgaca 961 ccatcgaggt gcagcagatg aaggcccagg cccgggagga gaagcatcag aagcagctgg 1021 agcggcaaca gctggaaaca gagaagaaaa ggagagaaac cgtggagaga gagaaagagc 1081 agatgatgcg cgagaaggag gagttgatgc tgcggctgca ggactatgag gagaagacaa 1141 agaaggcaga gagagagctc tcggagcaga ttcagagggc cctgcagctg gaggaggaga 1201 ggaagcgggc acaggaggag gccgagcgcc tagaggctga ccgtatggct gcactgcggg 1261 ctaaggagga gctggagaga caggcggtgg atcagataaa gagccaggag cagctggctg 1321 cggagcttgc agaatacact gccaagattg ccctccTGGA AGAGGCGCGG AGGCGCAAGG 1381 AGGATGAAGT TGAAGAGTGG CAGCACAGGG CCAAAGAAGC CCAGGATGAC CTGGTGAAGA 1441 CCAAGGAGGA GCTGCACCTG GTGATGACAG CACCCCCGCC CCCACCACCC CCCGTGTACG 1501 AGCCGGTGAG CTACCATGTC CAGGAGAGCT TGCAGGATGA GGGCGCAGAG CCCACGGGCT 1561 ACAGCGCGGA GCTGTCTAGT GAGGGCATCC GGGATGACCG CAATGAGGAG AAGCGCATCA 1621 CTGAGGCAGA GAAGAACGAG CGTGTGCAGC GGCAGCTGCT GACGCTGAGC AGCGAGCTGT 1681 CCCAGGCCCG AGATGAGAAT AAGAGGACCC ACAATGACAT CATCCACAAC GAGAACATGA 1741 GGCAAGGCCG GGACAAGTAC AAGACGCTGC GGCAGATCCG GCAGGGCAAC ACCAAGCAGC 1801 GCATCGACGA GTTCGAGGCC CTGTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1861 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1921 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT GAGCGGCTGT 1981 TCCAGTTAAT ACAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2041 att