Construct: ORF TRCN0000480296
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011399.1_s317c1
- Derived from:
- ccsbBroadEn_12855
- DNA Barcode:
- TGTATGCGTTACCGACTAGGCAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRIM51 (84767)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480296
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 84767 | TRIM51 | tripartite motif-containing 51 | NM_032681.4 | 64.8% | 64.8% | 1_477del |
2 | human | 283116 | TRIM49B | tripartite motif containing... | NM_001206626.1 | 55.7% | 49.1% | (many diffs) |
3 | human | 642612 | TRIM49C | tripartite motif containing... | NM_001195234.1 | 55.2% | 47.7% | (many diffs) |
4 | human | 57093 | TRIM49 | tripartite motif containing 49 | NM_020358.2 | 55.2% | 47.5% | (many diffs) |
5 | human | 399940 | TRIM51EP | tripartite motif-containing... | NR_146921.1 | 50.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 945
- ORF length:
- 879
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga aaccacaaga accagatgct ggaaggatta tgtgagttta aggatagaag 121 caatcagagc tgaatatcag aagatgcctg catttctcca tgaagaagag caacatcact 181 tggaaaggct gcgaaaggag ggcgaggaca tttttcagca actcaatgaa agcaaagcca 241 gaatggaaca ttccagggag cttttaagag gaatgtatga ggatctgaag caaatgtgcc 301 ataaagcaga tgtggagcta ctccaggctt ttggagacat attacacagg tatgagtctc 361 tgctgctgca agtgtctgag cctgtgaatc cagagctcag tgcagggccc atcactggac 421 tgctggacag cctcagtgga ttcagagttg attttactct gcagcctgaa agagccaata 481 gtcataTCTT CCTGTGTGGA GATTTGAGAA GCATGAATGT TGGATGTGAC CCTCAAGATG 541 ATCCCGATAT CACTGGAAAA TCTGAATGTT TTCTTGTATG GGGGGCTCAG GCTTTCACAT 601 CTGGCAAATA TTATTGGGAG GTTCATATGG GGGACTCTTG GAATTGGGCT TTTGGTGTCT 661 GTAACAATTA TTGGAAAGAG AAGAGACAGA ATGACAAGAT AGATGGAGAG GAGGGACTCT 721 TTCTTCTTGG ATGTGTTAAG GAGGACACTC ACTGCAGTCT CTTTACCACC TCCCCACTTG 781 TGGTGCAATA TGTTCCAAGA CCTACCAGCA CAGTAGGATT ATTCCTGGAT TGTGAAGGTA 841 GAACCGTGAG CTTTGTTGAT GTTGATCAAA GTTCCCTGAT ATACACCATC CCCAATTGCT 901 CCTTCTCACC TCCTCTCAGG CCTATCTTTT GCTGTAGTCA CTTCTGCCCA ACTTTCTTGT 961 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1021 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1081 GAAAGGACGA TGTATGCGTT ACCGACTAGG CAAGACGCGT TAAGTCgaca atcaacctct 1141 ggattacaaa atttgtgaaa gatt