Transcript: Human NR_146921.1

Homo sapiens tripartite motif-containing 51E, pseudogene (TRIM51EP), non-coding RNA.

Source:
NCBI, updated 2018-05-12
Taxon:
Homo sapiens (human)
Gene:
TRIM51EP (399940)
Length:
1694
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_146921.1
NBCI Gene record:
TRIM51EP (399940)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_146921.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000237916 CTGAAGCAAATGTACCATAAA pLKO_005 787 3UTR 100% 13.200 7.920 N TRIM51EP n/a
2 TRCN0000236714 GTTCTTGCTCAGTGCTCTAAA pLKO_005 232 3UTR 100% 13.200 7.920 N TRIM51BP n/a
3 TRCN0000237914 TTAGCTCTGAGGAGCAAATAT pLKO_005 347 3UTR 100% 15.000 7.500 Y TRIM51EP n/a
4 TRCN0000034017 CAGTGGATTCAGAGTTGATTT pLKO.1 1009 3UTR 100% 13.200 6.600 Y TRIM51 n/a
5 TRCN0000244288 CCAGATGCTGGAAGGATTATG pLKO_005 584 3UTR 100% 13.200 6.600 Y TRIM51EP n/a
6 TRCN0000427243 CTACCAGCACAGTAGGATTAT pLKO_005 1377 3UTR 100% 13.200 6.600 Y TRIM51 n/a
7 TRCN0000236713 GAAAGGAGGGCGAGGACATTT pLKO_005 695 3UTR 100% 13.200 6.600 Y TRIM51BP n/a
8 TRCN0000244286 ATCAGAAGATGCCTGCATTTC pLKO_005 638 3UTR 100% 10.800 5.400 Y TRIM51BP n/a
9 TRCN0000034015 CCCTCAAGATGATCCCGATAT pLKO.1 1105 3UTR 100% 10.800 5.400 Y TRIM51 n/a
10 TRCN0000237917 CCCTCAAGATGATCCCGATAT pLKO_005 1105 3UTR 100% 10.800 5.400 Y TRIM51EP n/a
11 TRCN0000236712 GGGATGCACAGAGAGACAAAG pLKO_005 370 3UTR 100% 10.800 5.400 Y TRIM51BP n/a
12 TRCN0000034014 CCTGCATTTCTCCATGAAGAA pLKO.1 649 3UTR 100% 4.950 2.475 Y TRIM51 n/a
13 TRCN0000033922 CATCTGCATGAACTACTTCAT pLKO.1 138 3UTR 100% 0.495 0.248 Y TRIM48 n/a
14 TRCN0000237915 TACCAGCACAGTAGGATTATT pLKO_005 1378 3UTR 100% 0.000 0.000 Y TRIM51EP n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_146921.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15934 pDONR223 0% 68.6% None (many diffs) n/a
2 ccsbBroad304_15934 pLX_304 0% 68.6% V5 (many diffs) n/a
3 TRCN0000476541 TCCAAACTTGTCGCGCACACCGTT pLX_317 25.8% 68.6% V5 (many diffs) n/a
4 ccsbBroadEn_03779 pDONR223 100% 68.6% None (many diffs) n/a
5 ccsbBroad304_03779 pLX_304 0% 68.6% V5 (many diffs) n/a
6 TRCN0000479767 CGAAGACGCCTACTTGGGTCTACC pLX_317 25.8% 68.6% V5 (many diffs) n/a
7 ccsbBroadEn_12855 pDONR223 100% 50.5% None (many diffs) n/a
8 ccsbBroad304_12855 pLX_304 0% 50.5% V5 (many diffs) n/a
9 TRCN0000480296 TGTATGCGTTACCGACTAGGCAAG pLX_317 52.1% 50.5% V5 (many diffs) n/a
10 ccsbBroadEn_12545 pDONR223 100% 32.7% None (many diffs) n/a
11 ccsbBroad304_12545 pLX_304 0% 32.7% V5 (many diffs) n/a
12 TRCN0000474512 GGGAGGGCCCAAAGGTCCATTTTA pLX_317 79.8% 32.7% V5 (many diffs) n/a
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