Construct: ORF TRCN0000480308
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019037.2_s317c1
- Derived from:
- ccsbBroadEn_14645
- DNA Barcode:
- CTTATTGTCCGCAGCATTACACCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FLT4 (2324)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480308
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2324 | FLT4 | fms related tyrosine kinase 4 | NM_002020.5 | 99.9% | 100% | 3894delG |
| 2 | human | 2324 | FLT4 | fms related tyrosine kinase 4 | NM_001354989.2 | 99.3% | 99.3% | 3894_3918del |
| 3 | human | 2324 | FLT4 | fms related tyrosine kinase 4 | NM_182925.5 | 95.2% | 95.1% | 3894_4089del |
| 4 | human | 2324 | FLT4 | fms related tyrosine kinase 4 | XM_017009266.1 | 93.7% | 92.8% | (many diffs) |
| 5 | human | 2324 | FLT4 | fms related tyrosine kinase 4 | XM_017009267.2 | 93.7% | 92.8% | (many diffs) |
| 6 | human | 2324 | FLT4 | fms related tyrosine kinase 4 | XM_017009268.1 | 93.2% | 93.2% | 0_1ins78;3816_4011del |
| 7 | human | 2324 | FLT4 | fms related tyrosine kinase 4 | XM_017009263.1 | 91.1% | 90% | (many diffs) |
| 8 | human | 2324 | FLT4 | fms related tyrosine kinase 4 | XM_017009264.2 | 91.1% | 90% | (many diffs) |
| 9 | human | 2324 | FLT4 | fms related tyrosine kinase 4 | XM_017009265.1 | 91.1% | 90% | (many diffs) |
| 10 | human | 2324 | FLT4 | fms related tyrosine kinase 4 | XM_011534478.3 | 89.5% | 88.5% | (many diffs) |
| 11 | human | 2324 | FLT4 | fms related tyrosine kinase 4 | XM_011534484.2 | 83.9% | 83.9% | 0_1ins459;3435_3630del |
| 12 | human | 2324 | FLT4 | fms related tyrosine kinase 4 | XR_001742050.2 | 61.7% | (many diffs) | |
| 13 | mouse | 14257 | Flt4 | FMS-like tyrosine kinase 4 | NM_008029.3 | 81.5% | 84.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3963
- ORF length:
- 3894
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcagcggggc gccgcgctgt gcctgcgact gtggctctgc ctgggactcc 121 tggacggcct ggtgagtggc tactccatga cccccccgac cttgaacatc acggaggagt 181 cacacgtcat cgacaccggt gacagcctgt ccatctcctg caggggacag caccccctcg 241 agtgggcttg gccaggagct caggaggcgc cagccaccgg agacaaggac agcgaggaca 301 cgggggtggt gcgagactgc gagggcacag acgccaggcc ctactgcaag gtgttgctgc 361 tgcacgaggt acatgccaac gacacaggca gctacgtctg ctactacaag tacatcaagg 421 cacgcatcga gggcaccacg gccgccagct cctacgtgtt cgtgagagac tttgagcagc 481 cattcatcaa caagcctgac acgctcttgg tcaacaggaa ggacgccatg tgggtgccct 541 gtctggtgtc catccccggc ctcaatgtca cgctgcgctc gcaaagctcg gtgctgtggc 601 cagacgggca ggaggtggtg tgggatgacc ggcggggcat gctcgtgtcc acgccactgc 661 tgcacgatgc cctgtacctg cagtgcgaga ccacctgggg agaccaggac ttcctttcca 721 accccttcct ggtgcacatc acaggcaacg agctctatga catccagctg ttgcccagga 781 agtcgctgga gctgctggta ggggagaagc tggtcctgaa ctgcaccgtg tgggctgagt 841 ttaactcagg tgtcaccttt gactgggact acccagggaa gcaggcagag cggggtaagt 901 gggtgcccga gcgacgctcc cagcagaccc acacagaact ctccagcatc ctgaccatcc 961 acaacgtcag ccagcacgac ctgggctcgt atgtgtgcaa ggccaacaac ggcatccagc 1021 gatttcggga gagcaccgag gtcattgtgc atgaaaatcc cttcatcagc gtcgagtggc 1081 tcaaaggacc catcctggag gccacggcag gagacgagct ggtgaagctg cccgtgaagc 1141 tggcagcgta ccccccgccc gagttccagt ggtacaagga tggaaaggca ctgtccgggc 1201 gccacagtcc acatgccctg gtgctcaagg aggtgacaga ggccagcaca ggcacctaca 1261 ccctcgccct gtggaactcc gctgctggcc tgaggcgcaa catcagcctg gagctggtgg 1321 tgaatgtgcc cccccagata catgagaagg aggcctcctc ccccagcatc tactcgcgtc 1381 acagccgcca ggccctcacc tgcacggcct acggggtgcc cctgcctctc agcatccagt 1441 ggcactggcg gccctggaca ccctgcaaga tgtttgccca gcgtagtctc cggcggcggc 1501 agcagcaaga cctcatgcca cagtgccgtg actggagggc ggtgaccacg caggatgccg 1561 tgaaccccat cgagagcctg gacacctgga ccgagtttgt ggagggaaag aataagactg 1621 tgagcaagct ggtgatccag aatgccaacg tgtctgccat gtacaagtgt gtggtctcca 1681 acaaggtggg ccaggatgag cggctcatct acttctatgt gaccaccatc cccgacggct 1741 tcaccatcga atccaagcca tccgaggagc tactagaggg ccagccggtg ctcctgagct 1801 gccaagccga cagctacaag tacgagcatc tgcgctggta ccgcctcaac ctgtccacgc 1861 tgcacgatgc gcacgggaac ccgcttctgc tcgactgcaa gaacgtgcat ctgttcgcca 1921 cccctctggc cgccagcctg gaggaggtgg cacctggggc gcgccacgcc acgctcagcc 1981 tgagtatccc ccgcgtcgcg cccgagcacg agggccacta tgtgtgcgaa gtgcaagacc 2041 ggcgcagcca tgacaagcac tgccacaaga agtacctgtc ggtgcaggcc ctggaagccc 2101 ctcggctcac gcagaacttg accgacctcc tggtgaacgt gagcgactcg ctggagatgc 2161 agtgcttggt ggccggagcg cacgcgccca gcatcgtgtg gtacaaagac gagaggctgc 2221 tggaggaaaa gtctggagtc gacttggcgg actccaacca gaagctgagc atccagcgcg 2281 tgcgcgagga ggatgcggga cgctatctgt gcagcgtgtg caacgccaag ggctgcgtca 2341 actcctccgc cagcgtggcc gtggaaggct ccgaggataa gggcagcatg gagatcgtga 2401 tccttgtcgg taccggcgtc atcgctgtct tcttctgggt cctcctcctc ctcatcttct 2461 gtaacatgag gaggccggcc cacgcagaca tcaagacggg ctacctgtcc atcatcatgg 2521 accccgggga ggtgcctctg gaggagcaat gcgaatacct gtcctacgat gccagccagt 2581 gggaattccc ccgagagcgg ctgcacctgg ggagagtgct cggctacggc gccttcggga 2641 aggtggtgga agcctccgct ttcggcatcc acaagggcag cagctgtgac accgtggccg 2701 tgaaaatgct gaaagagggc gccacggcca gcgagcaccg cgcgctgatg tcggagctca 2761 agatcctcat tcacatcggc aaccacctca acgtggtcaa cctcctcggg gcgtgcacca 2821 agccgcaggg ccccctcatg gtgatcgtgg agttctgcaa gtacggcaac ctctccaact 2881 tcctgcgcgc caagcgggac gccttcagcc cctgcgcgga gaagtctccc gagcagcgcg 2941 gacgcttccg cgccatggtg gagctcgcca ggctggatcg gaggcggccg gggagcagcg 3001 acagggtcct cttcgcgcgg ttctcgaaga ccgagggcgg agcgaggcgg gcttctccag 3061 accaagaagc tgaggacctg tggctgagcc cgctgaccat ggaagatctt gtctgctaca 3121 gcttccaggt ggccagaggg atggagttcc tggcttcccg aaagtgcatc cacagagacc 3181 tggctgctcg gaacattctg ctgtcggaaa gcgacgtggt gaagatctgt gactttggcc 3241 ttgcccggga catctacaaa gaccccgact acgtccgcaa gggcagtgcc cggctgcccc 3301 tgaagtggat ggcccctgaa agcatcttcg acaaggtgta caccacgcag agtgacgtgt 3361 ggtcctttgg ggtgcttctc tgggagatct tctctctggg ggcctccccg taccctgggg 3421 tgcagatcaa tgaggagttc tgccagcggc tgagagacgg cacaaggatg agggccccgg 3481 agctggccac tcccgccata cgccgcatca tgctgaactg ctggtccgga gaccccaagg 3541 cgagacctgc attctcggag ctggtggaga tcctggggga ccTGCTCCAG GGCAGGGGCC 3601 TGCAAGAGGA AGAGGAGGTC TGCATGGCCC CGCGCAGCTC TCAGAGCTCA GAAGAGGGCA 3661 GCTTCTCGCA GGTGTCCACC ATGGCCCTAC ACATCGCCCA GGCTGACGCT GAGGACAGCC 3721 CGCCAAGCCT GCAGCGCCAC AGCCTGGCCG CCAGGTATTA CAACTGGGTG TCCTTTCCCG 3781 GGTGCCTGGC CAGAGGGGCT GAGACCCGTG GTTCCTCCAG GATGAAGACA TTTGAGGAAT 3841 TCCCCATGAC CCCAACGACC TACAAAGGCT CTGTGGACAA CCAGACAGAC AGTGGGATGG 3901 TGCTGGCCTC GGAGGAGTTT GAGCAGATAG AGAGCAGGCA TAGACAAGAA AGCGGCTTCA 3961 GGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 4021 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 4081 TTTATATATC TTGTGGAAAG GACGACTTAT TGTCCGCAGC ATTACACCCA CGCGTTAAGT 4141 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt