Construct: ORF TRCN0000480316
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016228.2_s317c1
- Derived from:
- ccsbBroadEn_15354
- DNA Barcode:
- TGGCCCATCAAACGAGCCTTATTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AKR1B1 (231)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480316
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 231 | AKR1B1 | aldo-keto reductase family ... | NM_001628.4 | 99.7% | 99.6% | 6A>T;424T>C |
| 2 | human | 231 | AKR1B1 | aldo-keto reductase family ... | NM_001346142.1 | 54.4% | 54.4% | 0_1ins432 |
| 3 | human | 231 | AKR1B1 | aldo-keto reductase family ... | XM_005250234.3 | 54.4% | 54.4% | 0_1ins432 |
| 4 | human | 231 | AKR1B1 | aldo-keto reductase family ... | XM_024446695.1 | 54.4% | 54.4% | 0_1ins432 |
| 5 | human | 231 | AKR1B1 | aldo-keto reductase family ... | NR_144376.1 | 45.5% | (many diffs) | |
| 6 | mouse | 11677 | Akr1b3 | aldo-keto reductase family ... | NM_009658.3 | 85.6% | 85.1% | (many diffs) |
| 7 | mouse | 626009 | Gm6644 | predicted gene 6644; Akr1b3... | NR_037965.1 | 59.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1014
- ORF length:
- 948
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tagccgtctc ctgctcaaca acggcgccaa gatgcccatc ctggggttgg 121 gtacctggaa gtcccctcca gggcaggtga ctgaggccgt gaaggtggcc attgacgtcg 181 ggtaccgcca catcgactgt gcccatgtgt accagaatga gaatgaggtg ggggtggcca 241 ttcaggagaa gctcagggag caggtggtga agcgtgagga gctcttcatc gtcagcaagc 301 tgtggtgcac gtaccatgag aagggcctgg tgaaaggagc ctgccagaag acactcagcg 361 acctgaagct ggactacctg gacctctacc ttattcactg gccgactggc tttaagcctg 421 ggaaggaatt tttcccattg gatgagtcgg gcaatgtggt tcccagtgac accaacattc 481 tggacacgcg ggcggccatg gaagagctgg tggatgaagg gctggtgaaa gctattggca 541 tctccaactt caaccaTCTC CAGGTGGAGA TGATCTTAAA CAAACCTGGC TTGAAGTATA 601 AGCCTGCAGT TAACCAGATT GAGTGCCACC CATATCTCAC TCAGGAGAAG TTAATCCAGT 661 ACTGCCAGTC CAAAGGCATC GTGGTGACCG CCTACAGCCC CCTCGGCTCT CCTGACAGGC 721 CCTGGGCCAA GCCCGAGGAC CCTTCTCTCC TGGAGGATCC CAGGATCAAG GCGATCGCAG 781 CCAAGCACAA TAAAACTACA GCCCAGGTCC TGATCCGGTT CCCCATGCAG AGGAACTTGG 841 TGGTGATCCC CAAGTCTGTG ACACCAGAAC GCATTGCTGA GAACTTTAAG GTCTTTGACT 901 TTGAACTGAG CAGCCAGGAT ATGACCACCT TACTCAGCTA CAACAGGAAC TGGAGGGTCT 961 GTGCCTTGTT GAGCTGTACC TCCCACAAGG ATTACCCCTT CCATGAAGAG TTTCGCCCAA 1021 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1081 ATTCTACGTA GTAATGAACT AGCCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1141 TATCTTGTGG AAAGGACGAT GGCCCATCAA ACGAGCCTTA TTTACGCGTT AAGTCgacaa 1201 tcaacctctg gattacaaaa tttgtgaaag att