Construct: ORF TRCN0000480318
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015353.1_s317c1
- Derived from:
- ccsbBroadEn_15875
- DNA Barcode:
- ACTCGGGTCACATCCTCTCAATCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NSD3 (54904)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480318
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54904 | NSD3 | nuclear receptor binding SE... | NM_017778.2 | 100% | 100% | |
| 2 | human | 54904 | NSD3 | nuclear receptor binding SE... | NM_023034.2 | 44.3% | 43.3% | (many diffs) |
| 3 | mouse | 234135 | Nsd3 | nuclear receptor binding SE... | NM_001001735.2 | 88.5% | 91.6% | (many diffs) |
| 4 | mouse | 234135 | Nsd3 | nuclear receptor binding SE... | NM_001308481.1 | 88.5% | 91.6% | (many diffs) |
| 5 | mouse | 234135 | Nsd3 | nuclear receptor binding SE... | NM_001308482.1 | 83.8% | 85.6% | (many diffs) |
| 6 | mouse | 234135 | Nsd3 | nuclear receptor binding SE... | XM_017312681.1 | 41.3% | 41.7% | (many diffs) |
| 7 | mouse | 234135 | Nsd3 | nuclear receptor binding SE... | XM_017312680.1 | 41% | 41.4% | (many diffs) |
| 8 | mouse | 234135 | Nsd3 | nuclear receptor binding SE... | XM_017312678.1 | 39.9% | 40.3% | (many diffs) |
| 9 | mouse | 234135 | Nsd3 | nuclear receptor binding SE... | NM_001081269.2 | 39.6% | 40% | (many diffs) |
| 10 | mouse | 234135 | Nsd3 | nuclear receptor binding SE... | XM_017312676.1 | 39.6% | 40% | (many diffs) |
| 11 | mouse | 234135 | Nsd3 | nuclear receptor binding SE... | XM_017312677.1 | 39.6% | 40% | (many diffs) |
| 12 | mouse | 234135 | Nsd3 | nuclear receptor binding SE... | XM_017312679.1 | 38.2% | 38.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2001
- ORF length:
- 1935
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tttctctttc tctttcatgc aagggatcat gggaaacaca attcagcaac 121 cacctcaact cattgactcc gccaacatcc gtcaggagga tgcctttgat aacaacagtg 181 acattgctga agatggtggc cagacaccat atgaagctac tttgcagcaa ggctttcagt 241 acccagctac aacagaagat cttcctccac tcacaaatgg gtatccatca tcaatcagtg 301 tgtatgaaac tcaaaccaaa taccagtcat ataatcagta tcctaatggg tcagccaatg 361 gctttggtgc agttagaaac tttagcccca ctgactatta tcattcagaa attccaaaca 421 caagaccaca tgaaattctg gaaaaacctt cccctccaca gccaccacct cctccttcgg 481 taccacaaac tgtgattcca aagaagactg gctcacctga aattaaacta aaaataacca 541 aaactatcca gaatggcagg gaattgtttg agtcttccct ttgtggagac cttttaaatg 601 aagtacaggc aagtgagcac acgaaatcaa agcatgaaag cagaaaagaa aagaggaaaa 661 aaagcaacaa gcatgactca tcaagatctg aagagcgcaa gtcacacaaa atccccaaat 721 tagaaccaga ggaacaaaat agaccaaatg agagggttga cactgtatca gaaaaaccaa 781 gggaagaacc agtactaaaa gaggaagccc cagttcagcc aatactatct tctgttccaa 841 caacggaagt gtccactggt gttaagtttc aggttggcga tcttgtgtgg tccaaggtgg 901 gaacctatcc ttggtggcct tgtatggttt caagtgatcc ccagcttgag gttcatacta 961 aaattaacac aagaggtgcc cgagaatatc atgtccagtt ttttagcaac cagccagaga 1021 gggcgtgggt tcatgaaaaa cgggtacgag agtataaagg tcataaacag tatgaagaat 1081 tactggctga ggcaaccaaa caagccagca atcactctga gaaacaaaag attcggaaac 1141 cccgacctca gagagaacgt gctcagtggg atattggcat tgcccatgca gagaaagcat 1201 tgaaaatgac tcgagaagaa agaatagaac agtatacttt tatttacatt gataaacagc 1261 ctgaagaggc tttatcccaa gcaaaaaaga gtgttgcctc caaaaccgaa gttaaaaaaa 1321 cccgacgacc aagatctgtg ctgaatactc agccagaaca gaccaatgca ggggaggtgg 1381 cctcctcact ctcaagtact gaaattcgga gacatagcca gaggcggcac acaagtgcgg 1441 aagaggaaga gccaccgcct gttaaaatag cctggaaaac tgcggcagca aggaaatcct 1501 taccagcttc cattacgatg cacaaaggga gcctGGATTT GCAGAAGTGT AACATGTCTC 1561 CAGTTGTGAA AATTGAACAA GTGTTTGCTC TTCAGAATGC TACAGGGGAT GGGAAATTTA 1621 TCGATCAATT TGTTTATTCA ACAAAGGGAA TTGGTAACAA AACAGAAATA AGTGTCAGGG 1681 GGCAAGACAG GCTTATAATT TCTACACCAA ACCAGAGAAA TGAAAAGCCA ACGCAGAGTG 1741 TATCATCTCC TGAAGCAACA TCTGGTTCTA CAGGCTCAGT AGAAAAGAAG CAACAGAGAA 1801 GATCAATTAG AACTCGTTCT GAATCAGAGA AATCCACTGA GGTTGTGCCA AAGAAGAAGA 1861 TCAAAAAGGA GCAGGTTGAA ACAGTTCCTC AGGCTACAGT GAAGACTGGA TTACAGAAAG 1921 GGTCGGCGGA CCGGGGAGTG CAGGGCTCTG TCAGATTCAG TGACAGCTCC GTCTCCGCAG 1981 CGATTGAGGA AACTGTGGAC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 2041 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2101 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACTC GGGTCACATC 2161 CTCTCAATCT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt