Construct: ORF TRCN0000480323
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011899.1_s317c1
- Derived from:
- ccsbBroadEn_02347
- DNA Barcode:
- GCCTTACGCATACAACGGAACCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALG3 (10195)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480323
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10195 | ALG3 | ALG3 alpha-1,3- mannosyltra... | NM_005787.6 | 100% | 100% | |
| 2 | human | 10195 | ALG3 | ALG3 alpha-1,3- mannosyltra... | XM_011512323.2 | 88.6% | 86% | (many diffs) |
| 3 | human | 10195 | ALG3 | ALG3 alpha-1,3- mannosyltra... | NM_001006941.2 | 88.2% | 81.9% | (many diffs) |
| 4 | human | 10195 | ALG3 | ALG3 alpha-1,3- mannosyltra... | XM_011512322.1 | 87.6% | 83.8% | (many diffs) |
| 5 | human | 10195 | ALG3 | ALG3 alpha-1,3- mannosyltra... | XM_024453296.1 | 83.1% | 83.1% | 0_1ins222 |
| 6 | human | 10195 | ALG3 | ALG3 alpha-1,3- mannosyltra... | NR_024534.1 | 82.3% | 1_31del;190_191ins37;1309_1513del | |
| 7 | human | 10195 | ALG3 | ALG3 alpha-1,3- mannosyltra... | NR_024533.1 | 78.3% | 1_31del;226_227ins100;1246_1450del | |
| 8 | mouse | 208624 | Alg3 | asparagine-linked glycosyla... | NM_145939.2 | 86.8% | 88.8% | (many diffs) |
| 9 | mouse | 208624 | Alg3 | asparagine-linked glycosyla... | XM_006521932.3 | 79.9% | 76% | (many diffs) |
| 10 | mouse | 208624 | Alg3 | asparagine-linked glycosyla... | XM_006521933.3 | 63.2% | 65.2% | (many diffs) |
| 11 | mouse | 208624 | Alg3 | asparagine-linked glycosyla... | XM_006521934.3 | 45.8% | 47.4% | (many diffs) |
| 12 | mouse | 208624 | Alg3 | asparagine-linked glycosyla... | XM_017316917.1 | 45.8% | 47.4% | (many diffs) |
| 13 | mouse | 208624 | Alg3 | asparagine-linked glycosyla... | XM_017316918.1 | 45.8% | 47.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1380
- ORF length:
- 1314
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggctgggctg cggaaacgcg gccggtccgg ttccgcggcc caggcagagg 121 gactctgcaa gcaatggctg cagcgcgcct ggcaagagcg gcgcctgctg ctgcgggagc 181 cgcgctacac gctgctggtg gccgcctgcc tctgcctggc ggaggtgggc atcaccttct 241 gggtcattca cagggtggca tacacagaga ttgactggaa ggcctacatg gccgaggtag 301 aaggcgtcat caatggtacc tatgactata cccaactgca gggtgacacc ggaccacttg 361 tgtacccagc tggtttcgtg tacatcttta tggggttgta ctatgccacc agccgaggca 421 ctgacatccg catggcccag aacatctttg ctgtgctcta cctggctacc ttgctgcttg 481 tcttcttgat ctatcaccag acctgcaagg tacctccctt cgtctttttc ttcatgtgct 541 gcgcctctta ccgtgtccac tccatctttg tgctgcggct cttcaatgac ccagtggcca 601 tggtgctgct cttcctcagt atcaacctcc tgctggccca gcgctggggc tggggttgct 661 gctttttcag cctggcagtc tctgtgaaga tgaatgtgct gctcttcgcc cctgggttac 721 tgtttcttct cctcacacag tttggcttcc gtggggccct ccccaagctg ggaatctgtg 781 ctggccttca ggtggtgctg gggctgccct tcctgctgga gaaccccagc ggctacctgt 841 cccgctcctt tgaccttggc cgccagtttc tgttccactg gacagtgaac tggcgcttcc 901 tcccagaggc gctcttcctg catcgagcct tccacctggc cctgttgact gcccacctca 961 ccctgctcct gctgtttgcc ctctgcaggt ggcacaggac aggGGAAAGT ATCTTGTCGC 1021 TGCTGAGGGA TCCCTCCAAA AGGAAGGTTC CACCCCAGCC CCTTACACCC AACCAGATCG 1081 TTTCTACCCT CTTCACCTCC AACTTCATTG GCATCTGCTT CAGCCGCTCC CTCCACTACC 1141 AGTTCTACGT CTGGTATTTC CACACACTGC CCTACCTCCT GTGGGCCATG CCTGCACGCT 1201 GGCTCACACA CCTGCTCAGG TTGTTGGTGC TGGGGCTCAT CGAGCTCTCC TGGAACACAT 1261 ACCCTTCCAC ATCCTGCAGC TCTGCTGCCC TGCACATATG CCATGCCGTC ATCCTGCTGC 1321 AGCTCTGGCT GGGCCCGCAG CCTTTCCCCA AGAGCACCCA ACACAGCAAG AAAGCCCACT 1381 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1441 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1501 TTTATATATC TTGTGGAAAG GACGAGCCTT ACGCATACAA CGGAACCCGA CGCGTTAAGT 1561 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt