Construct: ORF TRCN0000480323

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011899.1_s317c1
Derived from:
ccsbBroadEn_02347
DNA Barcode:
GCCTTACGCATACAACGGAACCCG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ALG3 (10195)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480323
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10195 ALG3 ALG3 alpha-1,3- mannosyltra... NM_005787.6 100% 100%
2 human 10195 ALG3 ALG3 alpha-1,3- mannosyltra... XM_011512323.2 88.6% 86% (many diffs)
3 human 10195 ALG3 ALG3 alpha-1,3- mannosyltra... NM_001006941.2 88.2% 81.9% (many diffs)
4 human 10195 ALG3 ALG3 alpha-1,3- mannosyltra... XM_011512322.1 87.6% 83.8% (many diffs)
5 human 10195 ALG3 ALG3 alpha-1,3- mannosyltra... XM_024453296.1 83.1% 83.1% 0_1ins222
6 human 10195 ALG3 ALG3 alpha-1,3- mannosyltra... NR_024534.1 82.3% 1_31del;190_191ins37;1309_1513del
7 human 10195 ALG3 ALG3 alpha-1,3- mannosyltra... NR_024533.1 78.3% 1_31del;226_227ins100;1246_1450del
8 mouse 208624 Alg3 asparagine-linked glycosyla... NM_145939.2 86.8% 88.8% (many diffs)
9 mouse 208624 Alg3 asparagine-linked glycosyla... XM_006521932.3 79.9% 76% (many diffs)
10 mouse 208624 Alg3 asparagine-linked glycosyla... XM_006521933.3 63.2% 65.2% (many diffs)
11 mouse 208624 Alg3 asparagine-linked glycosyla... XM_006521934.3 45.8% 47.4% (many diffs)
12 mouse 208624 Alg3 asparagine-linked glycosyla... XM_017316917.1 45.8% 47.4% (many diffs)
13 mouse 208624 Alg3 asparagine-linked glycosyla... XM_017316918.1 45.8% 47.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1380
ORF length:
1314
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc ggctgggctg cggaaacgcg gccggtccgg ttccgcggcc caggcagagg
121gactctgcaa gcaatggctg cagcgcgcct ggcaagagcg gcgcctgctg ctgcgggagc
181cgcgctacac gctgctggtg gccgcctgcc tctgcctggc ggaggtgggc atcaccttct
241gggtcattca cagggtggca tacacagaga ttgactggaa ggcctacatg gccgaggtag
301aaggcgtcat caatggtacc tatgactata cccaactgca gggtgacacc ggaccacttg
361tgtacccagc tggtttcgtg tacatcttta tggggttgta ctatgccacc agccgaggca
421ctgacatccg catggcccag aacatctttg ctgtgctcta cctggctacc ttgctgcttg
481tcttcttgat ctatcaccag acctgcaagg tacctccctt cgtctttttc ttcatgtgct
541gcgcctctta ccgtgtccac tccatctttg tgctgcggct cttcaatgac ccagtggcca
601tggtgctgct cttcctcagt atcaacctcc tgctggccca gcgctggggc tggggttgct
661gctttttcag cctggcagtc tctgtgaaga tgaatgtgct gctcttcgcc cctgggttac
721tgtttcttct cctcacacag tttggcttcc gtggggccct ccccaagctg ggaatctgtg
781ctggccttca ggtggtgctg gggctgccct tcctgctgga gaaccccagc ggctacctgt
841cccgctcctt tgaccttggc cgccagtttc tgttccactg gacagtgaac tggcgcttcc
901tcccagaggc gctcttcctg catcgagcct tccacctggc cctgttgact gcccacctca
961ccctgctcct gctgtttgcc ctctgcaggt ggcacaggac aggGGAAAGT ATCTTGTCGC
1021TGCTGAGGGA TCCCTCCAAA AGGAAGGTTC CACCCCAGCC CCTTACACCC AACCAGATCG
1081TTTCTACCCT CTTCACCTCC AACTTCATTG GCATCTGCTT CAGCCGCTCC CTCCACTACC
1141AGTTCTACGT CTGGTATTTC CACACACTGC CCTACCTCCT GTGGGCCATG CCTGCACGCT
1201GGCTCACACA CCTGCTCAGG TTGTTGGTGC TGGGGCTCAT CGAGCTCTCC TGGAACACAT
1261ACCCTTCCAC ATCCTGCAGC TCTGCTGCCC TGCACATATG CCATGCCGTC ATCCTGCTGC
1321AGCTCTGGCT GGGCCCGCAG CCTTTCCCCA AGAGCACCCA ACACAGCAAG AAAGCCCACT
1381GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
1441GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
1501TTTATATATC TTGTGGAAAG GACGAGCCTT ACGCATACAA CGGAACCCGA CGCGTTAAGT
1561Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)