Transcript: Human XM_011512323.2

PREDICTED: Homo sapiens ALG3 alpha-1,3- mannosyltransferase (ALG3), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ALG3 (10195)
Length:
1402
CDS:
23..1219

Additional Resources:

NCBI RefSeq record:
XM_011512323.2
NBCI Gene record:
ALG3 (10195)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011512323.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000035444 CCAGATCGTTTCTACCCTCTT pLKO.1 910 CDS 100% 4.050 5.670 N ALG3 n/a
2 TRCN0000288750 CCAGATCGTTTCTACCCTCTT pLKO_005 910 CDS 100% 4.050 5.670 N ALG3 n/a
3 TRCN0000249393 TCTTCACCTCCAACTTCATTG pLKO_005 927 CDS 100% 10.800 7.560 N Alg3 n/a
4 TRCN0000035446 GCTGGTTTCGTGTACATCTTT pLKO.1 206 CDS 100% 5.625 3.938 N ALG3 n/a
5 TRCN0000288751 GCTGGTTTCGTGTACATCTTT pLKO_005 206 CDS 100% 5.625 3.938 N ALG3 n/a
6 TRCN0000035445 GCTGCTTGTCTTCTTGATCTA pLKO.1 310 CDS 100% 4.950 3.465 N ALG3 n/a
7 TRCN0000288752 GCTGCTTGTCTTCTTGATCTA pLKO_005 310 CDS 100% 4.950 3.465 N ALG3 n/a
8 TRCN0000035447 CTACCAGTTCTACGTCTGGTA pLKO.1 973 CDS 100% 0.264 0.185 N ALG3 n/a
9 TRCN0000288824 CTACCAGTTCTACGTCTGGTA pLKO_005 973 CDS 100% 0.264 0.185 N ALG3 n/a
10 TRCN0000035448 CCTGGCAGTCTCTGTGAAGAT pLKO.1 508 CDS 100% 4.950 2.970 N ALG3 n/a
11 TRCN0000288749 CCTGGCAGTCTCTGTGAAGAT pLKO_005 508 CDS 100% 4.950 2.970 N ALG3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011512323.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02347 pDONR223 100% 88.6% 86% None (many diffs) n/a
2 ccsbBroad304_02347 pLX_304 0% 88.6% 86% V5 (many diffs) n/a
3 TRCN0000480323 GCCTTACGCATACAACGGAACCCG pLX_317 29% 88.6% 86% V5 (many diffs) n/a
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