Construct: ORF TRCN0000480325
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011491.2_s317c1
- Derived from:
- ccsbBroadEn_08681
- DNA Barcode:
- CATAAGCAAGGTCTTTGGAAAGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COQ8A (56997)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480325
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56997 | COQ8A | coenzyme Q8A | NM_020247.5 | 99.9% | 100% | 1185C>T |
| 2 | human | 56997 | COQ8A | coenzyme Q8A | XM_005273201.1 | 99.9% | 100% | 1185C>T |
| 3 | human | 56997 | COQ8A | coenzyme Q8A | XM_011544238.1 | 99.9% | 100% | 1185C>T |
| 4 | human | 56997 | COQ8A | coenzyme Q8A | XM_011544239.2 | 99.9% | 100% | 1185C>T |
| 5 | human | 56997 | COQ8A | coenzyme Q8A | XM_011544241.2 | 99.9% | 100% | 1185C>T |
| 6 | human | 56997 | COQ8A | coenzyme Q8A | XM_017001852.1 | 99.9% | 100% | 1185C>T |
| 7 | human | 56997 | COQ8A | coenzyme Q8A | XM_024448517.1 | 99.9% | 100% | 1185C>T |
| 8 | human | 56997 | COQ8A | coenzyme Q8A | XM_024448518.1 | 99.9% | 100% | 1185C>T |
| 9 | mouse | 67426 | Coq8a | coenzyme Q8A | NM_001163290.1 | 83.4% | 86.5% | (many diffs) |
| 10 | mouse | 67426 | Coq8a | coenzyme Q8A | NM_023341.3 | 83.4% | 86.5% | (many diffs) |
| 11 | mouse | 67426 | Coq8a | coenzyme Q8A | XM_006496961.2 | 83.4% | 86.5% | (many diffs) |
| 12 | mouse | 67426 | Coq8a | coenzyme Q8A | XM_006496962.2 | 83.4% | 86.5% | (many diffs) |
| 13 | mouse | 67426 | Coq8a | coenzyme Q8A | XM_006496963.3 | 77.8% | 79.9% | (many diffs) |
| 14 | mouse | 67426 | Coq8a | coenzyme Q8A | XM_011238855.2 | 67.2% | 71.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2007
- ORF length:
- 1941
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgccatattg ggagacacca tcatggtggc taaaggcctt gtcaagctga 121 cccaggcggc cgtggaaacc cacctgcagc acttgggcat cggaggggag ctgatcatgg 181 cggccagggc cctgcagtcc acggctgtgg agcagattgg catgttcttg gggaaggtgc 241 agggtcagga taaacatgaa gaatattttg ctgagaactt cggcggccca gaaggggagt 301 tccacttctc agtcccgcat gcagccggag cctccacaga cttctcttca gcctccgctc 361 ccgaccagtc agcgccccca tccctgggtc atgcccacag cgagggccca gctcctgcct 421 acgtggccag tggacccttt agagaagccg ggttccccgg ccaggcctcc tcccctctgg 481 gcagggccaa cgggaggctc tttgcaaacc ccagagactc attctctgcc atgggctttc 541 agcgaaggtt cttccaccag gaccaatccc ctgttggggg cctcacagcc gaggacattg 601 agaaggcccg gcaggctaag gctcgccccg agaacaagca gcacaaacag acgctcagcg 661 agcatgcccg ggagcggaag gtgcctgtga cgaggattgg ccggctggcc aacttcggag 721 gtctggccgt gggcctgggc ttcggggcac tggcagaggt cgccaagaag agcctgcgct 781 ccgaggaccc ctcagggaag aaggccgtgc tgggttccag tcctttcctg tccgaggcca 841 atgcagagcg gatcgtgcgc acgctctgca aggtgcgtgg tgcggcactc aagctgggcc 901 agatgctgag catccaggat gatgccttta tcaaccccca cctggctaag atcttcgagc 961 gggtgcggca gagcgcggac ttcatgccac tgaagcagat gatgaaaact ctcaacaacg 1021 acctgggccc caactggcgg gacaagttgg aatacttcga ggagcggccc ttcgccgccg 1081 catccattgg gcaggtgcac ttggcccgaa tgaagggcgg ccgcgaggtg gccatgaaga 1141 tccagtaccc tggcgtggcc cagagcatca acagtgatgt caacaacctc atggccgtgt 1201 tgaacatgag caacatgctt ccagaaggcc tgttccccga gcacctgatt gacgtgctga 1261 ggcgggagct ggccctggag tgtgactacc agcgagaggc cgcctgtgcc cgcaagttca 1321 gggacctgct gaagggccac cccttcttct atgtgcctga gattgtggat gagctctgca 1381 gcccacatgt gctgaccaca gagctggtgt ctggcttccc cctggaccag gccgaagggc 1441 tcagccagga gattcggaac gagatctgct acaacatcct ggttctgtgc ctgagggagc 1501 tgttcgagtt ccacttcatg caaacagacc ccaactggtc caacttcttc tatgaccccc 1561 agcagcacaa ggtggctcTT TTGGATTTTG GGGCAACGCG GGAATATGAC AGATCCTTCA 1621 CCGACCTCTA CATTCAGATC ATCAGGGCTG CTGCCGACAG GGACAGGGAG ACTGTGCGGG 1681 CGAAATCCAT AGAGATGAAG TTCCTCACCG GCTACGAGGT CAAGGTCATG GAAGACGCCC 1741 ACTTGGATGC CATCCTCATC CTGGGGGAGG CCTTCGCCTC TGATGAGCCT TTTGATTTTG 1801 GCACTCAGAG CACCACCGAG AAGATCCACA ACCTGATTCC CGTCATGCTG AGGCACCGTC 1861 TCGTCCCCCC ACCCGAGGAA ACCTACTCCC TGCACAGGAA GATGGGGGGC TCCTTCCTCA 1921 TCTGCTCCAA GCTGAAGGCC CGCTTCCCCT GCAAGGCCAT GTTCGAGGAG GCCTACAGCA 1981 ACTACTGCAA GAGGCAGGCC CAGCAGTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 2041 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2101 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACATAAGCA 2161 AGGTCTTTGG AAAGTCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2221 aagatt