Construct: ORF TRCN0000480330
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008403.1_s317c1
- Derived from:
- ccsbBroadEn_11623
- DNA Barcode:
- ACCTCTGGCGGGTTGGATCATTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACOT7 (11332)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480330
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11332 | ACOT7 | acyl-CoA thioesterase 7 | NM_181866.3 | 96.6% | 96.1% | (many diffs) |
| 2 | human | 11332 | ACOT7 | acyl-CoA thioesterase 7 | NM_181865.3 | 95.1% | 91.2% | (many diffs) |
| 3 | human | 11332 | ACOT7 | acyl-CoA thioesterase 7 | NM_007274.3 | 91.3% | 91.3% | 1_96del |
| 4 | human | 11332 | ACOT7 | acyl-CoA thioesterase 7 | NM_181864.3 | 87.5% | 86% | (many diffs) |
| 5 | human | 344967 | LOC344967 | acyl-CoA thioesterase 7 pse... | NR_027277.2 | 55.6% | (many diffs) | |
| 6 | mouse | 70025 | Acot7 | acyl-CoA thioesterase 7 | XM_006539169.2 | 85.1% | 92% | (many diffs) |
| 7 | mouse | 70025 | Acot7 | acyl-CoA thioesterase 7 | NM_001146058.1 | 83% | 89.4% | (many diffs) |
| 8 | mouse | 70025 | Acot7 | acyl-CoA thioesterase 7 | NM_133348.2 | 79.2% | 85.4% | (many diffs) |
| 9 | mouse | 70025 | Acot7 | acyl-CoA thioesterase 7 | NM_001146057.1 | 77.5% | 81.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1080
- ORF length:
- 1014
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc gggcccagac gtcgagacgc cgtccgccat ccagatctgc cggatcatgc 121 ggccagatga tgccaacgtg gccggcaatg tccacggggg gaccatcctg aagatgatcg 181 aggaggcagg cgccatcatc agcacccggc attgcaacag ccagaacggg gagcgctgtg 241 tggccgccct ggctcgtgtc gagcgcaccg acttcctgtc tcccatgtgc atcggtgagg 301 tggcgcatgt cagcgcggag atcacctaca cctccaagca ctctgtggag gtgcaggtca 361 acgtgatgtc cgaaaacatc ctcacaggtg ccaaaaagct gaccaataag gccaccctgt 421 ggtatgtgcc cctgtcgctg aagaatgtgg acaaggtcct cgaggtgcct cctgttgtgt 481 attcccggca ggagcaggag gaggagggcc ggaagcggta tgaagcccag aagctggagc 541 gcatggagac caagtggagg aacggggaca tcgtccagcc agtcctcaac ccagagccga 601 acactgtcag ctacagccag tccagcttga tccacctggt ggggccTTCA GACTGCACCC 661 TGCACGGCTT TGTGCACGGA GGTGTGACCA TGAAGCTCAT GGATGAGGTC GCCGGGATCG 721 TGGCTGCACG CCACTGCAAG ACCAACATCG TCACAGCTTC CGTGGACGCC ATTAATTTTC 781 ATGACAAGAT CAGAAAAGGC TGCGTCATCA CCATCTCGGG ACGCATGACC TTCACGAGCA 841 ATAAGTCCAT GGAGATCGAG GTGTTGGTGG ACGCCGACCC TGTTGTGGAC AGCTCTCAGA 901 AGCGCTACCG GGCCGCCAGT GCCTTCTTCA CCTACGTGTC GCTGAGCCAG GAAGGCAGGT 961 CGCTGCCTGT GCCCCAGCTG GTGCCCGAGA CCGAGGACGA GAAGAAGCGC TTTGAGGAAG 1021 GCAAAGGGCG GTACCTGCAG ATGAAGGCGA AGCGACAGGG CCACGCGGAG CCTCAGCCCT 1081 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1141 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1201 TTTATATATC TTGTGGAAAG GACGAACCTC TGGCGGGTTG GATCATTCGA CGCGTTAAGT 1261 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt