Construct: ORF TRCN0000480331

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007156.1_s317c1
Derived from:
ccsbBroadEn_03941
DNA Barcode:
TGTGCCTCATCCAGAACCACGTAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CHST8 (64377)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480331
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 64377 CHST8 carbohydrate sulfotransfera... NM_001127895.2 100% 100%
2 human 64377 CHST8 carbohydrate sulfotransfera... NM_001127896.2 100% 100%
3 human 64377 CHST8 carbohydrate sulfotransfera... NM_022467.3 100% 100%
4 human 64377 CHST8 carbohydrate sulfotransfera... XM_011527222.1 100% 100%
5 human 64377 CHST8 carbohydrate sulfotransfera... XM_011527224.1 100% 100%
6 human 64377 CHST8 carbohydrate sulfotransfera... XM_017027143.1 100% 100%
7 mouse 68947 Chst8 carbohydrate (N-acetylgalac... NM_175140.4 82% 85.6% (many diffs)
8 mouse 68947 Chst8 carbohydrate (N-acetylgalac... XM_006540337.3 82% 85.6% (many diffs)
9 mouse 68947 Chst8 carbohydrate (N-acetylgalac... XM_006540339.2 82% 85.6% (many diffs)
10 mouse 68947 Chst8 carbohydrate (N-acetylgalac... XM_006540340.2 82% 85.6% (many diffs)
11 mouse 68947 Chst8 carbohydrate (N-acetylgalac... XM_006540341.1 82% 85.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1338
ORF length:
1272
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgac cctgcgacct ggaacaatgc ggctggcctg catgttctct tccatcctgc
121tgttcggagc tgcaggcctc ctcctcttca tcagcctgca ggaccctacg gagctcgccc
181cccagcaggt gccaggaata aagttcaaca tcaggccaag gcagccccac cacgacctcc
241caccaggcgg ctcccaggat ggtgacttga aggaacccac agagagggtc actcgggact
301tatccagtgg ggccccgagg ggccgcaacc tgccagcgcc tgaccagcct caacccccgc
361tgcagagggg aacccgtctg cggctccgcc agcgccgtcg ccgtctgctc atcaagaaaa
421tgccagctgc ggcgaccatc ccggccaaca gctcggacgc gcccttcatc cggccgggac
481ccgggacgct ggatggccgc tgggtcagcc tgcaccggag ccagcaggag cgcaagcggg
541tgatgcagga ggcctgcgcc aagtaccggg cgagcagcag ccgccgggcc gtcacgcccc
601gccacgtgtc ccgtatcttc gtggaggacc gccaccgcgt gctctactgc gaggtgccca
661aggccggctg ctccaattgg aagcgggtgc tcatggtgct ggccggcctg gcctcgtcca
721ctgccgacat ccagcacaac accgtccact atggcagcgc tctcaagcgc ctggacacct
781tcgaccgcca gggtatcttg caccgtctca gcacctacac caagatgctc tttgtccgcg
841agcccttcga gaggctggtg tccgccttcc gcgacaagtt tgagcacccc aacagctact
901atcacccggt cttcggcaag gccatcctgg cccggtaccg cgccaatgcc tctcgggagg
961cccTGCGGAC CGGCTCTGGG GTGCGTTTTC CCGAGTTCGT CCAGTACCTG CTGGACGTGC
1021ACCGGCCCGT GGGGATGGAC ATTCACTGGG ACCATGTCAG CCGGCTCTGC AGCCCCTGCC
1081TCATCGACTA CGATTTCGTA GGCAAGTTCG AGAGCATGGA GGACGATGCC AACTTCTTCC
1141TGAGCCTCAT CCGCGCGCCG CGGAACCTGA CCTTCCCCCG GTTCAAGGAC CGGCACTCGC
1201AGGAGGCGCG GACCACAGCG AGGATCGCCC ACCAGTACTT CGCCCAACTC TCGGCCCTGC
1261AAAGGCAGCG CACCTACGAC TTCTACTACA TGGATTACCT GATGTTCAAC TATTCCAAGC
1321CCTTTGCAGA TCTGTACTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
1381TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
1441GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATGTGCCT CATCCAGAAC
1501CACGTATACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)

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