Construct: ORF TRCN0000480331
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007156.1_s317c1
- Derived from:
- ccsbBroadEn_03941
- DNA Barcode:
- TGTGCCTCATCCAGAACCACGTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHST8 (64377)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480331
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64377 | CHST8 | carbohydrate sulfotransfera... | NM_001127895.2 | 100% | 100% | |
| 2 | human | 64377 | CHST8 | carbohydrate sulfotransfera... | NM_001127896.2 | 100% | 100% | |
| 3 | human | 64377 | CHST8 | carbohydrate sulfotransfera... | NM_022467.3 | 100% | 100% | |
| 4 | human | 64377 | CHST8 | carbohydrate sulfotransfera... | XM_011527222.1 | 100% | 100% | |
| 5 | human | 64377 | CHST8 | carbohydrate sulfotransfera... | XM_011527224.1 | 100% | 100% | |
| 6 | human | 64377 | CHST8 | carbohydrate sulfotransfera... | XM_017027143.1 | 100% | 100% | |
| 7 | mouse | 68947 | Chst8 | carbohydrate (N-acetylgalac... | NM_175140.4 | 82% | 85.6% | (many diffs) |
| 8 | mouse | 68947 | Chst8 | carbohydrate (N-acetylgalac... | XM_006540337.3 | 82% | 85.6% | (many diffs) |
| 9 | mouse | 68947 | Chst8 | carbohydrate (N-acetylgalac... | XM_006540339.2 | 82% | 85.6% | (many diffs) |
| 10 | mouse | 68947 | Chst8 | carbohydrate (N-acetylgalac... | XM_006540340.2 | 82% | 85.6% | (many diffs) |
| 11 | mouse | 68947 | Chst8 | carbohydrate (N-acetylgalac... | XM_006540341.1 | 82% | 85.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1338
- ORF length:
- 1272
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac cctgcgacct ggaacaatgc ggctggcctg catgttctct tccatcctgc 121 tgttcggagc tgcaggcctc ctcctcttca tcagcctgca ggaccctacg gagctcgccc 181 cccagcaggt gccaggaata aagttcaaca tcaggccaag gcagccccac cacgacctcc 241 caccaggcgg ctcccaggat ggtgacttga aggaacccac agagagggtc actcgggact 301 tatccagtgg ggccccgagg ggccgcaacc tgccagcgcc tgaccagcct caacccccgc 361 tgcagagggg aacccgtctg cggctccgcc agcgccgtcg ccgtctgctc atcaagaaaa 421 tgccagctgc ggcgaccatc ccggccaaca gctcggacgc gcccttcatc cggccgggac 481 ccgggacgct ggatggccgc tgggtcagcc tgcaccggag ccagcaggag cgcaagcggg 541 tgatgcagga ggcctgcgcc aagtaccggg cgagcagcag ccgccgggcc gtcacgcccc 601 gccacgtgtc ccgtatcttc gtggaggacc gccaccgcgt gctctactgc gaggtgccca 661 aggccggctg ctccaattgg aagcgggtgc tcatggtgct ggccggcctg gcctcgtcca 721 ctgccgacat ccagcacaac accgtccact atggcagcgc tctcaagcgc ctggacacct 781 tcgaccgcca gggtatcttg caccgtctca gcacctacac caagatgctc tttgtccgcg 841 agcccttcga gaggctggtg tccgccttcc gcgacaagtt tgagcacccc aacagctact 901 atcacccggt cttcggcaag gccatcctgg cccggtaccg cgccaatgcc tctcgggagg 961 cccTGCGGAC CGGCTCTGGG GTGCGTTTTC CCGAGTTCGT CCAGTACCTG CTGGACGTGC 1021 ACCGGCCCGT GGGGATGGAC ATTCACTGGG ACCATGTCAG CCGGCTCTGC AGCCCCTGCC 1081 TCATCGACTA CGATTTCGTA GGCAAGTTCG AGAGCATGGA GGACGATGCC AACTTCTTCC 1141 TGAGCCTCAT CCGCGCGCCG CGGAACCTGA CCTTCCCCCG GTTCAAGGAC CGGCACTCGC 1201 AGGAGGCGCG GACCACAGCG AGGATCGCCC ACCAGTACTT CGCCCAACTC TCGGCCCTGC 1261 AAAGGCAGCG CACCTACGAC TTCTACTACA TGGATTACCT GATGTTCAAC TATTCCAAGC 1321 CCTTTGCAGA TCTGTACTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1381 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1441 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATGTGCCT CATCCAGAAC 1501 CACGTATACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt