Construct: ORF TRCN0000480339
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019013.3_s317c1
- Derived from:
- ccsbBroadEn_14819
- DNA Barcode:
- CTGATGCGCCTTTGCCGGGGGAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTK6 (5753)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480339
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5753 | PTK6 | protein tyrosine kinase 6 | NM_005975.4 | 100% | 100% | |
| 2 | human | 5753 | PTK6 | protein tyrosine kinase 6 | XM_017027982.1 | 60.3% | 49.4% | 668_669insAGACAACCTCCTGCACCAGC;816_817ins517 |
| 3 | human | 5753 | PTK6 | protein tyrosine kinase 6 | NM_001256358.1 | 29.7% | 21.3% | 230_231ins122;402_403ins829 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1422
- ORF length:
- 1353
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtgtcccgg gaccaggctc acctgggccc caagtatgtg ggcctctggg 121 acttcaagtc ccggacggac gaggagctga gcttccgcgc gggggacgtc ttccacgtgg 181 ccaggaagga ggagcagtgg tggtgggcca cgctgctgga cgaggcgggt ggggccgtgg 241 cccagggcta tgtgccccac aactacctgg ccgagaggga gacggtggag tcggaaccgt 301 ggttctttgg ctgcatctcc cgctcggaag ctgtgcgtcg gctgcaggcc gagggcaacg 361 ccacgggcgc cttcctgatc agggtcagcg agaagccgag tgccgactac gtcctgtcgg 421 tgcgggacac gcaggctgtg cggcactaca agatctggcg gcgtgccggg ggccggctgc 481 acctgaacga ggcggtgtcc ttcctcagcc tgcccgagct tgtgaactac cacagggccc 541 agagcctgtc ccacggcctg cggctggccg cgccctgccg gaagcacgag cctgagcccc 601 tgccccattg ggatgactgg gagaggccga gggaggagtt cacgctctgc aggaagctgg 661 ggtccggcta ctttggggag gtcttcgagg ggctctggaa agaccgggtc caggtggcca 721 ttaaggtgat ttctcgagac aacctcctgc accagcagat gctgcagtcg gagatccagg 781 ccatgaagaa gctgcggcac aaacacatcc tggcgctgta cgccgtggtg tccgtggggg 841 accccgtgta catcatcacg gagctcatgg ccaagggcag cctgctggag ctgctccgcg 901 actctgatga gaaagtcctg cccgtttcgg agctgctgga catcgcctgg caggtggctg 961 agggcatgtg ttacctggag tcgcagaatt acatccaccg ggacctggcc gccaggaaca 1021 tcctcgtcgg gGAAAACACC CTCTGCAAAG TTGGGGACTT CGGGTTAGCC AGGCTTATCA 1081 AGGAGGACGT CTACCTCTCC CATGACCACA ATATCCCCTA CAAGTGGACG GCCCCTGAAG 1141 CGCTCTCCCG AGGCCATTAC TCCACCAAAT CCGACGTCTG GTCCTTTGGG ATTCTCCTGC 1201 ATGAGATGTT CAGCAGGGGT CAGGTGCCCT ACCCAGGCAT GTCCAACCAT GAGGCCTTCC 1261 TGAGGGTGGA CGCCGGCTAC CGCATGCCCT GCCCTCTGGA GTGCCCGCCC AGCGTGCACA 1321 AGCTGATGCT GACATGCTGG TGCAGGGACC CCGAGCAGAG ACCCTGCTTC AAGGCCCTGC 1381 GGGAGAGGCT CTCCAGCTTC ACCAGCTACG AGAACCCGAC CCCAACTTTC TTGTACAAAG 1441 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1501 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1561 ACGACTGATG CGCCTTTGCC GGGGGAGGAC GCGTTAAGTC gacaatcaac ctctggatta 1621 caaaatttgt gaaagatt