Transcript: Human NM_005975.4

Homo sapiens protein tyrosine kinase 6 (PTK6), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-06-08
Taxon:
Homo sapiens (human)
Gene:
PTK6 (5753)
Length:
2953
CDS:
63..1418

Additional Resources:

NCBI RefSeq record:
NM_005975.4
NBCI Gene record:
PTK6 (5753)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001149364 CGCACCCGACAGGACGTAGT pXPR_003 CGG 340 25% 2 0.6322 PTK6 PTK6 76490
2 BRDN0001162535 GCCGACGCACAGCTTCCGAG pXPR_003 CGG 257 19% 2 0.2955 PTK6 PTK6 76493
3 BRDN0001145574 CTCTCCCAGTCATCCCAATG pXPR_003 GGG 540 40% 4 0.2576 PTK6 PTK6 76492
4 BRDN0001162471 CGTGGAAGACGTCCCCCGCG pXPR_003 CGG 92 7% 1 -0.1402 PTK6 PTK6 76491
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_005975.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000381802 AGGCCATTACTCCACCAAATC pLKO_005 1145 CDS 100% 10.800 15.120 N PTK6 n/a
2 TRCN0000199336 CGGGTCCAGGTGGCCATTAAG pLKO.1 699 CDS 100% 0.000 0.000 N PTK6 n/a
3 TRCN0000379988 GGATTCTCCTGCATGAGATGT pLKO_005 1183 CDS 100% 4.950 3.960 N PTK6 n/a
4 TRCN0000199550 CCCAGGCATGTCCAACCATGA pLKO.1 1226 CDS 100% 1.350 1.080 N PTK6 n/a
5 TRCN0000021551 ACCTCTCCCATGACCACAATA pLKO.1 1087 CDS 100% 13.200 9.240 N PTK6 n/a
6 TRCN0000314824 AGGTGGCCATTAAGGTGATTT pLKO_005 706 CDS 100% 13.200 9.240 N PTK6 n/a
7 TRCN0000194770 CATCCATGGTTAAGTCATAAA pLKO.1 2162 3UTR 100% 13.200 9.240 N PTK6 n/a
8 TRCN0000314773 CCTCTCCCATGACCACAATAT pLKO_005 1088 CDS 100% 13.200 9.240 N PTK6 n/a
9 TRCN0000196912 GTGCAGGAAAGGTTCACAAAT pLKO.1 1528 3UTR 100% 13.200 9.240 N PTK6 n/a
10 TRCN0000314823 GTGCAGGAAAGGTTCACAAAT pLKO_005 1528 3UTR 100% 13.200 9.240 N PTK6 n/a
11 TRCN0000350386 TTACCTGGAGTCGCAGAATTA pLKO_005 965 CDS 100% 13.200 9.240 N PTK6 n/a
12 TRCN0000314825 AGTCGGAACCGTGGTTCTTTG pLKO_005 283 CDS 100% 10.800 7.560 N PTK6 n/a
13 TRCN0000381105 TCCGCGACTCTGATGAGAAAG pLKO_005 889 CDS 100% 10.800 7.560 N PTK6 n/a
14 TRCN0000021549 CCTGTTTGTCTTGTGTCTTGA pLKO.1 1888 3UTR 100% 4.950 3.465 N PTK6 n/a
15 TRCN0000197094 GCTTATCAAGGAGGACGTCTA pLKO.1 1067 CDS 100% 4.050 2.835 N PTK6 n/a
16 TRCN0000021550 GCCATTAAGGTGATTTCTCGA pLKO.1 711 CDS 100% 2.640 1.848 N PTK6 n/a
17 TRCN0000021553 AGTCGCAGAATTACATCCACC pLKO.1 973 CDS 100% 2.160 1.512 N PTK6 n/a
18 TRCN0000199853 GCTCCGCGACTCTGATGAGAA pLKO.1 887 CDS 100% 1.650 1.155 N PTK6 n/a
19 TRCN0000199057 CCGTGGTTCTTTGGCTGCATC pLKO.1 291 CDS 100% 1.350 0.945 N PTK6 n/a
20 TRCN0000199034 CCCTCACTCCTGCGCTGACAA pLKO.1 2060 3UTR 100% 0.000 0.000 N PTK6 n/a
21 TRCN0000021552 TACCTCTCCCATGACCACAAT pLKO.1 1086 CDS 100% 0.000 0.000 N PTK6 n/a
22 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 2262 3UTR 100% 13.200 6.600 Y LIAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_005975.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14819 pDONR223 0% 100% 100% None n/a
2 ccsbBroad304_14819 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000480339 CTGATGCGCCTTTGCCGGGGGAGG pLX_317 28.9% 100% 100% V5 n/a
4 TRCN0000488198 CCAACTGCGATGAGGTACCACCGT pLX_317 19.2% 100% 100% V5 (not translated due to prior stop codon) n/a
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