Construct: ORF TRCN0000480340
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018284.1_s317c1
- Derived from:
- ccsbBroadEn_03140
- DNA Barcode:
- TAGCGTTCTGGATAAGCGCTCGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WRAP73 (49856)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480340
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 49856 | WRAP73 | WD repeat containing, antis... | NM_017818.4 | 100% | 100% | |
| 2 | human | 49856 | WRAP73 | WD repeat containing, antis... | XM_017001387.2 | 98.4% | 98.2% | 922_923ins21 |
| 3 | human | 49856 | WRAP73 | WD repeat containing, antis... | XM_005244754.2 | 90.2% | 90.2% | 601_602ins135 |
| 4 | human | 49856 | WRAP73 | WD repeat containing, antis... | XR_946661.3 | 78% | (many diffs) | |
| 5 | mouse | 59002 | Wrap73 | WD repeat containing, antis... | NM_021499.2 | 83.5% | 87.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1449
- ORF length:
- 1380
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaacttctcc gaggtattca agctctccag cttactctgc aagttctccc 121 cggacggcaa gtacctggct tcctgtgtcc agtaccggtt agtggtccgg gatgtgaaca 181 cccttcagat ccttcagctg tacacgtgcc tagaccagat ccagcacatc gagtggtcgg 241 cagactcgct cttcatcctg tgcgccatgt acaagcgagg gctggtgcag gtctggtctt 301 tagagcagcc cgaatggcac tgcaaaatag acgagggctc agccgggctg gtggcctcgt 361 gctggagccc ggacgggcgc cacattctca acaccacgga attccatctg cggataaccg 421 tctggtcctt gtgcacaaaa tccgtgtctt acatcaaata cccgaaagct tgtctgcagg 481 gaatcacctt caccagggac ggccgctaca tggcgctggc agaacggcgc gactgcaaag 541 attacgtgag catcttcgtc tgcagtgatt ggcagctcct gcggcatttt gatacggaca 601 cccaggatct cacagggatt gagtgggccc caaacggctg tgtgctggca gtgtgggaca 661 cctgcttgga gtacaagatt ctgctgtact cattggatgg ccggttgttg tccacgtaca 721 gcgcttacga gtggtccctg ggcatcaagt ctgtggcctg gagccccagc agtcagttcc 781 tggcagttgg gagctatgat ggaaaggtgc gcatccttaa tcacgtgact tggaaaatga 841 tcacggagtt tgggcatcct gcagccatta atgatcccaa gatagtggtg tataaggagg 901 ccgagaagag cccacagctg ggactgggct gcctctcctt cccgccgccc cgggccgggg 961 ccggccctct cccgagctca gagagtaaat atgagatcgc ctctgtccca gtctccTTAC 1021 AGACACTGAA ACCTGTTACC GACAGAGCAA ACCCGAAAAT CGGCATAGGA ATGCTGGCAT 1081 TTAGTCCTGA CAGCTACTTC CTGGCGACAA GGAACGACAA CATTCCCAAT GCCGTCTGGG 1141 TCTGGGACAT TCAGAAGCTG AGGCTGTTCG CGGTGCTCGA GCAGCTGTCC CCAGTGCGCG 1201 CATTTCAGTG GGACCCGCAG CAGCCGCGGC TGGCCATCTG CACGGGAGGC AGCAGGCTCT 1261 ACCTGTGGTC CCCAGCGGGC TGCATGTCGG TGCAGGTGCC TGGGGAAGGC GACTTTGCAG 1321 TGCTCTCTCT GTGCTGGCAT TTAAGCGGAG ACTCGATGGC CCTCCTCAGC AAGGATCACT 1381 TCTGCCTCTG CTTCCTGGAG ACAGAGGCAG TGGTCGGCAC AGCCTGCAGA CAGCTGGGCG 1441 GCCACACGTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1501 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1561 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATAGCGT TCTGGATAAG CGCTCGACAC 1621 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt