Construct: ORF TRCN0000480364
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000368.1_s317c1
- Derived from:
- ccsbBroadEn_00056
- DNA Barcode:
- GTTAATATGCACTGCGTAAGTTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALOX5 (240)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480364
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 240 | ALOX5 | arachidonate 5-lipoxygenase | NM_000698.5 | 100% | 100% | |
| 2 | human | 240 | ALOX5 | arachidonate 5-lipoxygenase | NM_001320861.2 | 95.6% | 95.6% | 1270_1271ins87 |
| 3 | human | 240 | ALOX5 | arachidonate 5-lipoxygenase | NM_001256153.3 | 95.2% | 95.2% | 1268_1269ins96 |
| 4 | human | 240 | ALOX5 | arachidonate 5-lipoxygenase | NM_001256154.2 | 91.5% | 91.5% | 1672_1673ins171 |
| 5 | human | 240 | ALOX5 | arachidonate 5-lipoxygenase | NM_001320862.2 | 78.4% | 78.4% | 0_1ins435 |
| 6 | human | 240 | ALOX5 | arachidonate 5-lipoxygenase | XM_017016012.1 | 78.4% | 78.4% | 0_1ins435 |
| 7 | human | 240 | ALOX5 | arachidonate 5-lipoxygenase | XR_001747075.1 | 52.8% | (many diffs) | |
| 8 | mouse | 11689 | Alox5 | arachidonate 5-lipoxygenase | NM_009662.2 | 88% | 93.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2091
- ORF length:
- 2022
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccctcctac acggtcaccg tggccactgg cagccagtgg ttcgccggca 121 ctgacgacta catctacctc agcctcgtgg gctcggcggg ctgcagcgag aagcacctgc 181 tggacaagcc cttctacaac gacttcgagc gtggcgcggt ggattcatac gacgtgactg 241 tggacgagga actgggcgag atccagctgg tcagaatcga gaagcgcaag tactggctga 301 atgacgactg gtacctgaag tacatcacgc tgaagacgcc ccacggggac tacatcgagt 361 tcccctgcta ccgctggatc accggcgatg tcgaggttgt cctgagggat ggacgcgcaa 421 agttggcccg agatgaccaa attcacattc tcaagcaaca ccgacgtaaa gaactggaaa 481 cacggcaaaa acaatatcga tggatggagt ggaaccctgg cttccccttg agcatcgatg 541 ccaaatgcca caaggattta ccccgtgata tccagtttga tagtgaaaaa ggagtggact 601 ttgttctgaa ttactccaaa gcgatggaga acctgttcat caaccgcttc atgcacatgt 661 tccagtcttc ttggaatgac ttcgccgact ttgagaaaat ctttgtcaag atcagcaaca 721 ctatttctga gcgggtcatg aatcactggc aggaagacct gatgtttggc taccagttcc 781 tgaatggctg caaccctgtg ttgatccggc gctgcacaga gctgcccgag aagctcccgg 841 tgaccacgga gatggtagag tgcagcctgg agcggcagct cagcttggag caggaggtcc 901 agcaagggaa cattttcatc gtggactttg agctgctgga tggcatcgat gccaacaaaa 961 cagacccctg cacactccag ttcctggccg ctcccatctg cttgctgtat aagaacctgg 1021 ccaacaagat tgtccccatt gccatccagc tcaaccaaat cccgggagat gagaacccta 1081 ttttcctccc ttcggatgca aaatacgact ggcttttggc caaaatctgg gtgcgttcca 1141 gtgacttcca cgtccaccag accatcaccc accttctgcg aacacatctg gtgtctgagg 1201 tttttggcat tgcaatgtac cgccagctgc ctgctgtgca ccccattttc aagctgctgg 1261 tggcacacgt gagattcacc attgcaatca acaccaaggc ccgtgagcag ctcatctgcg 1321 agtgtggcct ctttgacaag gccaacgcca cagggggcgg tgggcacgtg cagatggtgc 1381 agagggccat gaaggacctg acctatgcct ccctgtgctt tcccgaggcc atcaaggccc 1441 ggggcatgga gagcaaagaa gacatcccct actacttcta ccgggacgac gggctcctgg 1501 tgtgggaagc catcaggacg ttcacggccg aggtggtaga catctactac gagggcgacc 1561 aggtggtgga ggaggacccg gagctgcagg acttcgtgaa cgatgtctac gtgtacggca 1621 tgcggggccg caagtcctca ggcttcccca agtcggtcaa gagccgggag cagctgtcgg 1681 agtacctgac cgtggtgatc ttcaccgccT CCGCCCAGCA CGCCGCGGTC AACTTCGGCC 1741 AGTACGACTG GTGCTCCTGG ATCCCCAATG CGCCCCCAAC CATGCGAGCC CCGCCACCGA 1801 CTGCCAAGGG CGTGGTGACC ATTGAGCAGA TCGTGGACAC GCTGCCCGAC CGCGGCCGCT 1861 CCTGCTGGCA TCTGGGTGCA GTGTGGGCGC TGAGCCAGTT CCAGGAAAAC GAGCTGTTCC 1921 TGGGCATGTA CCCAGAAGAG CATTTTATCG AGAAGCCTGT GAAGGAAGCC ATGGCCCGAT 1981 TCCGCAAGAA CCTCGAGGCC ATTGTCAGCG TGATTGCTGA GCGCAACAAG AAGAAGCAGC 2041 TGCCATATTA CTACTTGTCC CCAGACCGGA TTCCGAACAG TGTGGCCATC TTGCCAACTT 2101 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2161 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2221 CTTGTGGAAA GGACGAGTTA ATATGCACTG CGTAAGTTCG ACGCGTTAAG TCgacaatca 2281 acctctggat tacaaaattt gtgaaagatt