Construct: ORF TRCN0000480375

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010365.1_s317c1
Derived from:
ccsbBroadEn_03391
DNA Barcode:
CCTACCATGGGAACCTAGAGTCGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRKAG3 (53632)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480375
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 53632 PRKAG3 protein kinase AMP-activate... NM_017431.3 100% 100%
2 human 53632 PRKAG3 protein kinase AMP-activate... XM_017004343.2 100% 100%
3 mouse 241113 Prkag3 protein kinase, AMP-activat... NM_153744.3 85.4% 84.6% (many diffs)
4 mouse 241113 Prkag3 protein kinase, AMP-activat... XM_006495988.3 85.4% 84.6% (many diffs)
5 mouse 241113 Prkag3 protein kinase, AMP-activat... XM_006495989.3 85.4% 84.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1536
ORF length:
1467
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggagcccggg ctggagcacg cactgcgcag gaccccttcc tggagcagcc
121ttgggggttc tgagcatcaa gagatgagct tcctagagca agaaaacagc agctcatggc
181catcaccagc tgtgaccagc agctcagaaa gaatccgtgg gaaacggagg gccaaagcct
241tgagatggac aaggcagaag tcggtggagg aaggggagcc accaggtcag ggggaaggtc
301cccggtccag gccagctgct gagtccaccg ggctggaggc cacattcccc aagaccacac
361ccttggctca agctgatcct gccggggtgg gcactccacc aacagggtgg gactgcctcc
421cctctgactg tacagcctca gctgcaggct ccagcacaga tgatgtggag ctggccacgg
481agttcccagc cacagaggcc tgggagtgtg agctagaagg cctgctggaa gagaggcctg
541ccctgtgcct gtccccgcag gccccatttc ccaagctggg ctgggatgac gaactgcgga
601aacccggcgc ccagatctac atgcgcttca tgcaggagca cacctgctac gatgccatgg
661caactagctc caagctagtc atcttcgaca ccatgctgga gatcaagaag gccttctttg
721ctctggtggc caacggtgtg cgggcagccc ctctatggga cagcaagaag cagagctttg
781tggggatgct gaccatcact gacttcatcc tggtgctgca tcgctactac aggtcccccc
841tggtccagat ctatgagatt gaacaacata agattgagac ctggagggag atctacctgc
901aaggctgctt caagcctctg gtctccatct ctcctaatga tagcctgttt gaagctgtct
961acaccctcat caagaaccgg atccatcgcc tgcctgttct tgacccggtg tcaggcaacg
1021tactccacat cctcacacac aaacgcctgc tcaagttcct gcacatcttt ggttccctgc
1081tgccccggcc ctccttcctc taccgcacta tccaagattt gggcatcggc acattccgag
1141acttGGCTGT GGTGCTGGAG ACAGCACCCA TCCTGACTGC ACTGGACATC TTTGTGGACC
1201GGCGTGTGTC TGCACTGCCT GTGGTCAACG AATGTGGTCA GGTCGTGGGC CTCTATTCCC
1261GCTTTGATGT GATTCACCTG GCTGCCCAGC AAACCTACAA CCACCTGGAC ATGAGTGTGG
1321GAGAAGCCCT GAGGCAGAGG ACACTATGTC TGGAGGGAGT CCTTTCCTGC CAGCCCCACG
1381AGAGCTTGGG GGAAGTGATC GACAGGATTG CTCGGGAGCA GGTACACAGG CTGGTGCTAG
1441TGGACGAGAC CCAGCATCTC TTGGGCGTGG TCTCCCTCTC CGACATCCTT CAGGCACTGG
1501TGCTCAGCCC TGCTGGCATC GATGCCCTCG GGGCCTTGCC AACTTTCTTG TACAAAGTGG
1561TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
1621CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
1681ACCTACCATG GGAACCTAGA GTCGCACGCG TTAAGTCgac aatcaacctc tggattacaa
1741aatttgtgaa agatt

Download FASTA (ORF) (Full)