Construct: ORF TRCN0000480378
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008978.1_s317c1
- Derived from:
- ccsbBroadEn_01354
- DNA Barcode:
- CGCTCAATGTCCTCACGCTCAAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PYGB (5834)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480378
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5834 | PYGB | glycogen phosphorylase B | NM_002862.4 | 100% | 100% | |
| 2 | human | 5834 | PYGB | glycogen phosphorylase B | XR_002958496.1 | 58.9% | (many diffs) | |
| 3 | mouse | 110078 | Pygb | brain glycogen phosphorylase | NM_153781.1 | 88.6% | 95.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2595
- ORF length:
- 2529
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gaagccgctg acggacagcg agaagcggaa gcagatcagc gtgcgcggcc 121 tggcggggct aggcgacgtg gccgaggtgc ggaagagctt caaccggcac ttgcacttca 181 cgctggtcaa ggaccgcaat gtggccacgc cccgcgacta cttcttcgcg ctggcgcaca 241 cggtgcgcga ccacctcgtg ggccgctgga tccgcacgca gcagcactac tacgagcgcg 301 accccaagcg catttattat ctttccctgg aattctacat gggtcgcacg ctgcagaaca 361 cgatggtgaa cctgggcctt cagaatgcct gcgatgaagc catctatcag ttggggttag 421 acttggagga actcgaggag atagaagaag atgctggcct tgggaatgga ggcctgggga 481 ggctggcagc gtgtttcctt gactcaatgg ctaccttggg cctggcagca tacggctatg 541 gaatccgcta tgaatttggg atttttaacc agaagattgt caatggctgg caggtagagg 601 aggccgatga ctggctgcgc tacggcaacc cctgggagaa agcgcggcct gagtatatgc 661 ttcccgtgca cttctacgga cgcgtggagc acacccccga cggcgtgaag tggctggaca 721 cacaggtggt gctggccatg ccctacgaca ccccagtgcc cggctacaag aacaacaccg 781 tcaacaccat gcggctgtgg tccgccaagg ctcccaacga cttcaagctg caggacttca 841 acgtgggaga ctacatcgag gcggtcctgg accggaactt ggctgagaac atctccaggg 901 tcctgtatcc aaatgataac ttctttgagg ggaaggagct gcggctgaag caggagtact 961 tcgtggtggc cgccacgctc caggacatca tccgccgctt caagtcgtcc aagttcggct 1021 gccgggaccc tgtgagaacc tgtttcgaga cgttcccaga caaggtggcc atccagctga 1081 acgacaccca ccccgccctc tccatccctg agctcatgcg gatcctggtg gacgtggaga 1141 aggtggactg ggacaaggcc tgggaaatca cgaagaagac ctgtgcatac accaaccaca 1201 ctgtgctgcc tgaggccttg gagcgctggc ccgtgtccat gtttgagaag ctgctgccgc 1261 ggcacctgga gataatctat gccatcaacc agcggcacct ggaccacgtg gccgcgctgt 1321 ttcccggcga tgtggaccgc ctgcgcagga tgtctgtgat cgaggagggg gactgcaagc 1381 ggatcaacat ggcccacctg tgtgtgattg ggtcccatgc tgtcaatggt gtggcgagga 1441 tccactcgga gatcgtgaaa cagtcggtct ttaaggattt ttatgaactg gagccagaga 1501 agttccagaa taagaccaat ggcatcaccc cccgccggtg gctgctgctg tgcaacccgg 1561 ggctggccga taccatcgtg gagaaaattg gggaggagtt cctgactgac ctgagccagc 1621 tgaagaagct gctgccgctg gtcagtgacg aggtgttcat cagggacgtg gccaaggtca 1681 aacaggagaa caagctcaag ttctcggcct tcctggagaa ggagtacaag gtgaagatca 1741 acccctcctc catgttcgat gtgcatgtga agaggatcca cgagtacaag cggcagctgc 1801 tcaactgcct gcacgtcgtc accctgtaca atcgaatcaa gagagacccg gccaaggctt 1861 ttgtgcccag gactgttatg attgggggca aggcagcgcc cggttaccac atggccaagc 1921 tgatcatcaa gttggtcacc tccatcggcg acgtcgtcaa tcatgaccca gttgtgggtg 1981 acaggttgaa agtgatcttc ctggagaact accgtgtgtc cttggctgag aaagtgatcc 2041 cggccgctga tctgtcgcag cagatctcca ctgcaggcac cgaggcctca ggcacaggca 2101 acatgaagtt catgctcaac ggggccctca ccatcggcac catggacggc gccaacgtgg 2161 agatggccga ggaggccggg gccgagaacc tcttcatctt cggccTGCGG GTGGAGGATG 2221 TCGAGGCCTT GGACCGGAAA GGGTACAATG CCAGGGAGTA CTACGACCAC CTGCCCGAGC 2281 TGAAGCAGGC CGTGGACCAG ATCAGCAGTG GCTTTTTTTC TCCCAAGGAG CCAGACTGCT 2341 TCAAGGACAT CGTGAACATG CTGATGCACC ATGACAGGTT CAAGGTGTTT GCAGACTATG 2401 AAGCCTACAT GCAGTGCCAG GCACAGGTGG ACCAGCTGTA CCGGAACCCC AAGGAGTGGA 2461 CCAAGAAGGT CATCAGGAAC ATCGCCTGCT CGGGCAAGTT CTCCAGTGAC CGGACCATCA 2521 CGGAGTATGC ACGGGAGATC TGGGGTGTGG AGCCCTCCGA CCTGCAGATC CCGCCCCCCA 2581 ACATCCCCCG GGACTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2641 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2701 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CGCTCAATGT CCTCACGCTC 2761 AAGTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt