Construct: ORF TRCN0000480386
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001542.1_s317c1
- Derived from:
- ccsbBroadEn_02258
- DNA Barcode:
- GATGCATTTGGTTTGTCTGCCCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZBTB24 (9841)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480386
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9841 | ZBTB24 | zinc finger and BTB domain ... | NM_014797.2 | 100% | 100% | |
| 2 | human | 9841 | ZBTB24 | zinc finger and BTB domain ... | NM_001164313.1 | 46.7% | 45.5% | (many diffs) |
| 3 | mouse | 268294 | Zbtb24 | zinc finger and BTB domain ... | XM_006512748.2 | 57.6% | 55.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2160
- ORF length:
- 2091
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcagaaaca tcgccagagc cttctgggca gcttgttgta cactcagacg 121 ctcacagtga cactgtgctg gccagttttg aggatcagag gaagaaaggc ttcctctgtg 181 acattacttt aatcgtggag aatgtacatt tccgggccca caaagcctta cttgctgcca 241 gtagtgaata cttctcaatg atgtttgcag aagaggggga aatcggccaa tccatttata 301 tgctggaagg catggttgca gacacctttg gtatcctgct ggaatttatc tacacaggtt 361 atctccatgc cagtgagaaa agtacagaac aaatcctggc tactgctcag ttcttaaaag 421 tctatgacct ggtaaaggct tacacagact tccaaaataa tcatagctcc ccaaagccaa 481 caactttgaa cactgctggt gccccagtgg ttgttatctc taataagaaa aacgatcctc 541 caaagcggaa acggggaaga ccaaaaaaag tcaatacatt gcaggaggag aaatcagaac 601 tggctgcaga ggaagaaata cagttaagag tgaacaattc agttcagaat agacaaaact 661 ttgtggttaa aggagacagt ggtgtactga atgagcaaat tgcagcaaaa gaaaaggaag 721 aatcggagcc tacttgtgag ccaagtagag aggaggaaat gccagttgaa aaagatgaga 781 actatgatcc caagaccgag gatggccagg caagccagag tcgatacagc aagcggagga 841 tttggagatc cgtcaaactt aaagattaca aacttgttgg ggatcaagag gaccatggtt 901 cagccaagag gatctgtgga aggagaaagc gccctggagg ccctgaggcc cgctgtaaag 961 actgtggcaa ggtctttaag tacaatcact ttttagcaat ccaccagagg agccacacag 1021 gggagcgacc tttcaaatgt aatgagtgtg gaaaaggctt tgcccagaag cactcgctac 1081 aggtccacac caggatgcac acaggcgagc ggccgtacac ctgcaccgtg tgcagcaagg 1141 ctctgaccac caagcactca ctgctggagc acatgagcct gcactcagga cagaagtctt 1201 ttacctgtga tcaatgcgga aaatatttca gccagaacag acagctaaag agccattacc 1261 gagttcatac aggccactca ttaccggaat gcaaagactg ccatcgcaaa ttcatggatg 1321 tgtctcagct aaagaaacat ctgcgaacac acacaggtga gaagccattt acttgtgaaa 1381 tctgtggcaa atctttcaca gcaaagagtt ctcttcagac ccacatcaga atccatcgag 1441 gagaaaagcc atactcctgt ggcatttgtg gcaaatcctt ctctgactcc agtgccaaaa 1501 ggagacactg cattctacac actggcaaga agcctttctc ctgccctgag tgtaacttac 1561 agtttgctcg cttagacaac ttgaaggctc acttgaaaat tcatagcaag gagaagcatg 1621 cttcagatgc cagcagcatt tctggcagta gtaatacaga agaggtcagg aatattcttc 1681 agctacagcc atatcaactc tctacctcgg gagagcagga aattcagctt ctcgtaaccg 1741 attctgtaca taacatcaat TTCATGCCCG GTCCTAGCCA GGGAATCAGC ATTGTGACTG 1801 CAGAGAGTTC CCAAAACATG ACTGCAGACC AGGCTGCTAA TCTTACCCTG CTCACGCAGC 1861 AGCCAGAGCA ACTGCAGAAT TTAATTCTTT CAGCTCAACA GGAGCAAACA GAACACATTC 1921 AGAGCCTCAA TATGATTGAA AGCCAGATGG GGCCCTCACA AACAGAGCCA GTGCACGTGA 1981 TCACTCTGTC CAAGGAAACA CTGGAACATC TTCATGCCCA TCAAGAGCAA ACAGAGGAGC 2041 TCCATTTAGC TACAAGTACT TCAGATCCAG CTCAGCACCT GCAGCTGACA CAGGAGCCTG 2101 GTCCGCCACC ACCCACTCAC CACGTGCCCC AGCCAACGCC ACTTGGCCAG GAGCAGAGCT 2161 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2221 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2281 TTTATATATC TTGTGGAAAG GACGAGATGC ATTTGGTTTG TCTGCCCTCA CGCGTTAAGT 2341 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt