Construct: ORF TRCN0000480390
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018914.2_s317c1
- Derived from:
- ccsbBroadEn_14863
- DNA Barcode:
- GAGTTTGCGAAGGGGGGGTGCGCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- TGFBR3 (7049)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480390
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7049 | TGFBR3 | transforming growth factor ... | NM_003243.5 | 99.2% | 28% | (many diffs) |
| 2 | human | 7049 | TGFBR3 | transforming growth factor ... | XM_006710867.2 | 99.2% | 28% | (many diffs) |
| 3 | human | 7049 | TGFBR3 | transforming growth factor ... | NM_001195683.2 | 99.1% | 28% | (many diffs) |
| 4 | human | 7049 | TGFBR3 | transforming growth factor ... | NM_001195684.1 | 99.1% | 28% | (many diffs) |
| 5 | human | 7049 | TGFBR3 | transforming growth factor ... | NR_036634.2 | 39.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 828
- ORF length:
- 759
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacttcccat tatgtgattg ccatctttgc cctgatgagc ttctgtttag 121 ccactgcagg tccagagcct ggtgcactgt gtgaactgtc acctgtcagt gcctcccatc 181 ctgtccaggc cttgatggag agcttcactg ttttgtcagg ctgtgccagc agaggcacaa 241 ctgggctgcc acaggaggtg catgtcctga atctccgcac tgcgggccag gggcctggcc 301 agctacagag agaggtcaca cttcacctga atcccatctc ctcagtccac atccaccaca 361 agtctgttgt gttcctgctc aactccccac accccctggt gtggcatctg aagacagaga 421 gacttgccac tggggtctcc agactgtttt tggtgtctga gggttctgtg gtccagtttt 481 catcagcaaa cttctccttg acagcagaaa cagaagaaag gaacttcccc catggaaatg 541 aacatctgtt aaattgggcc cgaaaagagt atggagcagt tacttcattc accgaactca 601 agatagcaag aaacatttat attaaagtgg gggaagatca agtgttccct ccaaagtgca 661 acatagggaa gaattttctc tcactcaatt accttgctga gtaccttcaa cccaaagcag 721 cagaacggtg tgtgatgtcc agccagcccc agaatgagga agtacacatc atcgagctaa 781 tcaccccaac tctaacccct acagtgcttt ccagtggata taacaattga tatagacctt 841 ctcagaggat ccttgaagtg gtcaaaaatc tcatcctgat cttgaagtgc aaaaagtctg 901 tcaactgggt gatcaaatct tttgatgtta agggaagcct gaaaattatt gctcctaaca 961 gtattggctt tggaaaagag agtgaaagat ctatgacaat gaccaaatca ataagagatg 1021 acattccttc aacccaaggg aatctggtga agtgggcttt ggacaatggc tatagtccaa 1081 taacttcata cacaatggct cctgtggcta atagatttca tcttcggctt gaaaataatg 1141 cagaggagat gggagatgag gaagtccaca ctattcctcc tgagctacgg atcctgctgg 1201 accctggtgc cctgcctgcc ctgcagaacc cgcccatccg gggaggggaa ggccaaaatg 1261 gaggccttcc gtttcctttc ccagatattt ccaggagagt ctggaatgaa gagggagaag 1321 atgggctccc tcggccaaag gaccctgtca ttcccagcat acaactgttt cctggtctca 1381 gagagccaga agaggtgcaa gggagcgtgg atattgccct gtctgtcaaa tgtgacaatg 1441 agaagatgat cgtggctgta gaaaaagatt cttttcaggc cagtggctac tcggggatgg 1501 acgtcaccct gttggatcct acctgcaagg ccaagatgaa tggcacacac tttgttttgg 1561 agtctcctct gaatggctgc ggtactcggc cccggtggtc agcccttgat ggtgtggtct 1621 actataactc cattgtgata caggttccag cccttgggga cagtagtggt tggccagatg 1681 gttatgaaga tctggagtca ggtgataatg gatttccggg agatatggat gaaggagatg 1741 cttccctgtt cacccgacct gaaatcgtgg tgtttaattg cagccttcag caggtgagga 1801 accccagcag cttccaggaa cagccccacg ggaacatcac ctttcaacat ggagctatac 1861 accactgacc ctcttttggt gcctccccag gggcgtctct ctgtgccaga gaatggacac 1921 gtttatgttg aggtatctgt tactaaggct gaacaagaac tgggatttgc catccaaaac 1981 gtgctttatc tctccatatt cgaaccctga taggatgtct cattacacca ttattgagaa 2041 tatttgtcct aaagatgaat ctgtgaaatt ctacagtccc aagagagtgc actttcctat 2101 cccgcaagct gacatggata agaagcgatt cagctttgtc ttcaagcctg tcttcaacac 2161 ctcactgctc tttctacagt gtgagctgac gctgtgtacg aagatggaga agcaccccca 2221 gaagttgccT AAGTGTGTGC CTCCTGACGA AGCCTGCACC TCGCTGGACG CCTCGATAAT 2281 CTGGGCCATG ATGCAGAATA AGAAGACGTT CACTAAGCCC CTTGCTGTGA TCCACCATGA 2341 AGCAGAATCT AAAGAAAAAG GTCCAAGCAT GAAGGAACCA AATCCAATTT CTCCACCAAT 2401 TTTCCATGGT CTGGACACCC TAACCGTGAT GGGCATTGCG TTTGCAGCCT TTGTGATCGG 2461 AGCACTCCTG ACGGGGGCCT TGTGGTACAT CTATTCTCAC ACAGGGGAGA CAGCAGGAAG 2521 GCAGCAAGTC CCCACCTCCC CGCCAGCCTC GGAAAACAGC AGTGCTGCCC ACAGCATCGG 2581 CAGCACGCAG AGCACGCCTT GCTCCAGCAG CAGCACGGCC TTGCCAACTT TCTTGTACAA 2641 AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA 2701 ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA 2761 GGACGAGAGT TTGCGAAGGG GGGGTGCGCG ACGCGTTAAG TCgacaatca acctctggat 2821 tacaaaattt gtgaaagatt