Construct: ORF TRCN0000480392
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017417.1_s317c1
- Derived from:
- ccsbBroadEn_05224
- DNA Barcode:
- CACCTCCTCGTGTCCAACACTACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- YPEL4 (219539)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480392
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 219539 | YPEL4 | yippee like 4 | NM_001363487.1 | 100% | 100% | |
| 2 | human | 219539 | YPEL4 | yippee like 4 | NM_145008.3 | 100% | 100% | |
| 3 | mouse | 241525 | Ypel4 | yippee-like 4 (Drosophila) | NM_001005342.2 | 91% | 99.2% | (many diffs) |
| 4 | mouse | 241525 | Ypel4 | yippee-like 4 (Drosophila) | XM_006499466.3 | 91% | 99.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 450
- ORF length:
- 381
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcccagctgt gaccccggTC CGGGCCCTGC CTGCCTCCCC ACCAAGACTT 121 TCCGCAGCTA TCTGCCCCGC TGTCACCGCA CTTACAGCTG TGTCCACTGC CGTGCACACC 181 TGGCCAAACA CGATGAGCTT ATTTCCAAGT CCTTCCAAGG GAGCCATGGC CGAGCCTACC 241 TGTTTAACTC CGTGGTCAAC GTGGGTTGCG GGCCAGCTGA ACAGCGCCTC TTGCTCACGG 301 GGCTCCACTC GGTAGCTGAC ATTTTCTGTG AGAGCTGCAA AACCACACTG GGCTGGAAAT 361 ATGAGCAAGC TTTTGAGACG AGCCAGAAGT ACAAGGAAGG GAAATACATC ATTGAAATGT 421 CACACATGGT GAAGGACAAC GGCTGGGACT TGCCAACTTT CTTGTACAAA GTGGTTGATA 481 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 541 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACACCT 601 CCTCGTGTCC AACACTACTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 661 tgaaagatt