Transcript: Mouse NM_001005342.2

Mus musculus yippee-like 4 (Drosophila) (Ypel4), mRNA.

Source:
NCBI, updated 2017-05-13
Taxon:
Mus musculus (mouse)
Gene:
Ypel4 (241525)
Length:
752
CDS:
277..660

Additional Resources:

NCBI RefSeq record:
NM_001005342.2
NBCI Gene record:
Ypel4 (241525)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001005342.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000138010 GTTTAACTCCGTGGTCAACGT pLKO.1 450 CDS 100% 2.640 3.696 N YPEL4 n/a
2 TRCN0000175551 CATGTTTACCCACCAAGACTT pLKO.1 308 CDS 100% 4.950 3.960 N Ypel4 n/a
3 TRCN0000176147 GAGACCAGCCAGAAATACAAA pLKO.1 583 CDS 100% 5.625 3.938 N Ypel4 n/a
4 TRCN0000174445 CAAAGAAGGGAAGTACATCAT pLKO.1 600 CDS 100% 4.950 3.465 N Ypel4 n/a
5 TRCN0000174612 GAAATACAAAGAAGGGAAGTA pLKO.1 594 CDS 100% 4.950 3.465 N Ypel4 n/a
6 TRCN0000138054 CCTGTTTAACTCCGTGGTCAA pLKO.1 447 CDS 100% 4.050 2.835 N YPEL4 n/a
7 TRCN0000174533 CTTATTTCCAAGTCCTTCCAA pLKO.1 406 CDS 100% 3.000 1.800 N Ypel4 n/a
8 TRCN0000176001 GCCAGAAATACAAAGAAGGGA pLKO.1 590 CDS 100% 0.750 0.450 N Ypel4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001005342.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05224 pDONR223 100% 91% 99.2% None (many diffs) n/a
2 ccsbBroad304_05224 pLX_304 0% 91% 99.2% V5 (many diffs) n/a
3 TRCN0000480392 CACCTCCTCGTGTCCAACACTACT pLX_317 92% 91% 99.2% V5 (many diffs) n/a
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