Construct: ORF TRCN0000480398

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014372.1_s317c1
Derived from:
ccsbBroadEn_10896
DNA Barcode:
CGGGCTGAATCCCCTGACTACATT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ICA1 (3382)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480398
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3382 ICA1 islet cell autoantigen 1 NM_001276478.2 100% 100%
2 human 3382 ICA1 islet cell autoantigen 1 NM_001350821.2 100% 100%
3 human 3382 ICA1 islet cell autoantigen 1 NM_001350823.2 100% 100%
4 human 3382 ICA1 islet cell autoantigen 1 NM_001350824.2 100% 100%
5 human 3382 ICA1 islet cell autoantigen 1 NM_001350825.2 100% 100%
6 human 3382 ICA1 islet cell autoantigen 1 NM_001136020.3 99.7% 99.7% 17_19delGCA
7 human 3382 ICA1 islet cell autoantigen 1 NM_001350819.2 99.7% 99.7% 17_19delGCA
8 human 3382 ICA1 islet cell autoantigen 1 NM_001350820.2 99.7% 99.7% 17_19delGCA
9 human 3382 ICA1 islet cell autoantigen 1 NM_004968.3 99.7% 99.7% 17_19delGCA
10 human 3382 ICA1 islet cell autoantigen 1 NM_022307.3 99.7% 99.7% 17_19delGCA
11 human 3382 ICA1 islet cell autoantigen 1 NM_001350834.2 95.6% 95.4% 952_953ins63
12 human 3382 ICA1 islet cell autoantigen 1 NM_001350831.2 95.4% 95.2% 17_19delGCA;955_956ins63
13 human 3382 ICA1 islet cell autoantigen 1 NM_001350832.2 95.4% 95.2% 17_19delGCA;955_956ins63
14 human 3382 ICA1 islet cell autoantigen 1 NM_001350833.2 95.4% 95.2% 17_19delGCA;955_956ins63
15 human 3382 ICA1 islet cell autoantigen 1 NM_001350828.2 94.3% 94.3% 900_986del
16 human 3382 ICA1 islet cell autoantigen 1 NM_001350829.2 94.3% 94.3% 900_986del
17 human 3382 ICA1 islet cell autoantigen 1 XM_011515351.1 94.3% 94.3% 900_986del
18 human 3382 ICA1 islet cell autoantigen 1 XM_011515353.2 94.3% 94.3% 900_986del
19 human 3382 ICA1 islet cell autoantigen 1 XM_017012116.1 94.3% 94.3% 900_986del
20 human 3382 ICA1 islet cell autoantigen 1 XM_024446741.1 94.3% 94.3% 900_986del
21 human 3382 ICA1 islet cell autoantigen 1 NM_001350826.2 94.1% 94.1% 17_19delGCA;903_989del
22 human 3382 ICA1 islet cell autoantigen 1 NM_001350827.2 94.1% 94.1% 17_19delGCA;903_989del
23 human 3382 ICA1 islet cell autoantigen 1 XM_011515347.3 94.1% 94.1% 17_19delGCA;903_989del
24 human 3382 ICA1 islet cell autoantigen 1 XM_011515348.1 94.1% 94.1% 17_19delGCA;903_989del
25 human 3382 ICA1 islet cell autoantigen 1 XM_011515349.1 94.1% 94.1% 17_19delGCA;903_989del
26 human 3382 ICA1 islet cell autoantigen 1 NM_001350830.2 90.2% 90% 900_986del;1039_1040ins63
27 human 3382 ICA1 islet cell autoantigen 1 XM_024446740.1 84.7% 84.7% 1_171del;188_190delGCA;1074_1160del
28 human 3382 ICA1 islet cell autoantigen 1 NM_001350838.2 84.2% 84.2% 17_19delGCA;576_577ins225
29 human 3382 ICA1 islet cell autoantigen 1 NM_001350835.2 84.2% 82.5% (many diffs)
30 human 3382 ICA1 islet cell autoantigen 1 NM_001350836.2 84.2% 82.5% (many diffs)
31 human 3382 ICA1 islet cell autoantigen 1 NM_001350837.2 84.2% 82.5% (many diffs)
32 human 3382 ICA1 islet cell autoantigen 1 XM_017012125.1 84.2% 82.5% (many diffs)
33 human 3382 ICA1 islet cell autoantigen 1 XM_011515354.1 79.4% 77.8% (many diffs)
34 human 3382 ICA1 islet cell autoantigen 1 XR_002956426.1 76.2% (many diffs)
35 human 3382 ICA1 islet cell autoantigen 1 NR_146926.2 60.6% 1_153del;1053_1094del;1642_2383del
36 human 3382 ICA1 islet cell autoantigen 1 NR_146929.2 60.6% (many diffs)
37 human 3382 ICA1 islet cell autoantigen 1 NR_146928.2 59.1% 1_153del;1479_1580del;1702_2443del
38 human 3382 ICA1 islet cell autoantigen 1 NR_146927.2 58.9% (many diffs)
39 human 3382 ICA1 islet cell autoantigen 1 XM_011515355.3 53.6% 53.6% 0_1ins624;276_362del
40 human 3382 ICA1 islet cell autoantigen 1 XM_011515356.3 53.6% 53.6% 0_1ins624;276_362del
41 human 3382 ICA1 islet cell autoantigen 1 XM_011515357.2 49.9% 48.5% (many diffs)
42 human 3382 ICA1 islet cell autoantigen 1 XR_002956427.1 46% (many diffs)
43 mouse 15893 Ica1 islet cell autoantigen 1 NM_010492.3 86.5% 89.2% (many diffs)
44 mouse 15893 Ica1 islet cell autoantigen 1 XM_006504993.3 86.5% 89.2% (many diffs)
45 mouse 15893 Ica1 islet cell autoantigen 1 XM_006504994.3 86.5% 89.2% (many diffs)
46 mouse 15893 Ica1 islet cell autoantigen 1 NM_001252266.1 83% 86.3% (many diffs)
47 mouse 15893 Ica1 islet cell autoantigen 1 XM_006504995.3 83% 86.3% (many diffs)
48 mouse 15893 Ica1 islet cell autoantigen 1 XR_001785099.1 44.2% (many diffs)
49 mouse 15893 Ica1 islet cell autoantigen 1 XR_001785098.1 43% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1512
ORF length:
1446
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ctggcatgtc aggacacaaa tgttatccct gggacttaca ggatcgatat gctcaagata
121agtcagttgt aaataagatg caacagaaat attgggagac gaagcaggcc tttattaaag
181ccacagggaa gaaggaagat gaacatgttg ttgcctctga cgcggacctg gatgccaagc
241tagagctgtt tcattcaatt cagagaacct gtctggactt atcgaaagca attgtactct
301atcaaaagag gatatgtttc ttgtctcaag aagaaaacga actgggaaaa tttcttcgat
361cccaaggttt ccaagataaa accagagcag gaaagatgat gcaagcgaca ggaaaggccc
421tctgcttttc ttcccagcaa aggttggcct tacgaaatcc tttgtgtcga tttcaccaag
481aagtggagac ttttcggcat cgggccatct cagatacttg gctgacggtg aaccgcatgg
541aacagtgcag gacggaatat agaggagcac tattatggat gaaggacgtg tctcaggagc
601ttgatccaga cctctacaag caaatggaga agttcaggaa ggtacaaaca caagtgcgcc
661ttgcaaaaaa aaactttgac aaattgaaga tggatgtttg tcaaaaagtg gatcttcttg
721gagcgagcag atgcaatctc ttgtctcaca tgctagcaac ataccagacc actctgcttc
781atttttggga gaaaacttct cacactatgg cagccatcca tgagagtttc aaaggttatc
841aaccatatga atttactact ttaaagagct tacaagaccc tatgaaaaaa ttagttgaga
901aagaagagaa gaagaaaatc aaccagcagg aaagtacaga tgcagccgtg caggagccga
961gccaattaat ttcattagag gaagaaaacc agcgcaagga atcctctagt tttaagactg
1021aagatggaaa aagtatttta tctgccttag acaaaggctc tacacatact gcatgctcag
1081gacccataga tgaactatta gacatgaaaT CTGAGGAAGG TGCTTGCCTG GGACCAGTGG
1141CAGGGACCCC GGAACCTGAA GGTGCTGACA AAGATGACCT GCTGCTGTTG AGTGAGATCT
1201TCAATGCTTC CTCCTTGGAA GAGGGCGAGT TCAGCAAAGA GTGGGCCGCT GTGTTTGGAG
1261ACGGCCAAGT GAAGGAGCCA GTGCCCACTA TGGCCCTGGG AGAGCCAGAC CCCAAGGCCC
1321AGACAGGCTC AGGTTTCCTT CCTTCGCAGC TTTTAGACCA AAATATGAAA GACTTACAGG
1381CCTCGCTACA AGAACCTGCT AAGGCTGCCT CAGACCTGAC TGCCTGGTTC AGCCTCTTCG
1441CTGACCTCGA CCCACTCTCA AATCCTGATG CTGTTGGGAA AACCGATAAA GAACACGAAT
1501TGCTCAATGC ATGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
1561ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
1621CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACGG GCTGAATCCC CTGACTACAT
1681TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)