Construct: ORF TRCN0000480399
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014252.4_s317c1
- Derived from:
- ccsbBroadEn_14887
- DNA Barcode:
- GCCCCCGCTTATCCTTTAGTCGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAPKAPK3 (7867)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480399
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7867 | MAPKAPK3 | MAPK activated protein kina... | NM_001243925.2 | 100% | 100% | |
| 2 | human | 7867 | MAPKAPK3 | MAPK activated protein kina... | NM_001243926.1 | 100% | 100% | |
| 3 | human | 7867 | MAPKAPK3 | MAPK activated protein kina... | NM_004635.4 | 100% | 100% | |
| 4 | human | 7867 | MAPKAPK3 | MAPK activated protein kina... | XR_940501.2 | 40.5% | 1_126del;629_630ins124;1149_2396del | |
| 5 | mouse | 102626 | Mapkapk3 | mitogen-activated protein k... | NM_178907.3 | 89.6% | 94.2% | (many diffs) |
| 6 | mouse | 102626 | Mapkapk3 | mitogen-activated protein k... | XM_006511616.3 | 89.6% | 94.2% | (many diffs) |
| 7 | mouse | 102626 | Mapkapk3 | mitogen-activated protein k... | XM_006511617.3 | 76.4% | 78.6% | (many diffs) |
| 8 | mouse | 102626 | Mapkapk3 | mitogen-activated protein k... | XM_006511618.3 | 75% | 79.1% | (many diffs) |
| 9 | mouse | 102626 | Mapkapk3 | mitogen-activated protein k... | XM_006511619.1 | 65.7% | 70.1% | (many diffs) |
| 10 | mouse | 102626 | Mapkapk3 | mitogen-activated protein k... | NM_001316691.1 | 62.1% | 58% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1212
- ORF length:
- 1146
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tggtgaaaca gcagaggagc aggggggccc tgtgcccccg ccagttgcac 121 ccggcggacc cggcttgggc ggtgctccgg gggggcggcg ggagcccaag aagtacgcag 181 tgaccgacga ctaccagttg tccaagcagg tgctgggcct gggtgtgaac ggcaaagtgc 241 tggagtgctt ccatcggcgc actggacaga agtgtgccct gaagctcctg tatgacagcc 301 ccaaggcccg gcaggaggta gaccatcact ggcaggcttc tggcggcccc catattgtct 361 gcatcctgga tgtgtatgag aacatgcacc atggcaagcg ctgtctcctc atcatcatgg 421 aatgcatgga aggtggtgag ttgttcagca ggattcagga gcgtggcgac caggctttca 481 ctgagagaga agctgcagag ataatgcggg atattggcac tgccatccag tttctgcaca 541 gccataacat tgcccaccga gatgtcaagc ctgaaaacct actctacaca tctaaggaga 601 aagacgcagt gcttaagctc accgattttg gctttgctaa ggagaccacc caaaatgccc 661 tgcagacacc ctgctatact ccctattatg tggcccctga ggtcctgggt ccagagaagt 721 atgacaagtc atgtgacatg tggtccctgg gtgtcatcat gtacatcctc ctttgtggct 781 tcccaccctt ctactccaac acgggccagg ccatctcccc ggggatgaag aggaggattc 841 gcctgggcca gtacggcttc cccaatccTG AGTGGTCAGA AGTCTCTGAG GATGCCAAGC 901 AGCTGATCCG CCTCCTGTTG AAGACAGACC CCACAGAGAG GCTGACCATC ACTCAGTTCA 961 TGAACCACCC CTGGATCAAC CAATCGATGG TAGTGCCACA GACCCCACTC CACACGGCCC 1021 GAGTGCTGCA GGAGGACAAA GACCACTGGG ACGAAGTCAA GGAGGAGATG ACCAGTGCCT 1081 TGGCCACTAT GCGGGTAGAC TACGACCAGG TGAAGATCAA GGACCTGAAG ACCTCTAACA 1141 ACCGGCTCCT CAACAAGAGG AGAAAAAAGC AGGCAGGCAG CTCCTCTGCC TCACAGGGCT 1201 GCAACAACCA GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1261 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1321 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGCC CCCGCTTATC CTTTAGTCGC 1381 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t