Construct: ORF TRCN0000480410
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009334.1_s317c1
- Derived from:
- ccsbBroadEn_04656
- DNA Barcode:
- GCAGCTGGGACCTGCCTGCAGTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LACTB (114294)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480410
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 114294 | LACTB | lactamase beta | NM_032857.5 | 100% | 100% | |
| 2 | human | 114294 | LACTB | lactamase beta | XR_429442.2 | 83.2% | 1_66del;1017_1018insGGTA;1704_1963del | |
| 3 | human | 114294 | LACTB | lactamase beta | XR_931745.2 | 80.2% | 1_66del;1182_1260del;1787_2046del | |
| 4 | human | 114294 | LACTB | lactamase beta | NM_171846.4 | 68.1% | 68.1% | 1119_1119delGins523 |
| 5 | human | 114294 | LACTB | lactamase beta | NM_001288585.2 | 60.6% | 59.2% | 951_952insGGTA;996_997ins641 |
| 6 | mouse | 80907 | Lactb | lactamase, beta | NM_030717.1 | 84.1% | 87.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1710
- ORF length:
- 1641
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtaccggctc atgtcagcag tgactgcccg ggctgccgcc cccgggggct 121 tggcctcaag ctgcggacga cgcggggtcc atcagcgcgc cgggctgccg cctctcggcc 181 acggctgggt cgggggcctc gggctggggc tggggctggc gctcggggtg aagctggcag 241 gtgggctgag gggcgcggcc ccggcgcagt cccccgcggc ccccgaccct gaggcgtcgc 301 ctctggccga gccgccacag gagcagtccc tcgccccgtg gtctccgcag accccggcgc 361 cgccctgctc caggtgcttc gccagagcca tcgagagcag ccgcgacctg ctgcacagga 421 tcaaggatga ggtgggcgca ccgggcatag tggttggagt ttctgtagat ggaaaagaag 481 tctggtcaga aggtttaggt tatgctgatg ttgagaaccg tgtaccatgt aaaccagaga 541 cagttatgcg aattgctagc atcagcaaaa gtctcaccat ggttgctctt gccaaattgt 601 gggaagcagg gaaactggat cttgatattc cagtacaaca ttatgttccc gaattcccag 661 aaaaagaata tgaaggtgaa aaggtttctg tcacaacaag attactgatt tcccatttaa 721 gtggaattcg tcattatgaa aaggacataa aaaaggtgaa agaagagaaa gcttataaag 781 ccttgaagat gatgaaagag aatgttgcat ttgagcaaga aaaagaaggc aaaagtaatg 841 aaaagaatga ttttactaaa tttaaaacag agcaggagaa tgaagccaaa tgccggaatt 901 caaaacctgg caagaaaaag aatgattttg aacaaggcga attatatttg agagaaaagt 961 ttgaaaattc aattgaatcc ctaagattat ttaaaaatga tcctttgttc ttcaaacctg 1021 gtagtcagtt tttgtattca acttttggct ataccctact ggcagccata gtagagagag 1081 cttcaggatg taaatatttg gactatatgc agaaaatatt ccatgacttg gatatgctga 1141 cgactgtgca ggaagaaaac gagccagtga tttacaatag agcaagattt tatgtttaca 1201 ataaaaagaa acgtcttgtc aacacacctt acgtggataa ctcctataaa tgggctggtg 1261 gtggatttct gtctacagtg ggtgaccttc tgaaatttGG GAATGCAATG CTTTATGGTT 1321 ACCAAGTTGG GCTGTTTAAG AACTCAAATG AAAATCTTTT ACCTGGATAC CTCAAACCAG 1381 AAACAATGGT TATGATGTGG ACCCCAGTCC CTAACACAGA GATGTCTTGG GATAAAGAGG 1441 GTAAATATGC AATGGCGTGG GGTGTTGTGG AAAGGAAACA AACGTATGGT TCGTGTAGAA 1501 AGCAACGGCA TTATGCTTCA CATACTGGAG GGGCAGTGGG TGCCAGTAGT GTCCTGCTGG 1561 TCCTTCCTGA AGAACTGGAT ACAGAGACTA TAAATAACAA GGTTCCCCCA AGAGGAATCA 1621 TTGTTTCTAT CATATGTAAC ATGCAATCTG TTGGCCTCAA TAGCACCGCT TTGAAGATTG 1681 CCCTTGAATT TGATAAAGAC AGATCAGACT TGCCAACTTT CTTGTACAAA GTGGTTGATA 1741 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1801 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGCAGC 1861 TGGGACCTGC CTGCAGTCGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1921 tgaaagatt