Construct: ORF TRCN0000480410

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009334.1_s317c1
Derived from:
ccsbBroadEn_04656
DNA Barcode:
GCAGCTGGGACCTGCCTGCAGTCG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LACTB (114294)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480410
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 114294 LACTB lactamase beta NM_032857.5 100% 100%
2 human 114294 LACTB lactamase beta XR_429442.2 83.2% 1_66del;1017_1018insGGTA;1704_1963del
3 human 114294 LACTB lactamase beta XR_931745.2 80.2% 1_66del;1182_1260del;1787_2046del
4 human 114294 LACTB lactamase beta NM_171846.4 68.1% 68.1% 1119_1119delGins523
5 human 114294 LACTB lactamase beta NM_001288585.2 60.6% 59.2% 951_952insGGTA;996_997ins641
6 mouse 80907 Lactb lactamase, beta NM_030717.1 84.1% 87.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1710
ORF length:
1641
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gtaccggctc atgtcagcag tgactgcccg ggctgccgcc cccgggggct
121tggcctcaag ctgcggacga cgcggggtcc atcagcgcgc cgggctgccg cctctcggcc
181acggctgggt cgggggcctc gggctggggc tggggctggc gctcggggtg aagctggcag
241gtgggctgag gggcgcggcc ccggcgcagt cccccgcggc ccccgaccct gaggcgtcgc
301ctctggccga gccgccacag gagcagtccc tcgccccgtg gtctccgcag accccggcgc
361cgccctgctc caggtgcttc gccagagcca tcgagagcag ccgcgacctg ctgcacagga
421tcaaggatga ggtgggcgca ccgggcatag tggttggagt ttctgtagat ggaaaagaag
481tctggtcaga aggtttaggt tatgctgatg ttgagaaccg tgtaccatgt aaaccagaga
541cagttatgcg aattgctagc atcagcaaaa gtctcaccat ggttgctctt gccaaattgt
601gggaagcagg gaaactggat cttgatattc cagtacaaca ttatgttccc gaattcccag
661aaaaagaata tgaaggtgaa aaggtttctg tcacaacaag attactgatt tcccatttaa
721gtggaattcg tcattatgaa aaggacataa aaaaggtgaa agaagagaaa gcttataaag
781ccttgaagat gatgaaagag aatgttgcat ttgagcaaga aaaagaaggc aaaagtaatg
841aaaagaatga ttttactaaa tttaaaacag agcaggagaa tgaagccaaa tgccggaatt
901caaaacctgg caagaaaaag aatgattttg aacaaggcga attatatttg agagaaaagt
961ttgaaaattc aattgaatcc ctaagattat ttaaaaatga tcctttgttc ttcaaacctg
1021gtagtcagtt tttgtattca acttttggct ataccctact ggcagccata gtagagagag
1081cttcaggatg taaatatttg gactatatgc agaaaatatt ccatgacttg gatatgctga
1141cgactgtgca ggaagaaaac gagccagtga tttacaatag agcaagattt tatgtttaca
1201ataaaaagaa acgtcttgtc aacacacctt acgtggataa ctcctataaa tgggctggtg
1261gtggatttct gtctacagtg ggtgaccttc tgaaatttGG GAATGCAATG CTTTATGGTT
1321ACCAAGTTGG GCTGTTTAAG AACTCAAATG AAAATCTTTT ACCTGGATAC CTCAAACCAG
1381AAACAATGGT TATGATGTGG ACCCCAGTCC CTAACACAGA GATGTCTTGG GATAAAGAGG
1441GTAAATATGC AATGGCGTGG GGTGTTGTGG AAAGGAAACA AACGTATGGT TCGTGTAGAA
1501AGCAACGGCA TTATGCTTCA CATACTGGAG GGGCAGTGGG TGCCAGTAGT GTCCTGCTGG
1561TCCTTCCTGA AGAACTGGAT ACAGAGACTA TAAATAACAA GGTTCCCCCA AGAGGAATCA
1621TTGTTTCTAT CATATGTAAC ATGCAATCTG TTGGCCTCAA TAGCACCGCT TTGAAGATTG
1681CCCTTGAATT TGATAAAGAC AGATCAGACT TGCCAACTTT CTTGTACAAA GTGGTTGATA
1741TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
1801CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGCAGC
1861TGGGACCTGC CTGCAGTCGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
1921tgaaagatt

Download FASTA (ORF) (Full)

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