Construct: ORF TRCN0000480417
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005670.1_s317c1
- Derived from:
- ccsbBroadEn_06240
- DNA Barcode:
- TAAGAATAGTCATTTCTTGTCCAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- GABRA4 (2557)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480417
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2557 | GABRA4 | gamma-aminobutyric acid typ... | NM_000809.4 | 99.9% | 99% | 1648delT |
| 2 | human | 2557 | GABRA4 | gamma-aminobutyric acid typ... | NM_001204266.2 | 95.2% | 94.2% | 1_11del;28_29ins68;1591delT |
| 3 | human | 2557 | GABRA4 | gamma-aminobutyric acid typ... | NM_001204267.2 | 86% | 85% | (many diffs) |
| 4 | mouse | 14397 | Gabra4 | gamma-aminobutyric acid (GA... | NM_010251.2 | 87% | 89.5% | (many diffs) |
| 5 | mouse | 14397 | Gabra4 | gamma-aminobutyric acid (GA... | NM_001359041.1 | 83.1% | 85.5% | (many diffs) |
| 6 | mouse | 14397 | Gabra4 | gamma-aminobutyric acid (GA... | NM_001359043.1 | 74.1% | 75.7% | (many diffs) |
| 7 | mouse | 14397 | Gabra4 | gamma-aminobutyric acid (GA... | NM_001359044.1 | 66% | 67% | (many diffs) |
| 8 | mouse | 14397 | Gabra4 | gamma-aminobutyric acid (GA... | NM_001359046.1 | 58.1% | 52% | (many diffs) |
| 9 | mouse | 14397 | Gabra4 | gamma-aminobutyric acid (GA... | NM_001359047.1 | 56% | 51.1% | (many diffs) |
| 10 | mouse | 14397 | Gabra4 | gamma-aminobutyric acid (GA... | NM_001359045.1 | 55.5% | 49.7% | (many diffs) |
| 11 | mouse | 14397 | Gabra4 | gamma-aminobutyric acid (GA... | NM_001359048.1 | 51.9% | 50.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1722
- ORF length:
- 1656
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ttctgccaag aaggtacccg cgatcgctct gtccgccggg gtcagtttcg 121 ccctcctgcg cttcctgtgc ctggcggttt gtttaaacga atccccagga cagaaccaaa 181 aggaggagaa attgtgcaca gaaaatttca cccgcatcct ggacagtttg ctcgatggtt 241 atgacaacag gctgcgtcct ggatttgggg gtcctgttac agaagtgaaa actgacatat 301 atgtcaccag ctttggacct gtttctgatg ttgaaatgga atacacaatg gatgtgttct 361 tcaggcagac atggattgac aaaagattaa aatatgacgg ccccattgaa attttgagat 421 tgaacaatat gatggtaacg aaagtgtgga cccctgatac tttcttcagg aatggaaaga 481 aatctgtctc acataatatg acagctccaa ataagctttt tagaattatg agaaatggta 541 ctattttata cacaatgaga ctcaccataa gtgcggagtg tcccatgaga ttggtggatt 601 ttcccatgga tggtcatgca tgccctttga aattcgggag ttatgcctat ccaaagagtg 661 agatgatcta tacctggaca aaaggtcctg agaaatcagt tgaagttccg aaggagtctt 721 ccagcttagt tcaatatgat ttgattgggc aaaccgtatc aagtgaaacc atcaaatcaa 781 ttacgggtga atatattgtt atgacggttt acttccacct cagacggaag atgggttatt 841 ttatgattca gacctatatt ccgtgcatta tgacagtgat tctttctcaa gtttcatttt 901 ggataaataa agaatcagtt cccgctagga ctgtatttgg aataacaact gtcctcacca 961 tgaccacact aagcatcagt gcacgacatt ctttgcccaa agtgtcctat gctaccgcca 1021 tggactggtt catagctgtc tgctttgctt ttgtattttc ggcccttatc gagtttgctg 1081 ctgtcaacta tttcaccaat attcaaatgg aaaaagccaa aaggaagaca tcaaagcccc 1141 ctcaggaagt tcccgctgct ccagtgcaga gagagaagca tcctgaagcc cctctgcaga 1201 atacaaatgc caatttgaac atgagaaaaa gaacaaatgc tttggttcac tctgaatctg 1261 atgttggcaa cagaactgag gtgggaaacc attcaagcaa atcttccaca gttgttcaag 1321 aatcttctaa aggcacaccT CGGTCTTACT TAGCTTCCAG TCCAAACCCA TTCAGCCGTG 1381 CAAATGCAGC TGAAACCATA TCTGCAGCAA GAGCACTTCC ATCTGCTTCT CCTACTTCTA 1441 TCCGAACTGG ATATATGCCT CGAAAGGCTT CAGTTGGATC TGCTTCTACT CGTCACGTGT 1501 TTGGATCAAG ACTGCAGAGG ATAAAGACCA CAGTTAATAC CATAGGGGCT ACTGGGAAGT 1561 TGTCAGCTAC TCCTCCTCCA TCGGCTCCAC CACCTTCTGG ATCTGGCACA AGTAAAATAG 1621 ACAAATATGC CCGTATTCTC TTTCCAGTCA CATTTGGGGC ATTTAACATG GTTTATTGGG 1681 TTGTTTATTT ATCTAAGGAC ACTATGGAGA AACAGAAAGT CTAATGTACC CAACTTTCTT 1741 GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC 1801 GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG 1861 TGGAAAGGAC GATAAGAATA GTCATTTCTT GTCCACACGC GTTAAGTCga caatcaacct 1921 ctggattaca aaatttgtga aagatt